XB-ART-29743
J Immunol
1984 May 01;1325:2165-7.
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Phorbol ester increases the level of interleukin 2 mRNA in mitogen-stimulated human lymphocytes.
Abstract
TPA alone did not induce the production of IL 2 in human tonsillar lymphocytes but enhanced the PHA-induced IL 2 production by seven-fold. That the effect of TPA was due to an increase in IL 2 mRNA was demonstrated by examining the amount of IL 2 mRNA translatable in Xenopus laevis oocytes, and by Northern blotting analysis using IL 2 cDNA as a probe. In these ways, it was shown that TPA alone did not induce any significant IL 2 mRNA synthesis, but when added together with PHA it increased the level of IL 2 mRNA by at least 10-fold, as compared with that induced by PHA alone.
PubMed ID: 6609189
Article link: J Immunol