Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
A novel helicase-type protein in the nucleolus: protein NOH61.
Zirwes RF
,
Eilbracht J
,
Kneissel S
,
Schmidt-Zachmann MS
.
???displayArticle.abstract???
We report the identification, cDNA cloning, and molecular characterization of a novel, constitutive nucleolar protein. The cDNA-deduced amino acid sequence of the human protein defines a polypeptide of a calculated mass of 61.5 kDa and an isoelectric point of 9.9. Inspection of the primary sequence disclosed that the protein is a member of the family of "DEAD-box" proteins, representing a subgroup of putative ATP-dependent RNA helicases. ATPase activity of the recombinant protein is evident and stimulated by a variety of polynucleotides tested. Immunolocalization studies revealed that protein NOH61 (nucleolar helicase of 61 kDa) is highly conserved during evolution and shows a strong accumulation in nucleoli. Biochemical experiments have shown that protein NOH61 synthesized in vitro sediments with approximately 11.5 S, i.e., apparently as homo-oligomeric structures. By contrast, sucrose gradient centrifugation analysis of cellular extracts obtained with buffers of elevated ionic strength (600 mM NaCl) revealed that the solubilized native protein sediments with approximately 4 S, suggestive of the monomeric form. Interestingly, protein NOH61 has also been identified as a specific constituent of free nucleoplasmic 65S preribosomal particles but is absent from cytoplasmic ribosomes. Treatment of cultured cells with 1) the transcription inhibitor actinomycin D and 2) RNase A results in a complete dissociation of NOH61 from nucleolar structures. The specific intracellular localization and its striking sequence homology to other known RNA helicases lead to the hypothesis that protein NOH61 might be involved in ribosome synthesis, most likely during the assembly process of the large (60S) ribosomal subunit.
Alber,
Structure of the leucine zipper.
1992, Pubmed
Alber,
Structure of the leucine zipper.
1992,
Pubmed
Cairns,
Identification and cDNA cloning of a Xenopus nucleolar phosphoprotein, xNopp180, that is the homolog of the rat nucleolar protein Nopp140.
1995,
Pubmed
,
Xenbase
Chan,
Nucleophosmin/B23 (NPM) oligomer is a major and stable entity in HeLa cells.
1995,
Pubmed
Chan,
Characterization and cellular localization of nucleophosmin/B23 in HeLa cells treated with selected cytotoxic agents (studies of B23-translocation mechanism).
1992,
Pubmed
Chirgwin,
Isolation of biologically active ribonucleic acid from sources enriched in ribonuclease.
1979,
Pubmed
Cordes,
Cytoplasmic annulate lamellae in cultured cells: composition, distribution, and mitotic behavior.
1996,
Pubmed
Cordes,
Intranuclear filaments containing a nuclear pore complex protein.
1993,
Pubmed
,
Xenbase
Cordes,
Identification of protein p270/Tpr as a constitutive component of the nuclear pore complex-attached intranuclear filaments.
1997,
Pubmed
,
Xenbase
Dingwall,
Nuclear targeting sequences--a consensus?
1991,
Pubmed
Dumont,
Oogenesis in Xenopus laevis (Daudin). I. Stages of oocyte development in laboratory maintained animals.
1972,
Pubmed
,
Xenbase
Eichler,
Processing of eukaryotic ribosomal RNA.
1994,
Pubmed
Evans,
Biochemical fractionation of oocytes.
1991,
Pubmed
,
Xenbase
Flores-Rozas,
Characterization of a new RNA helicase from nuclear extracts of HeLa cells which translocates in the 5' to 3' direction.
1993,
Pubmed
Franke,
Widespread occurrence of intermediate-sized filaments of the vimentin-type in cultured cells from diverse vertebrates.
1979,
Pubmed
,
Xenbase
Franke,
The intermediate-sized filaments in rat kangaroo PtK2 cells. I. Morphology in situ.
1978,
Pubmed
Fuller-Pace,
RNA helicases: modulators of RNA structure.
1994,
Pubmed
Fuller-Pace,
DbpA: a DEAD box protein specifically activated by 23s rRNA.
1993,
Pubmed
Grummt,
Regulation of mammalian ribosomal gene transcription by RNA polymerase I.
1999,
Pubmed
Gururajan,
An3 protein encoded by a localized maternal mRNA in Xenopus laevis is an ATPase with substrate-specific RNA helicase activity.
1997,
Pubmed
,
Xenbase
Gururajan,
An3 mRNA encodes an RNA helicase that colocalizes with nucleoli in Xenopus oocytes in a stage-specific manner.
1994,
Pubmed
,
Xenbase
Heid,
Cell type-specific desmosomal plaque proteins of the plakoglobin family: plakophilin 1 (band 6 protein).
1994,
Pubmed
,
Xenbase
Hügle,
Ribocharin: a nuclear Mr 40,000 protein specific to precursor particles of the large ribosomal subunit.
1985,
Pubmed
,
Xenbase
Iggo,
p68 RNA helicase: identification of a nucleolar form and cloning of related genes containing a conserved intron in yeasts.
1991,
Pubmed
Iost,
Ded1p, a DEAD-box protein required for translation initiation in Saccharomyces cerevisiae, is an RNA helicase.
1999,
Pubmed
Kadaré,
Virus-encoded RNA helicases.
1997,
Pubmed
Kleinschmidt,
Soluble acidic complexes containing histones H3 and H4 in nuclei of Xenopus laevis oocytes.
1982,
Pubmed
,
Xenbase
Kozak,
The scanning model for translation: an update.
1989,
Pubmed
Krämer,
The structure and function of proteins involved in mammalian pre-mRNA splicing.
1996,
Pubmed
Köhler,
AND-1, a natural chimeric DNA-binding protein, combines an HMG-box with regulatory WD-repeats.
1997,
Pubmed
,
Xenbase
Lavoie,
Dbp45A encodes a Drosophila DEAD box protein with similarity to a putative yeast helicase involved in ribosome assembly.
1993,
Pubmed
Liang,
Localization of vasa protein to the Drosophila pole plasm is independent of its RNA-binding and helicase activities.
1994,
Pubmed
Linder,
Birth of the D-E-A-D box.
1989,
Pubmed
Mertens,
Plakophilins 2a and 2b: constitutive proteins of dual location in the karyoplasm and the desmosomal plaque.
1996,
Pubmed
Mélèse,
The nucleolus: an organelle formed by the act of building a ribosome.
1995,
Pubmed
Paule,
Polymerase I transcription, termination, and processing.
1993,
Pubmed
Pederson,
The plurifunctional nucleolus.
1998,
Pubmed
Planta,
Global regulators of ribosome biosynthesis in yeast.
1995,
Pubmed
Qu,
The human DEVH-box protein Ski2w from the HLA is localized in nucleoli and ribosomes.
1998,
Pubmed
Reeder,
rRNA synthesis in the nucleolus.
1990,
Pubmed
Scalenghe,
Large scale isolation of nuclei and nucleoli from vitellogenic oocytes of Xenopus laevis.
1978,
Pubmed
,
Xenbase
Scheer,
The nucleolus.
1994,
Pubmed
Scheer,
Structure and function of the nucleolus.
1999,
Pubmed
Schmid,
D-E-A-D protein family of putative RNA helicases.
1992,
Pubmed
Schmid,
Tissue type-specific expression of intermediate filament proteins in a cultured epithelial cell line from bovine mammary gland.
1983,
Pubmed
Schmidt-Zachmann,
A constitutive nucleolar protein identified as a member of the nucleoplasmin family.
1987,
Pubmed
,
Xenbase
Schmidt-Zachmann,
Identification and localization of a novel nucleolar protein of high molecular weight by a monoclonal antibody.
1984,
Pubmed
,
Xenbase
Schmidt-Zachmann,
Molecular characterization of a novel, widespread nuclear protein that colocalizes with spliceosome components.
1998,
Pubmed
,
Xenbase
Schnölzer,
In situ neutralization in Boc-chemistry solid phase peptide synthesis. Rapid, high yield assembly of difficult sequences.
1992,
Pubmed
Serin,
Two RNA-binding domains determine the RNA-binding specificity of nucleolin.
1997,
Pubmed
Shaw,
The nucleolus.
1995,
Pubmed
Smith,
Sno storm in the nucleolus: new roles for myriad small RNPs.
1997,
Pubmed
Sollner-Webb,
Expression of mouse and frog rRNA genes: transcription and processing.
,
Pubmed
Spector,
Associations between distinct pre-mRNA splicing components and the cell nucleus.
1991,
Pubmed
Thomas,
An octamer of histones in chromatin and free in solution.
1975,
Pubmed
Tollervey,
Small nucleolar RNAs guide ribosomal RNA methylation.
1996,
Pubmed
Tollervey,
Function and synthesis of small nucleolar RNAs.
1997,
Pubmed
Tseng,
Dbp5p, a cytosolic RNA helicase, is required for poly(A)+ RNA export.
1998,
Pubmed
,
Xenbase
Uchida,
The catenin/cadherin adhesion system is localized in synaptic junctions bordering transmitter release zones.
1996,
Pubmed
Valdez,
A nucleolar RNA helicase recognized by autoimmune antibodies from a patient with watermelon stomach disease.
1996,
Pubmed
Valdez,
Cloning and characterization of Gu/RH-II binding protein.
1997,
Pubmed
Venema,
Processing of pre-ribosomal RNA in Saccharomyces cerevisiae.
1995,
Pubmed
Wagner,
The DEAH-box protein PRP22 is an ATPase that mediates ATP-dependent mRNA release from the spliceosome and unwinds RNA duplexes.
1998,
Pubmed
Warner,
The nucleolus and ribosome formation.
1990,
Pubmed
Weinstein,
Guided tours: from precursor snoRNA to functional snoRNP.
1999,
Pubmed
Xu,
An RNA-dependent ATPase associated with U2/U6 snRNAs in pre-mRNA splicing.
1996,
Pubmed
Yung,
Effects of actinomycin D analogs on nucleolar phosphoprotein B23 (37,000 daltons/pI 5.1).
1985,
Pubmed
Zirwes,
Identification of a small, very acidic constitutive nucleolar protein (NO29) as a member of the nucleoplasmin family.
1997,
Pubmed
,
Xenbase
de la Cruz,
Unwinding RNA in Saccharomyces cerevisiae: DEAD-box proteins and related families.
1999,
Pubmed
van Nues,
Processing of eukaryotic pre-rRNA: the role of the transcribed spacers.
1995,
Pubmed
