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Recombination of DNAs in Xenopus oocytes based on short homologous overlaps.
Grzesiuk E
,
Carroll D
.
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Linear molecules of pBR322 and closely related plasmid DNAs were injected into Xenopus oocyte nuclei. Such molecules were degraded unless their ends were recombined. Non-homologous ends were joined rarely, if at all, but measurable recombination was supported by homologous sequences of less than 10 base pairs (bp). The efficiency of recombination increased as the length and degree of homology improved, in the range of about 8-20 bp. The homologous sequences had to be very close to the original molecular ends (within about 20 bp); internal homologies, even when they included better matches, were never used. These observations are best accommodated by a model of recombination which envisions exonucleolytic resection to expose homologous sequences, followed by annealing of single-stranded tails, tidying up and sealing of the new joint. Some of the recombined plasmids had novel tetracycline resistance genes; their properties give some insight into the function of the tet gene product.
Ayares,
Sequence homology requirements for intermolecular recombination in mammalian cells.
1986, Pubmed
Ayares,
Sequence homology requirements for intermolecular recombination in mammalian cells.
1986,
Pubmed
Boyer,
A complementation analysis of the restriction and modification of DNA in Escherichia coli.
1969,
Pubmed
Brenner,
Effect of insertions, deletions, and double-strand breaks on homologous recombination in mouse L cells.
1985,
Pubmed
Brutlag,
SEQ: a nucleotide sequence analysis and recombination system.
1982,
Pubmed
Carroll,
Genetic recombination of bacteriophage lambda DNAs in Xenopus oocytes.
1983,
Pubmed
,
Xenbase
Carroll,
Efficient homologous recombination of linear DNA substrates after injection into Xenopus laevis oocytes.
1986,
Pubmed
,
Xenbase
Chakrabarti,
Recombination and deletion of sequences in shuttle vector plasmids in mammalian cells.
1985,
Pubmed
Chen,
Supercoil sequencing: a fast and simple method for sequencing plasmid DNA.
1985,
Pubmed
Doherty,
Genetic recombination of bacterial plasmid DNA. Physical and genetic analysis of the products of plasmid recombination in Escherichia coli.
1983,
Pubmed
Folger,
Patterns of integration of DNA microinjected into cultured mammalian cells: evidence for homologous recombination between injected plasmid DNA molecules.
1982,
Pubmed
Folger,
Analysis of homologous recombination in cultured mammalian cells.
1984,
Pubmed
Hanahan,
Studies on transformation of Escherichia coli with plasmids.
1983,
Pubmed
Kucherlapati,
Homologous recombination between plasmids in mammalian cells can be enhanced by treatment of input DNA.
1984,
Pubmed
Lin,
Model for homologous recombination during transfer of DNA into mouse L cells: role for DNA ends in the recombination process.
1984,
Pubmed
Lin,
Recombination in mouse L cells between DNA introduced into cells and homologous chromosomal sequences.
1985,
Pubmed
Maniatis,
Targeting in mammalian cells.
,
Pubmed
Miller,
High-efficiency ligation and recombination of DNA fragments by vertebrate cells.
1983,
Pubmed
Orr-Weaver,
Yeast transformation: a model system for the study of recombination.
1981,
Pubmed
Peden,
Revised sequence of the tetracycline-resistance gene of pBR322.
1983,
Pubmed
Perucho,
Genetic and physical linkage of exogenous sequences in transformed cells.
1980,
Pubmed
Roth,
Nonhomologous recombination in mammalian cells: role for short sequence homologies in the joining reaction.
1986,
Pubmed
Roth,
Relative rates of homologous and nonhomologous recombination in transfected DNA.
1985,
Pubmed
Roth,
Mechanisms of nonhomologous recombination in mammalian cells.
1985,
Pubmed
Rubnitz,
The minimum amount of homology required for homologous recombination in mammalian cells.
1984,
Pubmed
Rubnitz,
Rapid assay for extrachromosomal homologous recombination in monkey cells.
1985,
Pubmed
Sanger,
DNA sequencing with chain-terminating inhibitors.
1977,
Pubmed
Shapira,
Novel use of synthetic oligonucleotide insertion mutants for the study of homologous recombination in mammalian cells.
1983,
Pubmed
Shen,
Homologous recombination in Escherichia coli: dependence on substrate length and homology.
1986,
Pubmed
Singer,
Determination of the amount of homology required for recombination in bacteriophage T4.
1982,
Pubmed
Small,
Recombination during gene transfer into mouse cells can restore the function of deleted genes.
1983,
Pubmed
Smith,
Homologous recombination between defective neo genes in mouse 3T6 cells.
1984,
Pubmed
Smithies,
Insertion of DNA sequences into the human chromosomal beta-globin locus by homologous recombination.
,
Pubmed
Subramani,
Homologous and nonhomologous recombination in monkey cells.
1983,
Pubmed
Thomas,
Recombination of DNA molecules.
1966,
Pubmed
Thomas,
High frequency targeting of genes to specific sites in the mammalian genome.
1986,
Pubmed
Upcroft,
Analysis of recombination in mammalian cells using SV40 genome segments having homologous overlapping termini.
1980,
Pubmed
Wake,
Simian virus 40 recombinants are produced at high frequency during infection with genetically mixed oligomeric DNA.
1979,
Pubmed
Wake,
Topological requirements for homologous recombination among DNA molecules transfected into mammalian cells.
1985,
Pubmed
Watt,
Homology requirements for recombination in Escherichia coli.
1985,
Pubmed
Wilson,
Somatic cells efficiently join unrelated DNA segments end-to-end.
1982,
Pubmed
Wyllie,
Selective DNA conservation and chromatin assembly after injection of SV40 DNA into Xenopus oocytes.
1978,
Pubmed
,
Xenbase
de Saint Vincent,
Homologous recombination in mammalian cells mediates formation of a functional gene from two overlapping gene fragments.
1983,
Pubmed
