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Human and Xenopus cDNAs encoding budding yeast Cdc7-related kinases: in vitro phosphorylation of MCM subunits by a putative human homologue of Cdc7.
Sato N
,
Arai K
,
Masai H
.
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Saccharomyces cerevisiae Cdc7 kinase is essential for initiation of DNA replication, and Hsk1, a related kinase of Schizosaccharomyces pombe, is also required for DNA replication of fission yeast cells. We report here cDNAs encoding Cdc7-related kinases from human and Xenopus (huCdc7 and xeCdc7, respectively). The cloned cDNA for huCdc7 contains an open reading frame consisting of 574 amino acids with a predicted molecular weight of 63,847 that possesses overall amino acid identity of 32% (54% including similar residues) to Cdc7 and Hsk1. huCDC7 is transcribed in the various tissues examined, but most abundantly in testis. Three transcripts of 4.4, 3.5 and 2.4 kb in length are detected. The 3.5 kb transcript is the most predominant and is expressed in all the tissues examined. A cDNA containing a 91 nucleotide insertion at the N-terminal region of huCDC7 is also detected, suggesting the presence of multiple splicing variants. The huCdc7 protein is expressed at a constant level during the mitotic cell cycle and is localized primarily in nuclei in interphase and distributed diffusibly in cytoplasm in the mitotic phase. The wild-type huCdc7 protein expressed in COS7 cells phosphorylates MCM2 and MCM3 proteins in vitro, suggesting that huCdc7 may regulate processes of DNA replication by modulating MCM functions.
Bell,
ATP-dependent recognition of eukaryotic origins of DNA replication by a multiprotein complex.
1992, Pubmed
Bell,
ATP-dependent recognition of eukaryotic origins of DNA replication by a multiprotein complex.
1992,
Pubmed
Blow,
A role for the nuclear envelope in controlling DNA replication within the cell cycle.
1988,
Pubmed
,
Xenbase
Carpenter,
Role for a Xenopus Orc2-related protein in controlling DNA replication.
1996,
Pubmed
,
Xenbase
Cocker,
An essential role for the Cdc6 protein in forming the pre-replicative complexes of budding yeast.
1996,
Pubmed
Coué,
Chromatin binding, nuclear localization and phosphorylation of Xenopus cdc21 are cell-cycle dependent and associated with the control of initiation of DNA replication.
1996,
Pubmed
,
Xenbase
Diffley,
Protein-DNA interactions at a yeast replication origin.
1992,
Pubmed
Donovan,
Replication origins in eukaroytes.
1996,
Pubmed
,
Xenbase
Dowell,
Interaction of Dbf4, the Cdc7 protein kinase regulatory subunit, with yeast replication origins in vivo.
1994,
Pubmed
Ehrenhofer-Murray,
Separation of origin recognition complex functions by cross-species complementation.
1995,
Pubmed
Fey,
Epithelial cytoskeletal framework and nuclear matrix-intermediate filament scaffold: three-dimensional organization and protein composition.
1984,
Pubmed
Fox,
The origin recognition complex has essential functions in transcriptional silencing and chromosomal replication.
1995,
Pubmed
Gavin,
Conserved initiator proteins in eukaryotes.
1995,
Pubmed
Gossen,
A Drosophila homolog of the yeast origin recognition complex.
1995,
Pubmed
Hamlin,
On the nature of replication origins in higher eukaryotes.
1995,
Pubmed
Hanks,
The protein kinase family: conserved features and deduced phylogeny of the catalytic domains.
1988,
Pubmed
Hartwell,
Saccharomyces cerevisiae cell cycle.
1974,
Pubmed
Hu,
The P1 family: a new class of nuclear mammalian proteins related to the yeast Mcm replication proteins.
1993,
Pubmed
Huberman,
Prokaryotic and eukaryotic replicons.
1995,
Pubmed
Ikeda,
A fusion protein library: an improved method for rapid screening and characterization of DNA binding or interacting proteins.
1996,
Pubmed
Jackson,
Cell cycle regulation of the yeast Cdc7 protein kinase by association with the Dbf4 protein.
1993,
Pubmed
Kimura,
Molecular cloning of cDNA encoding mouse Cdc21 and CDC46 homologs and characterization of the products: physical interaction between P1(MCM3) and CDC46 proteins.
1995,
Pubmed
Kitada,
Temperature-sensitive cdc7 mutations of Saccharomyces cerevisiae are suppressed by the DBF4 gene, which is required for the G1/S cell cycle transition.
1992,
Pubmed
Kubota,
Identification of the yeast MCM3-related protein as a component of Xenopus DNA replication licensing factor.
1995,
Pubmed
,
Xenbase
Liang,
ORC and Cdc6p interact and determine the frequency of initiation of DNA replication in the genome.
1995,
Pubmed
Loo,
The origin recognition complex in silencing, cell cycle progression, and DNA replication.
1995,
Pubmed
Marahrens,
A yeast chromosomal origin of DNA replication defined by multiple functional elements.
1992,
Pubmed
Marchuk,
Construction of T-vectors, a rapid and general system for direct cloning of unmodified PCR products.
1991,
Pubmed
Masai,
hsk1+, a Schizosaccharomyces pombe gene related to Saccharomyces cerevisiae CDC7, is required for chromosomal replication.
1995,
Pubmed
Patterson,
Molecular characterization of cell cycle gene CDC7 from Saccharomyces cerevisiae.
1986,
Pubmed
Rao,
Functional conservation of multiple elements in yeast chromosomal replicators.
1994,
Pubmed
Rao,
The origin recognition complex interacts with a bipartite DNA binding site within yeast replicators.
1995,
Pubmed
Rowley,
Recent developments in the initiation of chromosomal DNA replication: a complex picture emerges.
1994,
Pubmed
Rowley,
Initiation complex assembly at budding yeast replication origins begins with the recognition of a bipartite sequence by limiting amounts of the initiator, ORC.
1995,
Pubmed
Sclafani,
Differential regulation of the yeast CDC7 gene during mitosis and meiosis.
1988,
Pubmed
Sclafani,
Cdc7 protein kinase for DNA metabolism comes of age.
1994,
Pubmed
Theis,
Domain B of ARS307 contains two functional elements and contributes to chromosomal replication origin function.
1994,
Pubmed
Thömmes,
Properties of the nuclear P1 protein, a mammalian homologue of the yeast Mcm3 replication protein.
1992,
Pubmed
Todorov,
BM28, a human member of the MCM2-3-5 family, is displaced from chromatin during DNA replication.
1995,
Pubmed
Treisman,
Cell proliferation and DNA replication defects in a Drosophila MCM2 mutant.
1995,
Pubmed
Walker,
Analysis of the interactions of functional domains of a nuclear origin of replication from Saccharomyces cerevisiae.
1991,
Pubmed
Yan,
Cell cycle-regulated nuclear localization of MCM2 and MCM3, which are required for the initiation of DNA synthesis at chromosomal replication origins in yeast.
1993,
Pubmed
Yoon,
Regulation of Saccharomyces cerevisiae CDC7 function during the cell cycle.
1993,
Pubmed
