XB-ART-31376Proc Natl Acad Sci U S A 1980 Jul 01;777:4147-51.
Show Gene links Show Anatomy links
Transcription of tRNA genes in vivo: single-stranded compared to double-stranded templates.
The expression of cloned tRNA genes has been studied by injecting single-stranded and double-stranded DNA templates into Xenopus oocyte nuclei. In both forms the genes are faithfully transcribed after injection. Some single-stranded DNA is converted into double-stranded DNA in the oocyte nucleus. This conversion is necessary for the expression of the injected tRNA gene: no tRNA transcription is observed when DNA synthesis is inhibited. We conclude that single-stranded DNA does not serve as a template for faithful transcription of this gene in injected oocytes.
PubMed ID: 7001455
PMC ID: PMC349787
Genes referenced: mt-tr trna
References [+] :