Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
Philos Trans R Soc Lond B Biol Sci
2008 Apr 12;3631495:1371-5. doi: 10.1098/rstb.2007.2254.
Show Gene links
Show Anatomy links
Calcium signalling during neural induction in Xenopus laevis embryos.
Moreau M
,
Néant I
,
Webb SE
,
Miller AL
,
Leclerc C
.
???displayArticle.abstract???
In Xenopus, experiments performed with isolated ectoderm suggest that neural determination is a 'by default' mechanism, which occurs when bone morphogenetic proteins (BMPs) are antagonized by extracellular antagonists, BMP being responsible for the determination of epidermis. However, Ca(2+) imaging of intact Xenopus embryos reveals patterns of Ca(2+) transients which are generated via the activation of dihydropyridine-sensitive Ca(2+) channels in the dorsal ectoderm but not in the ventral ectoderm. These increases in the concentration of intracellular Ca(2+)([Ca(2+)]i) appear to be necessary and sufficient to orient the ectodermal cells towards a neural fate as increasing the [Ca(2+)]i artificially results in neuralization of the ectoderm. We constructed a subtractive cDNA library between untreated and caffeine-treated ectoderms (to increase [Ca(2+)]i) and then identified early Ca(2+)-sensitive target genes expressed in the neural territories. One of these genes, an arginine methyltransferase, controls the expression of the early proneural gene, Zic3. Here, we discuss the evidence for the existence of an alternative model to the 'by default' mechanism, where Ca(2+) plays a central regulatory role in the expression of Zic3, an early proneural gene, and in epidermal determination which only occurs when the Ca(2+)-dependent signalling pathways are inactive.
Antoniotti,
Control of endothelial cell proliferation by calcium influx and arachidonic acid metabolism: a pharmacological approach.
2003, Pubmed
Antoniotti,
Control of endothelial cell proliferation by calcium influx and arachidonic acid metabolism: a pharmacological approach.
2003,
Pubmed
Barth,
The uptake of Na-22 during induction in presumptive epidermis cells of the rana pipiens gastrula.
1967,
Pubmed
Batut,
[xMLP is an early response calcium target gene in neural determination in Xenopus laevis].
2003,
Pubmed
,
Xenbase
Batut,
The Ca2+-induced methyltransferase xPRMT1b controls neural fate in amphibian embryo.
2005,
Pubmed
,
Xenbase
Bertrand,
Neural tissue in ascidian embryos is induced by FGF9/16/20, acting via a combination of maternal GATA and Ets transcription factors.
2003,
Pubmed
Carrión,
DREAM is a Ca2+-regulated transcriptional repressor.
1999,
Pubmed
Delaune,
Neural induction in Xenopus requires early FGF signalling in addition to BMP inhibition.
2005,
Pubmed
,
Xenbase
Distasi,
Basic fibroblast growth factor opens calcium-permeable channels in quail mesencephalic neural crest neurons.
1995,
Pubmed
Faure,
Endogenous patterns of TGFbeta superfamily signaling during early Xenopus development.
2000,
Pubmed
,
Xenbase
Gomez-Ospina,
The C terminus of the L-type voltage-gated calcium channel Ca(V)1.2 encodes a transcription factor.
2006,
Pubmed
Grunz,
Neural differentiation of Xenopus laevis ectoderm takes place after disaggregation and delayed reaggregation without inducer.
1989,
Pubmed
,
Xenbase
Keller,
Regional expression, pattern and timing of convergence and extension during gastrulation of Xenopus laevis.
1988,
Pubmed
,
Xenbase
Kuroda,
Neural induction in Xenopus: requirement for ectodermal and endomesodermal signals via Chordin, Noggin, beta-Catenin, and Cerberus.
2004,
Pubmed
,
Xenbase
Launay,
A truncated FGF receptor blocks neural induction by endogenous Xenopus inducers.
1996,
Pubmed
,
Xenbase
Leclerc,
Calcium transients triggered by planar signals induce the expression of ZIC3 gene during neural induction in Xenopus.
2003,
Pubmed
,
Xenbase
Leclerc,
L-type calcium channel activation controls the in vivo transduction of the neuralizing signal in the amphibian embryos.
1997,
Pubmed
Leclerc,
Noggin upregulates Fos expression by a calcium-mediated pathway in amphibian embryos.
1999,
Pubmed
Leclerc,
Imaging patterns of calcium transients during neural induction in Xenopus laevis embryos.
2000,
Pubmed
,
Xenbase
Leclerc,
[Neural determination in Xenopus laevis embryos: control of early neural gene expression by calcium].
2001,
Pubmed
,
Xenbase
Moreau,
Increased internal Ca2+ mediates neural induction in the amphibian embryo.
1994,
Pubmed
Nakata,
Xenopus Zic3, a primary regulator both in neural and neural crest development.
1997,
Pubmed
,
Xenbase
Nishinakamura,
Xenopus FK 506-binding protein homolog induces a secondary axis in frog embryos, which is inhibited by coexisting BMP 4 signaling.
1997,
Pubmed
,
Xenbase
Saint-Jeannet,
Modulation of neural commitment by changes in target cell contacts in Pleurodeles waltl.
1990,
Pubmed
Saint-Jeannet,
Expression of N-CAM precedes neural induction in Pleurodeles waltl (urodele, amphibian).
1989,
Pubmed
,
Xenbase
Saint-Jeannet,
Experimentally provoked neural induction results in an incomplete expression of neuronal traits.
1993,
Pubmed
Saneyoshi,
Molecular cloning and expression profile of Xenopus calcineurin A subunit(1).
2000,
Pubmed
,
Xenbase
Sasai,
Ectodermal patterning in vertebrate embryos.
1997,
Pubmed
,
Xenbase
Stern,
Neural induction: old problem, new findings, yet more questions.
2005,
Pubmed
,
Xenbase
Webb,
Localized calcium transients accompany furrow positioning, propagation, and deepening during the early cleavage period of zebrafish embryos.
1997,
Pubmed
Witta,
XIPOU 2, a noggin-inducible gene, has direct neuralizing activity.
1995,
Pubmed
,
Xenbase