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Inhibiting MAP kinase activity prevents calcium transients and mitosis entry in early sea urchin embryos.
Philipova R
,
Larman MG
,
Leckie CP
,
Harrison PK
,
Groigno L
,
Whitaker M
.
Abstract
A transient calcium increase triggers nuclear envelope breakdown (mitosis entry) in sea urchin embryos. Cdk1/cyclin B kinase activation is also known to be required for mitosis entry. More recently, MAP kinase activity has also been shown to increase during mitosis. In sea urchin embryos, both kinases show a similar activation profile, peaking at the time of mitosis entry. We tested whether the activity of both kinases is required for mitosis entry and whether either kinase controls mitotic calcium signals. We found that reducing the activity of either mitotic kinase prevents nuclear envelope breakdown, despite the presence of a calcium transient, when cdk1/cyclin B kinase activity is alone inhibited. When MAP kinase activity alone was inhibited, the calcium signal was absent, suggesting that MAP kinase activity is required to generate the calcium transient that triggers nuclear envelope breakdown. However, increasing intracellular free calcium by microinjection of calcium buffers or InsP(3) while MAP kinase was inhibited did not itself induce nuclear envelope breakdown, indicating that additional MAP kinase-regulated events are necessary. After MAP kinase inhibition early in the cell cycle, the early events of the cell cycle (pronuclear migration/fusion and DNA synthesis) were unaffected, but chromosome condensation and spindle assembly are prevented. These data indicate that in sea urchin embryos, MAP kinase activity is part of a signaling complex alongside two components previously shown to be essential for entry into mitosis: the calcium transient and the increase in cdk1/cyclinB kinase activity.
Alessi,
The human CL100 gene encodes a Tyr/Thr-protein phosphatase which potently and specifically inactivates MAP kinase and suppresses its activation by oncogenic ras in Xenopus oocyte extracts.
1993, Pubmed,
Xenbase
Alessi,
The human CL100 gene encodes a Tyr/Thr-protein phosphatase which potently and specifically inactivates MAP kinase and suppresses its activation by oncogenic ras in Xenopus oocyte extracts.
1993,
Pubmed
,
Xenbase
Alessi,
The cyclin-dependent kinase inhibitors olomoucine and roscovitine arrest human fibroblasts in G1 phase by specific inhibition of CDK2 kinase activity.
1998,
Pubmed
Baitinger,
Multifunctional Ca2+/calmodulin-dependent protein kinase is necessary for nuclear envelope breakdown.
1990,
Pubmed
Becchetti,
Lithium blocks cell cycle transitions in the first cell cycles of sea urchin embryos, an effect rescued by myo-inositol.
1997,
Pubmed
Belmont,
Identification of a protein that interacts with tubulin dimers and increases the catastrophe rate of microtubules.
1996,
Pubmed
,
Xenbase
Berridge,
Calcium signalling and cell proliferation.
1995,
Pubmed
Bradbury,
Reversible histone modifications and the chromosome cell cycle.
1992,
Pubmed
Brattsand,
Cell-cycle-regulated phosphorylation of oncoprotein 18 on Ser16, Ser25 and Ser38.
1994,
Pubmed
Carroll,
The relationship between calcium, MAP kinase, and DNA synthesis in the sea urchin egg at fertilization.
2000,
Pubmed
Cheung,
Synergistic coupling of histone H3 phosphorylation and acetylation in response to epidermal growth factor stimulation.
2000,
Pubmed
Chiri,
Evidence for MAP kinase activation during mitotic division.
1998,
Pubmed
Ciapa,
Cell-cycle calcium transients driven by cyclic changes in inositol trisphosphate levels.
1994,
Pubmed
DeSilva,
Inhibition of mitogen-activated protein kinase kinase blocks T cell proliferation but does not induce or prevent anergy.
1998,
Pubmed
Downes,
A topoisomerase II-dependent G2 cycle checkpoint in mammalian cells/.
1994,
Pubmed
,
Xenbase
Drechsel,
Modulation of the dynamic instability of tubulin assembly by the microtubule-associated protein tau.
1992,
Pubmed
Ducommun,
cdc2 phosphorylation is required for its interaction with cyclin.
1991,
Pubmed
,
Xenbase
Dunphy,
The decision to enter mitosis.
1994,
Pubmed
Dunphy,
The cdc25 protein contains an intrinsic phosphatase activity.
1991,
Pubmed
Dunphy,
The Xenopus cdc2 protein is a component of MPF, a cytoplasmic regulator of mitosis.
1988,
Pubmed
,
Xenbase
Edelmann,
Meiotic pachytene arrest in MLH1-deficient mice.
1996,
Pubmed
Edgecombe,
A cyclin-abundance cycle-independent p34cdc2 tyrosine phosphorylation cycle in early sea urchin embryos.
1991,
Pubmed
Favata,
Identification of a novel inhibitor of mitogen-activated protein kinase kinase.
1998,
Pubmed
Ferrell,
Cell cycle tyrosine phosphorylation of p34cdc2 and a microtubule-associated protein kinase homolog in Xenopus oocytes and eggs.
1991,
Pubmed
,
Xenbase
Ferrell,
The biochemical basis of an all-or-none cell fate switch in Xenopus oocytes.
1998,
Pubmed
,
Xenbase
Gautier,
Cyclin is a component of maturation-promoting factor from Xenopus.
1990,
Pubmed
,
Xenbase
Gautier,
cdc25 is a specific tyrosine phosphatase that directly activates p34cdc2.
1991,
Pubmed
,
Xenbase
Genevière-Garrigues,
Active cyclin B-cdc2 kinase does not inhibit DNA replication and cannot drive prematurely fertilized sea urchin eggs into mitosis.
1995,
Pubmed
Gotoh,
In vitro effects on microtubule dynamics of purified Xenopus M phase-activated MAP kinase.
1991,
Pubmed
,
Xenbase
Groigno,
An anaphase calcium signal controls chromosome disjunction in early sea urchin embryos.
1998,
Pubmed
Guadagno,
Requirement for MAPK activation for normal mitotic progression in Xenopus egg extracts.
1998,
Pubmed
,
Xenbase
Guo,
Chromosome condensation induced by fostriecin does not require p34cdc2 kinase activity and histone H1 hyperphosphorylation, but is associated with enhanced histone H2A and H3 phosphorylation.
1995,
Pubmed
Hagting,
MPF localization is controlled by nuclear export.
1998,
Pubmed
Hepler,
The role of calcium in cell division.
1994,
Pubmed
Hoshi,
Mitogen-activated-protein-kinase-catalyzed phosphorylation of microtubule-associated proteins, microtubule-associated protein 2 and microtubule-associated protein 4, induces an alteration in their function.
1992,
Pubmed
Kao,
Active involvement of Ca2+ in mitotic progression of Swiss 3T3 fibroblasts.
1990,
Pubmed
Kono,
A cell cycle-associated change in Ca2+ releasing activity leads to the generation of Ca2+ transients in mouse embryos during the first mitotic division.
1996,
Pubmed
Koshland,
Mitotic chromosome condensation.
1996,
Pubmed
Kumano,
Calcium-mediated inactivation of the MAP kinase pathway in sea urchin eggs at fertilization.
2001,
Pubmed
Labbé,
MPF from starfish oocytes at first meiotic metaphase is a heterodimer containing one molecule of cdc2 and one molecule of cyclin B.
1989,
Pubmed
Larsson,
G2/M transition requires multisite phosphorylation of oncoprotein 18 by two distinct protein kinase systems.
1995,
Pubmed
Lew,
Regulatory roles of cyclin dependent kinase phosphorylation in cell cycle control.
1996,
Pubmed
Lewis,
XCL100, an inducible nuclear MAP kinase phosphatase from Xenopus laevis: its role in MAP kinase inactivation in differentiated cells and its expression during early development.
1995,
Pubmed
,
Xenbase
Marklund,
Multiple signal transduction pathways induce phosphorylation of serines 16, 25, and 38 of oncoprotein 18 in T lymphocytes.
1993,
Pubmed
Meijer,
Biochemical and cellular effects of roscovitine, a potent and selective inhibitor of the cyclin-dependent kinases cdc2, cdk2 and cdk5.
1997,
Pubmed
,
Xenbase
Moudrianakis,
Structure of the histone octamer core of the nucleosome and its potential interactions with DNA.
1993,
Pubmed
Murray,
Cyclin synthesis drives the early embryonic cell cycle.
1989,
Pubmed
,
Xenbase
Newport,
Disassembly of the nucleus in mitotic extracts: membrane vesicularization, lamin disassembly, and chromosome condensation are independent processes.
1987,
Pubmed
,
Xenbase
Nigg,
Assembly-disassembly of the nuclear lamina.
1992,
Pubmed
Nishioka,
Relationships between DNA synthesis and mitotic events in fertilized sea urchin eggs: aphidicolin inhibits DNA synthesis, nuclear breakdown and proliferation of microtubule organizing centers, but not cycles of microtubule assembly.
1984,
Pubmed
Olsen,
KT5720 and U-98017 inhibit MAPK and alter the cytoskeleton and cell morphology.
1998,
Pubmed
Patel,
Calcium/calmodulin-dependent phosphorylation and activation of human Cdc25-C at the G2/M phase transition in HeLa cells.
1999,
Pubmed
Peter,
Disassembly of in vitro formed lamin head-to-tail polymers by CDC2 kinase.
1991,
Pubmed
Peter,
In vitro disassembly of the nuclear lamina and M phase-specific phosphorylation of lamins by cdc2 kinase.
1990,
Pubmed
Philipova,
ERK1 activation is required for S-phase onset and cell cycle progression after fertilization in sea urchin embryos.
2005,
Pubmed
,
Xenbase
Philipova,
MAP kinase activity increases during mitosis in early sea urchin embryos.
1998,
Pubmed
Santella,
The role of calcium in the cell cycle: facts and hypotheses.
1998,
Pubmed
Sassone-Corsi,
Requirement of Rsk-2 for epidermal growth factor-activated phosphorylation of histone H3.
1999,
Pubmed
Schatten,
Taxol inhibits the nuclear movements during fertilization and induces asters in unfertilized sea urchin eggs.
1982,
Pubmed
Shapiro,
Extracellular signal-regulated kinase activates topoisomerase IIalpha through a mechanism independent of phosphorylation.
1999,
Pubmed
Shapiro,
Activation of the MKK/ERK pathway during somatic cell mitosis: direct interactions of active ERK with kinetochores and regulation of the mitotic 3F3/2 phosphoantigen.
1998,
Pubmed
Shearer,
Role of phospholipase Cgamma at fertilization and during mitosis in sea urchin eggs and embryos.
1999,
Pubmed
Shiina,
Regulation of a major microtubule-associated protein by MPF and MAP kinase.
1992,
Pubmed
,
Xenbase
Solomon,
Role of phosphorylation in p34cdc2 activation: identification of an activating kinase.
1992,
Pubmed
,
Xenbase
Steinhardt,
Intracellular free calcium rise triggers nuclear envelope breakdown in the sea urchin embryo.
1988,
Pubmed
Sun,
MKP-1 (3CH134), an immediate early gene product, is a dual specificity phosphatase that dephosphorylates MAP kinase in vivo.
1993,
Pubmed
Swann,
The part played by inositol trisphosphate and calcium in the propagation of the fertilization wave in sea urchin eggs.
1986,
Pubmed
Swanson,
An endogenous calcium oscillator may control early embryonic division.
1997,
Pubmed
,
Xenbase
Takizawa,
Control of mitosis by changes in the subcellular location of cyclin-B1-Cdk1 and Cdc25C.
2000,
Pubmed
,
Xenbase
Tamemoto,
Biphasic activation of two mitogen-activated protein kinases during the cell cycle in mammalian cells.
1992,
Pubmed
Tombes,
Meiosis, egg activation, and nuclear envelope breakdown are differentially reliant on Ca2+, whereas germinal vesicle breakdown is Ca2+ independent in the mouse oocyte.
1992,
Pubmed
Tombes,
Intracellular free calcium and mitosis in mammalian cells: anaphase onset is calcium modulated, but is not triggered by a brief transient.
1989,
Pubmed
Twigg,
Translational control of InsP3-induced chromatin condensation during the early cell cycles of sea urchin embryos.
1988,
Pubmed
Verlhac,
Microtubule and chromatin behavior follow MAP kinase activity but not MPF activity during meiosis in mouse oocytes.
1994,
Pubmed
,
Xenbase
Wang,
A role for mitogen-activated protein kinase in the spindle assembly checkpoint in XTC cells.
1997,
Pubmed
,
Xenbase
Whitaker,
Calcium and cell cycle control.
1990,
Pubmed
Whitaker,
Calcium and mitosis.
2001,
Pubmed
Wilding,
Local perinuclear calcium signals associated with mitosis-entry in early sea urchin embryos.
1996,
Pubmed
Xiong,
A positive-feedback-based bistable 'memory module' that governs a cell fate decision.
2003,
Pubmed
,
Xenbase
Yue,
Mos mediates the mitotic activation of p42 MAPK in Xenopus egg extracts.
2004,
Pubmed
,
Xenbase
