XB-ART-22601
Cell
1993 May 07;733:571-83.
Show Gene links
Show Anatomy links
Raf-1 kinase is essential for early Xenopus development and mediates the induction of mesoderm by FGF.
???displayArticle.abstract???
Animal cap explants from Xenopus embryos injected with a dominant negative Raf-1 mutant, termed NAF (not a functional Raf), demonstrated a complete block to basic fibroblast growth factor (FGF)-stimulated mesoderm induction. Activin induction of mesoderm was normal in embryos that expressed NAF. Injection of NAF RNA into 2-cell stage embryos blocked normal development during neurula stages and caused severe posterior truncations in tadpoles. The phenotype induced by NAF could be rescued by coinjection of wild-type raf-1 RNA. The NAF mutant functioned by specifically blocking the activation of endogenous Raf kinase activity. These findings suggest that Raf-1 mediates FGF, but not activin, receptor signaling during mesoderm induction and implicate Raf-1 as a key signaling molecule in the development of posterior structure.
???displayArticle.pubmedLink??? 7683975
???displayArticle.link??? Cell
???displayArticle.grants???
Species referenced: Xenopus
Genes referenced: raf1