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Proc Natl Acad Sci U S A 1980 Jul 01;777:4147-51.
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Transcription of tRNA genes in vivo: single-stranded compared to double-stranded templates.

Cortese R , Harland R , Melton D .

The expression of cloned tRNA genes has been studied by injecting single-stranded and double-stranded DNA templates into Xenopus oocyte nuclei. In both forms the genes are faithfully transcribed after injection. Some single-stranded DNA is converted into double-stranded DNA in the oocyte nucleus. This conversion is necessary for the expression of the injected tRNA gene: no tRNA transcription is observed when DNA synthesis is inhibited. We conclude that single-stranded DNA does not serve as a template for faithful transcription of this gene in injected oocytes.

PubMed ID: 7001455
PMC ID: PMC349787

Genes referenced: mt-tr trna

References [+] :
Brown, High-fidelity transcription of 5S DNA injected into Xenopus oocytes. 1977, Pubmed, Xenbase