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EMBO J
1986 Jan 01;51:151-5. doi: 10.1002/j.1460-2075.1986.tb04189.x.
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In vitro transcription by Xenopus oocytes RNA polymerase III requires a DNA topoisomerase II activity.
Glikin GC
,
Blangy D
.
Abstract
Extracts of Xenopus laevis oocytes are able to assemble minichromosomes in vitro when they are supplemented with ATP and Mg2+. We have followed the time course of in vitro DNA supercoiling and transcription of polyoma virus closed circular DNA. The transcriptional activity increased with the assembly time of chromatin in the presence of both ATP and Mg2+, but only residual activity was detected in the absence of either of them. We also found that polyoma DNA as well as 5S RNA gene transcription were carried out mainly by an RNA polymerase III. On the other hand, polyoma RNA and 5S RNA synthesis were inhibited by novobiocin in a way that suggested the requirement of a DNA topoisomerase II or DNA gyrase activity for the initiation of transcription.
Bak,
RNA polymerase III control regions in retrovirus LTR, Alu-type repetitive DNA, and papovavirus.
1984, Pubmed
Bak,
RNA polymerase III control regions in retrovirus LTR, Alu-type repetitive DNA, and papovavirus.
1984,
Pubmed
Birkenmeier,
A nuclear extract of Xenopus laevis oocytes that accurately transcribes 5S RNA genes.
1978,
Pubmed
,
Xenbase
Choder,
A direct analysis of transcribed minichromosomes: all transcribed SV40 minichromosomes have a nuclease-hypersensitive region within a nucleosome-free domain.
1984,
Pubmed
De Robertis,
Coupled transcription-translation of DNA injected into Xenopus oocytes.
1977,
Pubmed
,
Xenbase
Dignam,
Accurate transcription initiation by RNA polymerase II in a soluble extract from isolated mammalian nuclei.
1983,
Pubmed
Gamper,
A topological model for transcription based on unwinding angle analysis of E. coli RNA polymerase binary, initiation and ternary complexes.
1982,
Pubmed
Gargiulo,
Assembly of transcriptionally active chromatin in Xenopus oocytes requires specific DNA binding factors.
1984,
Pubmed
,
Xenbase
Gellert,
DNA topoisomerases.
1981,
Pubmed
Glikin,
Chromatin assembly in Xenopus oocytes: in vitro studies.
1984,
Pubmed
,
Xenbase
Glikin,
Escherichia coli single-strand binding protein stabilizes specific denatured sites in superhelical DNA.
1983,
Pubmed
Gottesfeld,
Assembly of transcriptionally active 5S RNA gene chromatin in vitro.
1982,
Pubmed
,
Xenbase
Gurdon,
Gene transfer in amphibian eggs and oocytes.
1981,
Pubmed
Gurdon,
The use of Xenopus oocytes for the expression of cloned genes.
1983,
Pubmed
,
Xenbase
Hamelin,
Nicking-closing enzyme is associated with SV40 DNA in vivo as a sodium dodecyl sulfate-resistant complex.
1979,
Pubmed
Han,
Novobiocin blocks the Drosophila heat shock response.
1985,
Pubmed
Hirose,
Enhanced transcription of fibroin gene in vitro on covalently closed circular templates.
1985,
Pubmed
Hirt,
Selective extraction of polyoma DNA from infected mouse cell cultures.
1967,
Pubmed
Jat,
Comparison of the polyoma virus early and late promoters by transcription in vitro.
1982,
Pubmed
Kmiec,
The positive transcription factor of the 5S RNA gene induces a 5S DNA-specific gyration in Xenopus oocyte extracts.
1985,
Pubmed
,
Xenbase
Krauss,
Polyomavirus minichromosomes: associated DNA topoisomerase II and DNA ligase activities.
1984,
Pubmed
Ladiges,
The canine major histocompatibility complex. Supertypic specificities defined by the primed lymphocyte test (PLT).
1984,
Pubmed
Luchnik,
Elastic torsional strain in DNA within a fraction of SV40 minichromosomes: relation to transcriptionally active chromatin.
1982,
Pubmed
Luchnik,
DNAaseI-hypersensitive minichromosomes of SV40 possess an elastic torsional strain in DNA.
1985,
Pubmed
Manley,
DNA-dependent transcription of adenovirus genes in a soluble whole-cell extract.
1980,
Pubmed
Miller,
Kinetics of accumulation and processing of simian virus 40 RNA in Xenopus laevis oocytes injected with simian virus 40 DNA.
1982,
Pubmed
,
Xenbase
Ng,
Transcription of cloned Xenopus 5S RNA genes by X. laevis RNA polymerase III in reconstituted systems.
1979,
Pubmed
,
Xenbase
Razvi,
A simple procedure for parallel sequence analysis of both strands of 5'-labeled DNA.
1983,
Pubmed
,
Xenbase
Roeder,
Multiple forms of deoxyribonucleic acid-dependent ribonucleic acid polymerase in Xenopus laevis. Isolation and partial characterization.
1974,
Pubmed
,
Xenbase
Rungger,
DNAs of simian virus 40 and polyoma direct the synthesis of viral tumor antigens and capsid proteins in Xenopus oocytes.
1978,
Pubmed
,
Xenbase
Ryoji,
Chromatin assembly in Xenopus oocytes: in vivo studies.
1984,
Pubmed
,
Xenbase
Ryoji,
Structure of the two distinct types of minichromosomes that are assembled on DNA injected in Xenopus oocytes.
1985,
Pubmed
,
Xenbase
Saragosti,
Absence of nucleosomes in a fraction of SV40 chromatin between the origin of replication and the region coding for the late leader RNA.
1980,
Pubmed
Sergeant,
A transcription enhancer acts in vitro over distances of hundreds of base-pairs on both circular and linear templates but not on chromatin-reconstituted DNA.
1984,
Pubmed
Villeponteau,
Torsional stress promotes the DNAase I sensitivity of active genes.
1984,
Pubmed
Weil,
Faithful transcription of eukaryotic genes by RNA polymerase III in systems reconstituted with purified DNA templates.
1979,
Pubmed
,
Xenbase
Wormington,
Developmental regulation of Xenopus 5S RNA genes.
1983,
Pubmed
,
Xenbase
Yaniv,
Regulation of eukaryotic gene expression by transactivating proteins and cis acting DNA elements.
1984,
Pubmed