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Ribosome loading, but not protein synthesis, is required for estrogen stabilization of Xenopus laevis vitellogenin mRNA.
Blume JE
,
Shapiro DJ
.
Abstract
We have examined the effect of protein synthesis and of ribosome loading on the estrogen-mediated stabilization of hepatic Xenopus laevis vitellogenin mRNA. Removal of estradiol-17 beta from the culture medium, which destabilizes vitellogenin mRNA, does not alter the density of ribosomes on polysomal vitellogenin mRNA, or change the proportion of vitellogenin mRNA associated with the endoplasmic reticulum. Cycloheximide, which inhibits elongation, without changing the density of ribosomes on vitellogenin mRNA, does not block estrogen-mediated stabilization. In contrast, 2-(4-methyl-2,6-dinitroanilino)-N-methylpropionamide, (MDMP), which inhibits initiation, greatly reduces the density of ribosomes on vitellogenin mRNA, and completely blocks estrogen-mediated stabilization. Vitellogenin mRNA in MDMP treated cells is degraded at a rate similar to that seen when untreated cells are transferred from medium containing estrogen to estrogen-free medium. This suggests that a ribosome-associated degradative system may not be responsible for vitellogenin mRNA degradation. The failure of estrogen to stabilize vitellogenin mRNA in MDMP-treated cells is not due to the release of vitellogenin mRNA from the endoplasmic reticulum. Vitellogenin mRNA in MDMP-treated cells remains associated with the endoplasmic reticulum in small polysomes containing 3-5 ribosomes. These data demonstrate that maintaining a high density of ribosomes on vitellogenin mRNA, but not continuing protein synthesis, is necessary for estrogen-mediated stabilization of vitellogenin mRNA.
Baker,
Rapid accumulation of vitellogenin messenger RNA during secondary estrogen stimulation of Xenopus laevis.
1978, Pubmed,
Xenbase
Baker,
Rapid accumulation of vitellogenin messenger RNA during secondary estrogen stimulation of Xenopus laevis.
1978,
Pubmed
,
Xenbase
Barton,
Transient administration of estradiol-17 beta establishes an autoregulatory loop permanently inducing estrogen receptor mRNA.
1988,
Pubmed
,
Xenbase
Berridge,
Characterization of polysomes from Xenopus liver synthesizing vitellogenin and translation of vitellogenin and albumin messenger RNA's in vitro.
1976,
Pubmed
,
Xenbase
Brock,
Estrogen stabilizes vitellogenin mRNA against cytoplasmic degradation.
1983,
Pubmed
,
Xenbase
Brock,
Estrogen regulates the absolute rate of transcription of the Xenopus laevis vitellogenin genes.
1983,
Pubmed
,
Xenbase
Cochrane,
Estrogen-dependent modification of ribosomal proteins. Effects of estrogen withdrawal on the distribution of constitutive and hormonally regulated mRNAs.
1984,
Pubmed
Daar,
Premature translation termination mediates triosephosphate isomerase mRNA degradation.
1988,
Pubmed
Gerber-Huber,
Precursor-product relationship between vitellogenin and the yolk proteins as derived from the complete sequence of a Xenopus vitellogenin gene.
1987,
Pubmed
,
Xenbase
Graves,
Translation is required for regulation of histone mRNA degradation.
1987,
Pubmed
Hayward,
Induction of estrogen receptor and reversal of the nuclear/cytoplasmic receptor ratio during vitellogenin synthesis and withdrawal in Xenopus laevis.
1980,
Pubmed
,
Xenbase
Hoekema,
Codon replacement in the PGK1 gene of Saccharomyces cerevisiae: experimental approach to study the role of biased codon usage in gene expression.
1987,
Pubmed
Losson,
Interference of nonsense mutations with eukaryotic messenger RNA stability.
1979,
Pubmed
McKearin,
Estrogen induces transcription of the Xenopus laevis serum retinol-binding protein gene.
1987,
Pubmed
,
Xenbase
Pachter,
Autoregulation of tubulin expression is achieved through specific degradation of polysomal tubulin mRNAs.
1987,
Pubmed
Pandey,
The stem-loop structure at the 3' end of histone mRNA is necessary and sufficient for regulation of histone mRNA stability.
1987,
Pubmed
Ramsey,
A method for visualizing and quantifying the total complement of free and membrane-bound ribosomes in rat liver.
1979,
Pubmed
Ross,
Properties of the exonuclease activity that degrades H4 histone mRNA.
1987,
Pubmed
Ross,
The turnover of messenger RNA.
1989,
Pubmed
Shapiro,
Regulation of messenger RNA stability in eukaryotic cells.
1987,
Pubmed
Weeks,
Specific inhibition of peptide-chain initiation by 2-(4-methyl-2,6-dinitroanilino)-N-methylpropionamide.
1972,
Pubmed
Yen,
Autoregulated instability of beta-tubulin mRNAs by recognition of the nascent amino terminus of beta-tubulin.
1988,
Pubmed
Yen,
Autoregulated changes in stability of polyribosome-bound beta-tubulin mRNAs are specified by the first 13 translated nucleotides.
1988,
Pubmed