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XB-ART-28838
Histochemistry 1986 Jan 01;853:185-92. doi: 10.1007/bf00494802.
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Cytophotometric evidence of variation in genome size of desmognathine salamanders.

Hally MK , Rasch EM , Mainwaring HR , Bruce RC .


Abstract
The amount of DNA per haploid genome, the C-value, is often directly correlated with nuclear and cell volume, but inversely correlated with cell replication rate. Also, rates of cellular growth sometimes appear to be correlated with organismal developmental rates and life history patterns. Among vertebrates, salamanders exhibit the greatest variation in genome size. In the present study we have examined interspecific and intraspecific variation in blood cell DNA levels in the genus Desmognathus, which shows greater variation in life history traits than any other salamander genus. Specimens of Desmognathus quadramaculatus, D. monticola, D. ochrophaeus and D. wrighti were collected from nature at two localities in the southern Appalachian Mountains. Estimates of genome size in pg of DNA were obtained from blood smears by DNA-Feulgen cytophotometry, using erythrocyte nuclei of Xenopus laevis as an internal reference standard of 6.35 pg DNA per cell. C-values of Desmognathus are the smallest in the order Caudata. Although significant variation in DNA levels was found among the four species, the differences were small, and do not support previously proposed relationships between C-value and life-history variation.

PubMed ID: 3744902
Article link: Histochemistry



References [+] :
Allison, Increased accuracy of absorption cytophotometric DNA values by control of stain intensity. 1981, Pubmed