Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
Mol Biol Rep
1979 May 31;51-2:59-64. doi: 10.1007/bf00777489.
Show Gene links
Show Anatomy links
Free cytoplasmic messenger ribonucleoprotein complexes from rabbit reticulocytes.
Princen HM
,
van Eekelen CA
,
Asselbergs FA
,
van Venrooij WJ
.
Abstract
Free cytoplasmic globin mRNA containing mRNP-particles were isolated from rabbit reticulocytes by zonal sucrose gradient centrifugation and their properties were compared with mRNP particles isolated in the same way from EDTA-dissociated reticulocyte polyribosomes. The average poly(A)-length of 9S mRNA from free cytoplasmic mRNP was 17-20 nucleotides being about two times shorter than the average poly(A)-length of polysomal 9S mRNA. The protein composition of the free cytoplasmic mRNP particles disclosed the absence of the 76,000 dalton protein which is associated with the 3'poly(A)-segment of polysomal globin mRNA. It was concluded that free cytoplasmic mRNP-particles from rabbit reticulocytes can be classified as "old" mRNP in a post-translational phase. Free cytoplasmic mRNPs were translated in heterologous cell-free systems as well as in Xenopus laevis oocytes. Addition of hemin stimulated the synthesis of alpha-globin in all systems, while the presence of the cap analogue m7G(5')p inhibited translation of free cytoplasmic mRNA completely. The latter finding suggested that free cytoplasmic mRNA has a 5' terminal "cap". Shortening of the poly(A)-segment with concomitant loss of the 76,000 dalton protein may lead to less efficient translation of free cytoplasmic mRNP.
Asselbergs,
Synthesis of lens crystallins in Xenopus oocytes as determined by quantitative immunoprecipitation.
1978, Pubmed,
Xenbase
Asselbergs,
Synthesis of lens crystallins in Xenopus oocytes as determined by quantitative immunoprecipitation.
1978,
Pubmed
,
Xenbase
Blobel,
A protein of molecular weight 78,000 bound to the polyadenylate region of eukaryotic messenger RNAs.
1973,
Pubmed
Bonanou-Tzedaki,
Presence of haemoglobin messenger RNA in the postribosomal supernatant of rabbit reticulocytes and conditions necessary for its translation.
1972,
Pubmed
Brawerman,
Alterations in the size of the poly(a) segment in newly-synthesized messenger RNA of mouse sarcoma 180 ascites cells.
1973,
Pubmed
Civelli,
Evidence for a translational inhibitor linked to globin mRNA in untranslated free cytoplasmic messenger ribonucleoprotein complexes.
1976,
Pubmed
Dworkin,
Relationship between the mRNA of polysomes and free ribonucleoprotein particles in the early sea urchin embryo.
1976,
Pubmed
Efstratiadis,
The primary structure of rabbit beta-globin mRNA as determined from cloned DNA.
1977,
Pubmed
Gianni,
Globin -chain synthesis directed by "supernatant" 10S RNA from rabbit reticulocytes.
1972,
Pubmed
Giglioni,
Translational control of globin synthesis by haemin in Xenopus oocytes.
1973,
Pubmed
,
Xenbase
Gorski,
Poly(A) size class distribution in globin mRNAs as a function of time.
1975,
Pubmed
Gross,
Isolation of two initiation factors that can partially reverse the inhibition of protein synthesis due to hemin deficiency or the hemin-controlled translational represssor in rabbit reticulocyte lysates.
1977,
Pubmed
Hickey,
Inhibition of initiation of protein synthesis by 7-methylguanosine-5'-monophosphate.
1976,
Pubmed
Huez,
Readenylation of polyadenylate-free globin messenger RNA restores its stability in vivo.
1975,
Pubmed
,
Xenbase
Hunt,
The nature of the 5'-linked 5' nucleotide sequence at the 5' end of rabbit globin messenger ribonucleic acid.
1976,
Pubmed
Hunt,
Control of haemoglobin synthesis: a difference in the size of the polysomes making alpha and beta chains.
1968,
Pubmed
Huynh-Van-Tan,
Isolation and characterization of free cytoplasmic messenger ribonucleoproteins from rabbit reticulocyte.
1978,
Pubmed
Jacobs-Lorena,
Messenger RNA for globin in the postribosomal supernatant of rabbit reticulocytes.
1972,
Pubmed
Janssen,
Characterization of poly(A)-protein complexes isolated from free and membrane-bound polyribosomes of Ehrlich ascites tumor cells.
1976,
Pubmed
Laemmli,
Cleavage of structural proteins during the assembly of the head of bacteriophage T4.
1970,
Pubmed
Lodish,
Model for the regulation of mRNA translation applied to haemoglobin synthesis.
1974,
Pubmed
Lodish,
Regulation of hemoglobin synthesis. Equal rates of translation and termination of - and -globin chains.
1972,
Pubmed
London,
The role of hemin in the regulation of protein synthesis in erythroid cells.
1976,
Pubmed
Marbaix,
Evolution of the polyribosome distribution during in vivo reticulocyte maturation.
1970,
Pubmed
Marbaix,
Free cytoplasmic alpha-globin messenger RNA appears during the maturation of rabbit reticulocytes.
1976,
Pubmed
Miller,
Isolation of a protein fraction from reticulocyte ribosomes required for de novo synthesis of hemoglobin.
1968,
Pubmed
Muthukrishnan,
5'-Terminal 7-methylguanosine in eukaryotic mRNA is required for translation.
1975,
Pubmed
Nokin,
Molecular modifications associated with Aging of globin messenger RNA in vitro.
1976,
Pubmed
Pelham,
An efficient mRNA-dependent translation system from reticulocyte lysates.
1976,
Pubmed
Perry,
The methylated constituents of globin mRNA.
1975,
Pubmed
Phillips,
Ratios of alpha-to beta-globin mRNA and regulation of globin synthesis in reticulocytes.
1977,
Pubmed
Schwartz,
The association of protein with the polyadenylic acid of HeLa cell messenger RNA: evidence for a "transport" role of a 75,000 molecular weight polypeptide.
1976,
Pubmed
Sen,
Inhibition of reovirus messenger RNA methylation in extracts of interferon-treated Ehrlich ascites tumor cells.
1975,
Pubmed
Shafritz,
Evidence for role of m7G5'-phosphate group in recognition of eukaryotic mRNA by initiation factor IF-M3.
1976,
Pubmed
Shatkin,
Capping of eucaryotic mRNAs.
1976,
Pubmed
Sheiness,
Polyadenylic acid segment in mRNA becomes shorter with age.
1973,
Pubmed
Sippel,
Translational properties of rabbit globin mRNA after specific removal of poly(A) with ribonuclease H.
1974,
Pubmed
Slegers,
Messenger ribonucleoprotein complexes of cryptobiotic embryos of Artemia salina.
1977,
Pubmed
Sonenshein,
Entry of mRNA into polyribosomes during recovery from starvation in mouse sarcoma 180 cells.
1977,
Pubmed
Soreq,
In vitro translation of polyadenylic acid-free rabbit globin messenger RNA.
1974,
Pubmed
Spirin,
The second Sir Hans Krebs Lecture. Informosomes.
1969,
Pubmed
Spirin,
Energetics of the ribosome.
1978,
Pubmed
Yap,
Effect of a short term fast on the distribution of cytoplasmic albumin messenger ribonucleic acid in rat liver. Evidence for formation of free albumin messenger ribonucleoprotein particles.
1978,
Pubmed
van Venrooij,
Specific poly-A-binding protein of 76,000 molecular weight in polyribosomes is not present on poly A of free cytoplasmic mRNP.
1977,
Pubmed