XB-ART-36574
Science
2007 Oct 12;3185848:245-50. doi: 10.1126/science.1143609.
Show Gene links
Show Anatomy links
The Chlamydomonas genome reveals the evolution of key animal and plant functions.
Merchant SS
,
Prochnik SE
,
Vallon O
,
Harris EH
,
Karpowicz SJ
,
Witman GB
,
Terry A
,
Salamov A
,
Fritz-Laylin LK
,
Maréchal-Drouard L
,
Marshall WF
,
Qu LH
,
Nelson DR
,
Sanderfoot AA
,
Spalding MH
,
Kapitonov VV
,
Ren Q
,
Ferris P
,
Lindquist E
,
Shapiro H
,
Lucas SM
,
Grimwood J
,
Schmutz J
,
Cardol P
,
Cerutti H
,
Chanfreau G
,
Chen CL
,
Cognat V
,
Croft MT
,
Dent R
,
Dutcher S
,
Fernández E
,
Fukuzawa H
,
González-Ballester D
,
González-Halphen D
,
Hallmann A
,
Hanikenne M
,
Hippler M
,
Inwood W
,
Jabbari K
,
Kalanon M
,
Kuras R
,
Lefebvre PA
,
Lemaire SD
,
Lobanov AV
,
Lohr M
,
Manuell A
,
Meier I
,
Mets L
,
Mittag M
,
Mittelmeier T
,
Moroney JV
,
Moseley J
,
Napoli C
,
Nedelcu AM
,
Niyogi K
,
Novoselov SV
,
Paulsen IT
,
Pazour G
,
Purton S
,
Ral JP
,
Riaño-Pachón DM
,
Riekhof W
,
Rymarquis L
,
Schroda M
,
Stern D
,
Umen J
,
Willows R
,
Wilson N
,
Zimmer SL
,
Allmer J
,
Balk J
,
Bisova K
,
Chen CJ
,
Elias M
,
Gendler K
,
Hauser C
,
Lamb MR
,
Ledford H
,
Long JC
,
Minagawa J
,
Page MD
,
Pan J
,
Pootakham W
,
Roje S
,
Rose A
,
Stahlberg E
,
Terauchi AM
,
Yang P
,
Ball S
,
Bowler C
,
Dieckmann CL
,
Gladyshev VN
,
Green P
,
Jorgensen R
,
Mayfield S
,
Mueller-Roeber B
,
Rajamani S
,
Sayre RT
,
Brokstein P
,
Dubchak I
,
Goodstein D
,
Hornick L
,
Huang YW
,
Jhaveri J
,
Luo Y
,
Martínez D
,
Ngau WC
,
Otillar B
,
Poliakov A
,
Porter A
,
Szajkowski L
,
Werner G
,
Zhou K
,
Grigoriev IV
,
Rokhsar DS
,
Grossman AR
.
Abstract
Chlamydomonas reinhardtii is a unicellular green alga whose lineage diverged from land plants over 1 billion years ago. It is a model system for studying chloroplast-based photosynthesis, as well as the structure, assembly, and function of eukaryotic flagella (cilia), which were inherited from the common ancestor of plants and animals, but lost in land plants. We sequenced the approximately 120-megabase nuclear genome of Chlamydomonas and performed comparative phylogenomic analyses, identifying genes encoding uncharacterized proteins that are likely associated with the function and biogenesis of chloroplasts or eukaryotic flagella. Analyses of the Chlamydomonas genome advance our understanding of the ancestral eukaryotic cell, reveal previously unknown genes associated with photosynthetic and flagellar functions, and establish links between ciliopathy and the composition and function of flagella.
PubMed ID: 17932292
PMC ID: PMC2875087
Article link: Science
References [+] :
Ansley,
Basal body dysfunction is a likely cause of pleiotropic Bardet-Biedl syndrome.
2003, Pubmed
Ansley, Basal body dysfunction is a likely cause of pleiotropic Bardet-Biedl syndrome. 2003, Pubmed
Armbrust, The genome of the diatom Thalassiosira pseudonana: ecology, evolution, and metabolism. 2004, Pubmed
Avidor-Reiss, Decoding cilia function: defining specialized genes required for compartmentalized cilia biogenesis. 2004, Pubmed
Bhattacharya, Photosynthetic eukaryotes unite: endosymbiosis connects the dots. 2004, Pubmed
Blacque, Loss of C. elegans BBS-7 and BBS-8 protein function results in cilia defects and compromised intraflagellar transport. 2004, Pubmed
Briggs, More than one way to build a flagellum: comparative genomics of parasitic protozoa. 2004, Pubmed
Ciccarelli, Toward automatic reconstruction of a highly resolved tree of life. 2006, Pubmed
Do, A defect in coenzyme Q biosynthesis is responsible for the respiratory deficiency in Saccharomyces cerevisiae abc1 mutants. 2001, Pubmed
Duchêne, Dual targeting is the rule for organellar aminoacyl-tRNA synthetases in Arabidopsis thaliana. 2005, Pubmed
Eichinger, The genome of the social amoeba Dictyostelium discoideum. 2005, Pubmed
Gaillard, Disruption of the A-kinase anchoring domain in flagellar radial spoke protein 3 results in unregulated axonemal cAMP-dependent protein kinase activity and abnormal flagellar motility. 2006, Pubmed
Ghirardi, Hydrogenases and hydrogen photoproduction in oxygenic photosynthetic organisms. 2007, Pubmed
González-Ballester, Functional genomics of the regulation of the nitrate assimilation pathway in Chlamydomonas. 2005, Pubmed
Gray, Evolution of organellar genomes. 1999, Pubmed
Grossman, Novel metabolism in Chlamydomonas through the lens of genomics. 2007, Pubmed
Grossman, MACRONUTRIENT UTILIZATION BY PHOTOSYNTHETIC EUKARYOTES AND THE FABRIC OF INTERACTIONS. 2001, Pubmed
Harris, CHLAMYDOMONAS AS A MODEL ORGANISM. 2001, Pubmed
Hasegawa, Stimulation of in vitro motility of Chlamydomonas axonemes by inhibition of cAMP-dependent phosphorylation. 1987, Pubmed
Jain, EST assembly supported by a draft genome sequence: an analysis of the Chlamydomonas reinhardtii transcriptome. 2007, Pubmed
Kathir, Molecular map of the Chlamydomonas reinhardtii nuclear genome. 2003, Pubmed
Keeling, A brief history of plastids and their hosts. 2004, Pubmed
Keeling, The tree of eukaryotes. 2005, Pubmed
Keller, Proteomic analysis of isolated chlamydomonas centrioles reveals orthologs of ciliary-disease genes. 2005, Pubmed
Lamb, Eyespot-assembly mutants in Chlamydomonas reinhardtii. 1999, Pubmed
Li, Comparative genomics identifies a flagellar and basal body proteome that includes the BBS5 human disease gene. 2004, Pubmed
Manton, Observations on the fine structure and development of the spindle at mitosis and meiosis in a marine centric diatom (Lithodesmium undulatum). 3. The later stages of meiosis I in male gametogenesis. 1970, Pubmed
O'Toole, Three-dimensional organization of basal bodies from wild-type and delta-tubulin deletion strains of Chlamydomonas reinhardtii. 2003, Pubmed
Ou, Sensory ciliogenesis in Caenorhabditis elegans: assignment of IFT components into distinct modules based on transport and phenotypic profiles. 2007, Pubmed
Palenik, The tiny eukaryote Ostreococcus provides genomic insights into the paradox of plankton speciation. 2007, Pubmed
Pasquale, Cyclic AMP functions as a primary sexual signal in gametes of Chlamydomonas reinhardtii. 1987, Pubmed
Pazour, The DHC1b (DHC2) isoform of cytoplasmic dynein is required for flagellar assembly. 1999, Pubmed
Pazour, Proteomic analysis of a eukaryotic cilium. 2005, Pubmed
Pazour, Identification of predicted human outer dynein arm genes: candidates for primary ciliary dyskinesia genes. 2006, Pubmed
Perry, Convergent evolution of receptors for protein import into mitochondria. 2006, Pubmed
Pollock, Insights into the acclimation of Chlamydomonas reinhardtii to sulfur deprivation. 2005, Pubmed
Qin, Intraflagellar transport (IFT) cargo: IFT transports flagellar precursors to the tip and turnover products to the cell body. 2004, Pubmed
Rosenbaum, Intraflagellar transport. 2002, Pubmed
Rymarquis, Beyond complementation. Map-based cloning in Chlamydomonas reinhardtii. 2005, Pubmed
Sato, SOUL in mouse eyes is a new hexameric heme-binding protein with characteristic optical absorption, resonance Raman spectral, and heme-binding properties. 2004, Pubmed
Schaap, Guanylyl cyclases across the tree of life. 2005, Pubmed
Schmidt, Proteomic analysis of the eyespot of Chlamydomonas reinhardtii provides novel insights into its components and tactic movements. 2006, Pubmed
Somlo, Human disease: calcium signaling in polycystic kidney disease. 2001, Pubmed
Storms, A fine structure analysis of meiotic pairing in Chlamydomonas reinhardi. 1977, Pubmed
Vidi, Tocopherol cyclase (VTE1) localization and vitamin E accumulation in chloroplast plastoglobule lipoprotein particles. 2006, Pubmed
Vílchez, Studies on the suitability of alginate-entrapped Chlamydomonas reinhardtii cells for sustaining nitrate consumption processes. 2001, Pubmed
Yandell, Large-scale trends in the evolution of gene structures within 11 animal genomes. 2006, Pubmed
Yoon, A molecular timeline for the origin of photosynthetic eukaryotes. 2004, Pubmed
Ytterberg, Protein profiling of plastoglobules in chloroplasts and chromoplasts. A surprising site for differential accumulation of metabolic enzymes. 2006, Pubmed
Yuan, The institute for genomic research Osa1 rice genome annotation database. 2005, Pubmed
Zimmermann, GENEVESTIGATOR. Arabidopsis microarray database and analysis toolbox. 2004, Pubmed
Zylka, Discovery of a putative heme-binding protein family (SOUL/HBP) by two-tissue suppression subtractive hybridization and database searches. 1999, Pubmed