Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
EMBO J
1988 Dec 20;713:4135-40. doi: 10.1002/j.1460-2075.1988.tb03308.x.
Show Gene links
Show Anatomy links
Structure and functional expression of cloned rat serotonin 5HT-2 receptor.
Pritchett DB
,
Bach AW
,
Wozny M
,
Taleb O
,
Dal Toso R
,
Shih JC
,
Seeburg PH
.
Abstract
A complementary DNA (cDNA) encoding a serotonin receptor with 51% sequence identity to the 5HT-1C subtype was isolated from a rat brain cDNA library by homology screening. Transient expression of the cloned cDNA in mammalian cells was used to establish the pharmacological profile of the encoded receptor polypeptide. Membranes from transfected cells showed high-affinity binding of the serotonin antagonists spiperone, ketanserin and mianserin, low affinity for haloperidol (a dopamine D2 receptor antagonist), 8-OH-DPAT as well as MDL-72222 and no detectable binding of [3H]serotonin. This profile is consonant with the 5HT-2 subtype of serotonin receptors. In agreement with this assignment, serotonin increased the intracellular Ca2+ concentration and activated phosphoinositide hydrolysis in transfected mammalian cells. The agonist also elicited a current flow, blocked by spiperone, in Xenopus oocytes injected with in vitro synthesized RNA containing the cloned nucleotide sequences.
Bonner,
Cloning and expression of the human and rat m5 muscarinic acetylcholine receptor genes.
1988, Pubmed
Bonner,
Cloning and expression of the human and rat m5 muscarinic acetylcholine receptor genes.
1988,
Pubmed
Bonner,
Identification of a family of muscarinic acetylcholine receptor genes.
1987,
Pubmed
Bradley,
Proposals for the classification and nomenclature of functional receptors for 5-hydroxytryptamine.
1986,
Pubmed
Braun,
A novel subtype of muscarinic receptor identified by homology screening.
1987,
Pubmed
Chen,
High-efficiency transformation of mammalian cells by plasmid DNA.
1987,
Pubmed
Dascal,
Xenopus oocyte resting potential, muscarinic responses and the role of calcium and guanosine 3',5'-cyclic monophosphate.
1984,
Pubmed
,
Xenbase
Dixon,
Cloning of the gene and cDNA for mammalian beta-adrenergic receptor and homology with rhodopsin.
,
Pubmed
Fargin,
The genomic clone G-21 which resembles a beta-adrenergic receptor sequence encodes the 5-HT1A receptor.
1988,
Pubmed
Glennon,
Evidence for 5-HT2 involvement in the mechanism of action of hallucinogenic agents.
1984,
Pubmed
Gorman,
High efficiency DNA-mediated transformation of primate cells.
1983,
Pubmed
Grynkiewicz,
A new generation of Ca2+ indicators with greatly improved fluorescence properties.
1985,
Pubmed
Hoyer,
Molecular pharmacology and biology of 5-HT1C receptors.
1988,
Pubmed
Hoyer,
Molecular pharmacology of 5-HT1 and 5-HT2 recognition sites in rat and pig brain membranes: radioligand binding studies with [3H]5-HT, [3H]8-OH-DPAT, (-)[125I]iodocyanopindolol, [3H]mesulergine and [3H]ketanserin.
1985,
Pubmed
Hoyer,
Identification of serotonin 5-HT3 recognition sites in membranes of N1E-115 neuroblastoma cells by radioligand binding.
1988,
Pubmed
Julius,
Molecular characterization of a functional cDNA encoding the serotonin 1c receptor.
1988,
Pubmed
,
Xenbase
Kendall,
5-Hydroxytryptamine-stimulated inositol phospholipid hydrolysis in rat cerebral cortex slices: pharmacological characterization and effects of antidepressants.
1985,
Pubmed
Kobilka,
An intronless gene encoding a potential member of the family of receptors coupled to guanine nucleotide regulatory proteins.
,
Pubmed
Leysen,
Receptor binding profile of R 41 468, a novel antagonist at 5-HT2 receptors.
1981,
Pubmed
Leysen,
[3H]Ketanserin (R 41 468), a selective 3H-ligand for serotonin2 receptor binding sites. Binding properties, brain distribution, and functional role.
1982,
Pubmed
Leysen,
Serotonin-S2 receptor binding sites and functional correlates.
1984,
Pubmed
Lübbert,
Rat brain 5-HT1C receptors are encoded by a 5-6 kbase mRNA size class and are functionally expressed in injected Xenopus oocytes.
1987,
Pubmed
,
Xenbase
Lübbert,
cDNA cloning of a serotonin 5-HT1C receptor by electrophysiological assays of mRNA-injected Xenopus oocytes.
1987,
Pubmed
,
Xenbase
McIntosh,
Ultrasonic vocalisations facilitate sexual behaviour of female rats.
1978,
Pubmed
Nomura,
Inositol phosphate formation and chloride current responses induced by acetylcholine and serotonin through GTP-binding proteins in Xenopus oocyte after injection of rat brain messenger RNA.
1987,
Pubmed
,
Xenbase
Pazos,
Quantitative autoradiographic mapping of serotonin receptors in the rat brain. II. Serotonin-2 receptors.
1985,
Pubmed
Peralta,
Differential regulation of PI hydrolysis and adenylyl cyclase by muscarinic receptor subtypes.
1988,
Pubmed
Peroutka,
Pharmacological differentiation and characterization of 5-HT1A, 5-HT1B, and 5-HT1C binding sites in rat frontal cortex.
1986,
Pubmed
Peroutka,
5-Hydroxytryptamine receptor subtypes.
1988,
Pubmed
Roth,
Aortic recognition sites for serotonin (5HT) are coupled to phospholipase C and modulate phosphatidylinositol turnover.
1984,
Pubmed
Sanger,
DNA sequencing with chain-terminating inhibitors.
1977,
Pubmed
Takahashi,
Rat brain serotonin receptors in Xenopus oocytes are coupled by intracellular calcium to endogenous channels.
1987,
Pubmed
,
Xenbase
de Chaffoy de Courcelles,
Evidence that phospholipid turnover is the signal transducing system coupled to serotonin-S2 receptor sites.
1985,
Pubmed
