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Nucleic Acids Res
1996 Sep 15;2418:3514-21. doi: 10.1093/nar/24.18.3514.
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Genes encoding isoforms of transcription elongation factor TFIIS in Xenopus and the use of multiple unusual RNA processing signals.
Plant KE
,
Hair A
,
Morgan GT
.
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We have identified cDNAs encoding three related forms of transcription elongation factor TFIIS (S-II) in Xenopus laevis ovary. Comparison of Xenopus and mammalian sequences identifies likely diagnostic amino acids that distinguish classes of vertebrate TFIIS. The diversity of TFIIS polypeptides in Xenopus is due partly to the presence of two diverged genes in this tetraploid genome. We isolated genomic clones containing one of the genes, xTFIIS.oA, and, unlike a previously described vertebrate TFIIS gene, found that it contains introns. Alternative splicing at a CAG/CAG motif containing the 3' splice site of intron 4 produces the third form of xTFIIS, which differs from one of the others simply in lacking Ser109. Intron 6 of xTFIIS.oA contains splice and branch site consensus sequences conforming to those of the minor class of AT-AC introns and this was confirmed for the homeologous xTFIIS.oB gene by genomic PCR. Other unusual but functional variants of RNA processing signals were found in xTFIIS genes at the 5' splice site of intron 8 and the polyadenylation hexanucleotides. Utilization of multiple unusual processing signals may make the generation of mature xTFIIS.o mRNAs inefficient and the possible regulatory consequences of this are discussed.
Aebi,
5' cleavage site in eukaryotic pre-mRNA splicing is determined by the overall 5' splice region, not by the conserved 5' GU.
1987, Pubmed
Aebi,
5' cleavage site in eukaryotic pre-mRNA splicing is determined by the overall 5' splice region, not by the conserved 5' GU.
1987,
Pubmed
Ashfield,
Transcriptional termination between the closely linked human complement genes C2 and factor B: common termination factor for C2 and c-myc?
1991,
Pubmed
Aso,
Elongin (SIII): a multisubunit regulator of elongation by RNA polymerase II.
1995,
Pubmed
Aso,
The RNA polymerase II elongation complex.
1995,
Pubmed
Bengal,
Role of the mammalian transcription factors IIF, IIS, and IIX during elongation by RNA polymerase II.
1991,
Pubmed
Bentley,
Regulation of transcriptional elongation by RNA polymerase II.
1995,
Pubmed
Beyer,
Splice site selection, rate of splicing, and alternative splicing on nascent transcripts.
1988,
Pubmed
Burton,
RAP30/74: a general initiation factor that binds to RNA polymerase II.
1988,
Pubmed
Chafin,
Identification and purification of a yeast protein that affects elongation by RNA polymerase II.
1991,
Pubmed
Chamberlin,
New models for the mechanism of transcription elongation and its regulation.
,
Pubmed
Chen,
Characterization of a HeLa cDNA clone encoding the human SII protein, an elongation factor for RNA polymerase II.
1992,
Pubmed
Cipres-Palacin,
Cleavage of the nascent transcript induced by TFIIS is insufficient to promote read-through of intrinsic blocks to elongation by RNA polymerase II.
1994,
Pubmed
Enriquez-Harris,
A pause site for RNA polymerase II is associated with termination of transcription.
1991,
Pubmed
Flores,
Factors involved in specific transcription by mammalian RNA polymerase II. Factors IIE and IIF independently interact with RNA polymerase II.
1989,
Pubmed
Gall,
The sphere organelle contains small nuclear ribonucleoproteins.
1989,
Pubmed
,
Xenbase
Graf,
Genetics of Xenopus laevis.
1991,
Pubmed
,
Xenbase
Hair,
Premature termination of tubulin gene transcription in Xenopus oocytes is due to promoter-dependent disruption of elongation.
1993,
Pubmed
,
Xenbase
Hall,
Requirement of U12 snRNA for in vivo splicing of a minor class of eukaryotic nuclear pre-mRNA introns.
1996,
Pubmed
Hall,
Conserved sequences in a class of rare eukaryotic nuclear introns with non-consensus splice sites.
1994,
Pubmed
Hirashima,
Molecular cloning and characterization of cDNA for eukaryotic transcription factor S-II.
1988,
Pubmed
Horikoshi,
Structural relationships of the three stimulatory factors of RNA polymerase II from Ehrlich ascites tumor cells.
1985,
Pubmed
Hughes,
Evolution of duplicate genes in a tetraploid animal, Xenopus laevis.
1993,
Pubmed
,
Xenbase
Jackson,
A reappraisal of non-consensus mRNA splice sites.
1991,
Pubmed
Johnson,
Complexes of yeast RNA polymerase II and RNA are substrates for TFIIS-induced RNA cleavage.
1994,
Pubmed
Jones,
Control of RNA initiation and elongation at the HIV-1 promoter.
1994,
Pubmed
Kanai,
Heterogeneity and tissue-specific expression of eukaryotic transcription factor S-II-related protein mRNA.
1991,
Pubmed
Kerppola,
RNA polymerase: regulation of transcript elongation and termination.
1991,
Pubmed
Krumm,
Common mechanisms for the control of eukaryotic transcriptional elongation.
1993,
Pubmed
LeMaire,
Splicing precedes polyadenylation during Drosophila E74A transcription.
1990,
Pubmed
Lear,
Hierarchy for 5' splice site preference determined in vivo.
1990,
Pubmed
Manrow,
GAG triplets as splice acceptors of last resort. An unusual form of alternative splicing in prothymosin alpha pre-mRNA.
1993,
Pubmed
Marshall,
Drosophila RNA polymerase II elongation factor DmS-II has homology to mouse S-II and sequence similarity to yeast PPR2.
1990,
Pubmed
Middleton,
Premature termination of transcription can be induced on an injected alpha-tubulin gene in Xenopus oocytes.
1990,
Pubmed
,
Xenbase
Nakanishi,
Purification, gene cloning, and gene disruption of the transcription elongation factor S-II in Saccharomyces cerevisiae.
1992,
Pubmed
Nei,
Simple methods for estimating the numbers of synonymous and nonsynonymous nucleotide substitutions.
1986,
Pubmed
Oh,
Isolation and characterization of the gene encoding the Drosophila melanogaster transcriptional elongation factor, TFIIS.
1995,
Pubmed
Park,
Characterization of the gene encoding the human transcriptional elongation factor TFIIS.
1994,
Pubmed
Price,
Fractionation of transcription factors for RNA polymerase II from Drosophila Kc cell nuclear extracts.
1987,
Pubmed
Qian,
Novel zinc finger motif in the basal transcriptional machinery: three-dimensional NMR studies of the nucleic acid binding domain of transcriptional elongation factor TFIIS.
1993,
Pubmed
Rabbitts,
Alternative 3' processing of Xenopus alpha-tubulin mRNAs; efficient use of a CAUAAA polyadenylation signal.
1992,
Pubmed
,
Xenbase
Reinberg,
Factors involved in specific transcription by mammalian RNA polymerase II. Transcription factor IIS stimulates elongation of RNA chains.
1987,
Pubmed
Reines,
Transcription elongation factor SII (TFIIS) enables RNA polymerase II to elongate through a block to transcription in a human gene in vitro.
1989,
Pubmed
Sheets,
Point mutations in AAUAAA and the poly (A) addition site: effects on the accuracy and efficiency of cleavage and polyadenylation in vitro.
1990,
Pubmed
Shelness,
Apolipoprotein II messenger RNA. Transcriptional and splicing heterogeneity yields six 5'-untranslated leader sequences.
1984,
Pubmed
Spencer,
Transcription elongation and eukaryotic gene regulation.
1990,
Pubmed
Tarn,
A novel spliceosome containing U11, U12, and U5 snRNPs excises a minor class (AT-AC) intron in vitro.
1996,
Pubmed
Tennyson,
The human dystrophin gene requires 16 hours to be transcribed and is cotranscriptionally spliced.
1995,
Pubmed
Thompson,
CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice.
1994,
Pubmed
Umehara,
Isolation and characterization of a cDNA encoding a new type of human transcription elongation factor S-II.
1995,
Pubmed
Wright,
Regulation of eukaryotic gene expression by transcriptional attenuation.
1993,
Pubmed
Xu,
Cloning and identification of testis-specific transcription elongation factor S-II.
1994,
Pubmed
Yoo,
Cloning, expression and characterization of the human transcription elongation factor, TFIIS.
1991,
Pubmed
