XB-ART-26088
Int J Pept Protein Res
1990 Feb 01;352:141-6. doi: 10.1111/j.1399-3011.1990.tb00249.x.
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Multiple peptide synthesis using a single support (MPS3).
Abstract
An automated multiple peptide synthesis method to synthesize, cleave, and purify several peptides simultaneously in a single batch has been developed. The technique is based on the synthesis of multiple peptides on a single solid phase support and is easily adapted to manual or to automated methods. The approach relies on coupling of amino acid mixtures to the resin and it has been found that DCC/HOBt gives the best coupling performance. Fast Atom Bombardment Mass Spectrometry (FAB-MS) was used to rapidly and efficiently identify the peptides in each synthetic mixture which significantly assisted the purification process by HPLC. The method has been successfully applied to the synthesis of magainin 2 and angiotensinogen peptides.
PubMed ID: 2323887
Article link: Int J Pept Protein Res
Species referenced: Xenopus laevis
Genes referenced: magainins