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Abstract
Xenopus oocytes were injected with poly(A)+ mRNA obtained from the electric lobes of Torpedo marmorata and Torpedo ocellata, which contain the cell bodies of the neurons that innervate the electric organs. The electric lobe mRNA preparation induces the oocytes to synthesize a catalytically active form of the enzyme choline acetyltransferase (EC 2.3.1.6). Enzymatic activity is found almost exclusively in the cytoplasmic fraction of injected, but not control, oocytes. Evidence is presented that distinguishes between the induced choline acetyltransferase activity and an intrinsic carnitine acetyltransferase activity present in the oocytes. This latter enzyme is associated principally with particulate fractions of the oocyte. The level of acetylcholine, which accumulates in mRNA-injected oocytes, is relatively insensitive to pharmacological manipulations that alter the acetylcholine content of other cells. These results show that Xenopus oocytes may be used advantageously to study functional properties of polypeptides associated with presynaptic elements in the nervous system.
Abe,
Isolation and characterization of presynaptically acting neurotoxins from the venom of Bungarus snakes.
1977, Pubmed
Abe,
Isolation and characterization of presynaptically acting neurotoxins from the venom of Bungarus snakes.
1977,
Pubmed
Adamson,
Changes in the rate of histone synthesis during oocyte maturation and very early development of Xenopus laevis.
1977,
Pubmed
,
Xenbase
Asselbergs,
Post-synthetic fate of the translation products of messenger RNA microinjected into Xenopus oocytes.
1979,
Pubmed
,
Xenbase
Aviv,
Purification of biologically active globin messenger RNA by chromatography on oligothymidylic acid-cellulose.
1972,
Pubmed
Barnard,
Translation of exogenous messenger RNA coding for nicotinic acetylcholine receptors produces functional receptors in Xenopus oocytes.
1982,
Pubmed
,
Xenbase
Bremer,
Carnitine--metabolism and functions.
1983,
Pubmed
Dowdall,
Recent studies on the comparative biochemistry of the cholinergic neuron.
1976,
Pubmed
Fonnum,
A rapid radiochemical method for the determination of choline acetyltransferase.
1975,
Pubmed
Ford,
A method for enucleating oocytes of Xenopus laevis.
1977,
Pubmed
,
Xenbase
Freeman,
Plasma choline: its turnover and exchange with brain choline.
1975,
Pubmed
Gundersen,
Beta-bungarotoxin stimulates the synthesis and accumulation of acetylcholine in rat phrenic nerve diaphragm preparations.
1981,
Pubmed
Gundersen,
Acetylcholinesterase activity of Xenopus laevis oocytes.
1983,
Pubmed
,
Xenbase
Gundersen,
Slowly inactivating potassium channels induced in Xenopus oocytes by messenger ribonucleic acid from Torpedo brain.
1984,
Pubmed
,
Xenbase
Gurdon,
Use of frog eggs and oocytes for the study of messenger RNA and its translation in living cells.
1971,
Pubmed
,
Xenbase
Jenden,
Simultaneous measurement of endogenous and deuterium-labeled tracer variants of choline and acetylcholine in subpicomole quantities by gas chromatography-mass spectrometry.
1973,
Pubmed
Lane,
The fate of genes, messengers, and proteins introduced into Xenopus oocytes.
1983,
Pubmed
,
Xenbase
Miledi,
A calcium-dependent transient outward current in Xenopus laevis oocytes.
1982,
Pubmed
,
Xenbase
Morel,
Determination of ACh concentration in torpedo synaptosomes.
1978,
Pubmed
Ohlsson,
Synthesis and insertion, both in vivo and in vitro, of rat-liver cytochrome P-450 and epoxide hydratase into Xenopus laevis membranes.
1981,
Pubmed
,
Xenbase
Robbins,
Investigation of possible cholinergic mechanisms in fertilization of Xenopus eggs.
1981,
Pubmed
,
Xenbase
Rossier,
Choline acetyltransferase: a review with special reference to its cellular and subcellular localization.
1977,
Pubmed
Rowell,
(2-Benzoylethyl)trimethylammonium chloride: a new, selective and stable inhibitor of human placental choline acetyl transferase.
1978,
Pubmed
Schmid,
The isolation, from electromotor neurone perikarya, of messenger RNAs coding for synaptic proteins.
1982,
Pubmed
,
Xenbase
Soreq,
Biosynthesis and secretion of catalytically active acetylcholinesterase in Xenopus oocytes microinjected with mRNA from rat brain and from Torpedo electric organ.
1982,
Pubmed
,
Xenbase
