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Selective interactions among the multiple connexin proteins expressed in the vertebrate lens: the second extracellular domain is a determinant of compatibility between connexins.
White TW
,
Bruzzone R
,
Wolfram S
,
Paul DL
,
Goodenough DA
.
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Gap junctions are collections of intercellular channels composed of structural proteins called connexins (Cx). We have examined the functional interactions of the three rodent connexins present in the lens, Cx43, Cx46, and Cx50, by expressing them in paired Xenopus oocytes. Homotypic channels containing Cx43, Cx46, or Cx50 all developed high conductance. heterotypic channels composed of Cx46 paired with either Cx43 or Cx50 were also well coupled, whereas Cx50 did not form functional channels with Cx43. We also examined the functional response of homotypic and heterotypic channels to transjunctional voltage and cytoplasmic acidification. We show that all lens connexins exhibited sensitivity to cytoplasmic acidification as well as to voltage, and that voltage-dependent closure of heterotypic channels for a given connexin was dramatically influenced by its partner connexins in the adjacent cell. Based on the observation that Cx43 can discriminate between Cx46 and Cx50, we investigated the molecular determinants that specify compatibility by constructing chimeric connexins from portions of Cx46 and Cx50 and testing them for their ability to form channels with Cx43. When the second extracellular (E2) domain in Cx46 was replaced with the E2 of Cx50, the resulting chimera could no longer form heterotypic channels with Cx43. A reciprocal chimera, where the E2 of Cx46 was inserted into Cx50, acquired the ability to functionally interact with Cx43. Together, these results demonstrate that formation of intercellular channels is a selective process dependent on the identity of the connexins expressed in adjacent cells, and that the second extracellular domain is a determinant of heterotypic compatibility between connexins.
Barrio,
Gap junctions formed by connexins 26 and 32 alone and in combination are differently affected by applied voltage.
1991, Pubmed,
Xenbase
Barrio,
Gap junctions formed by connexins 26 and 32 alone and in combination are differently affected by applied voltage.
1991,
Pubmed
,
Xenbase
Bassnett,
The influence of pH on membrane conductance and intercellular resistance in the rat lens.
1988,
Pubmed
Benedetti,
Development of junctions during differentiation of lens fibers.
1974,
Pubmed
Bennett,
Gap junctions: new tools, new answers, new questions.
1991,
Pubmed
Bennett,
Biophysics of gap junctions.
1992,
Pubmed
Beyer,
Connexin43: a protein from rat heart homologous to a gap junction protein from liver.
1987,
Pubmed
Beyer,
Antisera directed against connexin43 peptides react with a 43-kD protein localized to gap junctions in myocardium and other tissues.
1989,
Pubmed
Beyer,
Connexin family of gap junction proteins.
1990,
Pubmed
Bruzzone,
Connexin40, a component of gap junctions in vascular endothelium, is restricted in its ability to interact with other connexins.
1993,
Pubmed
,
Xenbase
Chanson,
Extent and modulation of junctional communication between pancreatic acinar cells in vivo.
1991,
Pubmed
Dahl,
Expression of functional cell-cell channels from cloned rat liver gap junction complementary DNA.
1987,
Pubmed
,
Xenbase
Dahl,
Cell/cell channel formation involves disulfide exchange.
1991,
Pubmed
Dahl,
Mutational analysis of gap junction formation.
1992,
Pubmed
,
Xenbase
Dermietzel,
Gap junctions in the brain: where, what type, how many and why?
1993,
Pubmed
,
Xenbase
Ebihara,
Cloning and expression of a Xenopus embryonic gap junction protein.
1989,
Pubmed
,
Xenbase
Ebihara,
Properties of a nonjunctional current expressed from a rat connexin46 cDNA in Xenopus oocytes.
1993,
Pubmed
,
Xenbase
Emptage,
Internal acidification modulates membrane and junctional resistance in the isolated lens of the frog Rana pipiens.
1992,
Pubmed
Epstein,
A study of communication specificity between cells in culture.
1977,
Pubmed
Evans,
Gap junction formation during development of the mouse lens.
1993,
Pubmed
Fishman,
Functional analysis of human cardiac gap junction channel mutants.
1991,
Pubmed
Goodenough,
Lens metabolic cooperation: a study of mouse lens transport and permeability visualized with freeze-substitution autoradiography and electron microscopy.
1980,
Pubmed
Goodenough,
Topological distribution of two connexin32 antigenic sites in intact and split rodent hepatocyte gap junctions.
1988,
Pubmed
Haefliger,
Four novel members of the connexin family of gap junction proteins. Molecular cloning, expression, and chromosome mapping.
1992,
Pubmed
Hennemann,
Two gap junction genes, connexin 31.1 and 30.3, are closely linked on mouse chromosome 4 and preferentially expressed in skin.
1992,
Pubmed
,
Xenbase
Hennemann,
Molecular cloning and functional expression of mouse connexin40, a second gap junction gene preferentially expressed in lung.
1992,
Pubmed
,
Xenbase
Hennemann,
Characterization of gap junction genes expressed in F9 embryonic carcinoma cells: molecular cloning of mouse connexin31 and -45 cDNAs.
1992,
Pubmed
Hoh,
Molecular cloning and characterization of a new member of the gap junction gene family, connexin-31.
1991,
Pubmed
,
Xenbase
Jiang,
Molecular cloning and functional characterization of chick lens fiber connexin 45.6.
1994,
Pubmed
,
Xenbase
Kistler,
Identification of a 70,000-D protein in lens membrane junctional domains.
1985,
Pubmed
Krieg,
Functional messenger RNAs are produced by SP6 in vitro transcription of cloned cDNAs.
1984,
Pubmed
,
Xenbase
Kumar,
Molecular biology and genetics of gap junction channels.
1992,
Pubmed
Kuszak,
Lens optical quality is a direct function of lens sutural architecture.
1991,
Pubmed
Liu,
A structural basis for the unequal sensitivity of the major cardiac and liver gap junctions to intracellular acidification: the carboxyl tail length.
1993,
Pubmed
,
Xenbase
Lo,
Gap junctional communication in the post-implantation mouse embryo.
1979,
Pubmed
Lo,
Multiple structural types of gap junctions in mouse lens.
1993,
Pubmed
Mathias,
Cell to cell communication and pH in the frog lens.
1991,
Pubmed
Milks,
Topology of the 32-kd liver gap junction protein determined by site-directed antibody localizations.
1988,
Pubmed
Miller,
Evidence for two physiologically distinct gap junctions expressed by the chick lens epithelial cell.
1986,
Pubmed
Moreno,
Phosphorylation shifts unitary conductance and modifies voltage dependent kinetics of human connexin43 gap junction channels.
1992,
Pubmed
Musil,
Expression of the gap junction protein connexin43 in embryonic chick lens: molecular cloning, ultrastructural localization, and post-translational phosphorylation.
1990,
Pubmed
Nicholson,
Two homologous protein components of hepatic gap junctions.
,
Pubmed
Paul,
Molecular cloning of cDNA for rat liver gap junction protein.
1986,
Pubmed
Paul,
Connexin46, a novel lens gap junction protein, induces voltage-gated currents in nonjunctional plasma membrane of Xenopus oocytes.
1991,
Pubmed
,
Xenbase
Rae,
The electrophysiology of the crystalline lens.
1979,
Pubmed
Rae,
The electrical coupling of epithelium and fibers in the frog lens.
1983,
Pubmed
Revel,
Hexagonal array of subunits in intercellular junctions of the mouse heart and liver.
1967,
Pubmed
Rubin,
A domain substitution procedure and its use to analyze voltage dependence of homotypic gap junctions formed by connexins 26 and 32.
1992,
Pubmed
,
Xenbase
Rup,
Chick connexin-56, a novel lens gap junction protein. Molecular cloning and functional expression.
1993,
Pubmed
Schuetze,
Dye transfer between cells of the embryonic chick lens becomes less sensitive to CO2 treatment with development.
1982,
Pubmed
Spray,
Gap junctional conductance is a simple and sensitive function of intracellular pH.
1981,
Pubmed
Spray,
Sensitivity of gap junctional conductance to H ions in amphibian embryonic cells is independent of voltage sensitivity.
1986,
Pubmed
Spray,
Gating of gap junction channels as revealed in cells stably transfected with wild type and mutant connexin cDNAs.
1992,
Pubmed
Swenson,
Formation of gap junctions by expression of connexins in Xenopus oocyte pairs.
1989,
Pubmed
,
Xenbase
Turin,
Carbon dioxide reversibly abolishes ionic communication between cells of early amphibian embryo.
1977,
Pubmed
,
Xenbase
Warner,
Permeability of gap junctions at the segmental border in insect epidermis.
1982,
Pubmed
Werner,
Formation of hybrid cell-cell channels.
1989,
Pubmed
,
Xenbase
White,
Mouse Cx50, a functional member of the connexin family of gap junction proteins, is the lens fiber protein MP70.
1992,
Pubmed
,
Xenbase
Willecke,
Mouse connexin37: cloning and functional expression of a gap junction gene highly expressed in lung.
1991,
Pubmed
,
Xenbase
Yancey,
The 43-kD polypeptide of heart gap junctions: immunolocalization, topology, and functional domains.
1989,
Pubmed
Zhang,
Sequence and tissue distribution of a second protein of hepatic gap junctions, Cx26, as deduced from its cDNA.
1989,
Pubmed
Zimmer,
Topological analysis of the major protein in isolated intact rat liver gap junctions and gap junction-derived single membrane structures.
1987,
Pubmed