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Binding of Xenopus transcription factor A to 5S RNA and to single stranded DNA.
Hanas JS
,
Bogenhagen DF
,
Wu CW
.
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Footprint competition assays are utilized to study the binding of Xenopus transcription factor A to a variety of single-stranded nucleic acids. The addition of Xenopus oocyte, yeast, or wheat germ 5S RNA as footprint competitors reveals that factor A binds these 5S RNAs with similar affinity. In contrast, factor A does not bind to E.coli 5S RNA or wheat germ tRNA in this assay. Factor A binding to single stranded DNA is also examined using footprint competition. Factor A binds preferentially to non-specific single stranded (M13) DNA versus double stranded (pBR322) DNA. Factor A binds equally well to single stranded DNA fragments containing either the coding or non-coding strands of the 5S RNA gene. Using single stranded M13 DNA as a competitor, the factor A-5S RNA gene complex is found to dissociate with a half-life of 5-6 min.
Birkenmeier,
A nuclear extract of Xenopus laevis oocytes that accurately transcribes 5S RNA genes.
1978, Pubmed,
Xenbase
Birkenmeier,
A nuclear extract of Xenopus laevis oocytes that accurately transcribes 5S RNA genes.
1978,
Pubmed
,
Xenbase
Bogenhagen,
Stable transcription complexes of Xenopus 5S RNA genes: a means to maintain the differentiated state.
1982,
Pubmed
,
Xenbase
Bogenhagen,
A control region in the center of the 5S RNA gene directs specific initiation of transcription: II. The 3' border of the region.
1980,
Pubmed
,
Xenbase
Bradford,
A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding.
1976,
Pubmed
Brownlee,
The sequence of 5 s ribosomal ribonucleic acid.
1968,
Pubmed
Engelke,
Specific interaction of a purified transcription factor with an internal control region of 5S RNA genes.
1980,
Pubmed
,
Xenbase
Galas,
DNAse footprinting: a simple method for the detection of protein-DNA binding specificity.
1978,
Pubmed
Hanas,
Cooperative model for the binding of Xenopus transcription factor A to the 5S RNA gene.
1983,
Pubmed
,
Xenbase
Korn,
The reactivation of developmentally inert 5S genes in somatic nuclei injected into Xenopus oocytes.
1981,
Pubmed
,
Xenbase
Mackay,
Nucleotide sequences of wheat-embryo cytosol 5-S and 5.8-S ribosomal ribonucleic acids.
1980,
Pubmed
Patton,
A method for isolation of a large amount of a single-stranded DNA fragment.
1982,
Pubmed
Pelham,
A specific transcription factor that can bind either the 5S RNA gene or 5S RNA.
1980,
Pubmed
,
Xenbase
Reynolds,
5S rRNA gene transcription factor IIIA alters the helical configuration of DNA.
1983,
Pubmed
,
Xenbase
Sakonju,
A control region in the center of the 5S RNA gene directs specific initiation of transcription: I. The 5' border of the region.
1980,
Pubmed
,
Xenbase
Sakonju,
Contact points between a positive transcription factor and the Xenopus 5S RNA gene.
1982,
Pubmed
,
Xenbase
Sanger,
Cloning in single-stranded bacteriophage as an aid to rapid DNA sequencing.
1980,
Pubmed
Scheele,
Role of mammalian RNase inhibitor in cell-free protein synthesis.
1979,
Pubmed
Valenzuela,
Nucleotide sequence of the yeast 5S ribosomal RNA gene and adjacent putative control regions.
1977,
Pubmed
Wegnez,
Sequence heterogeneity of 5 S RNA in Xenopus laevis.
1972,
Pubmed
,
Xenbase
