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Synthesis and characterization of a DNA complementary to Xenopus laevis albumin mRNA.
Dimitriadis GJ
,
Zongza-Dimitriadis V
.
Abstract
Albumin complementary DNA (cDNA) was transcribed from purified albumin mRNA from the liver of Xenopus laevis. The resultant cDNA was an almost full length copy as defined by denaturing gel electrophoresis; was hybridized specifically to albumin mRNA with pseudo-first-order reaction kinetics and the mRNA . cDNA hybrid exhibited a sharp melting profile with a Tm of 83 degrees C. The identity of the cDNA was confirmed by gel electrophoresis following hybridization-arrested translation.
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