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Microtubule protein synthesis during oogenesis and early embryogenesis in Xenopus laevis.
Pestell RQ
.
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A method is described which permits the preparation of descrete classes of oocytes of different sizes from all stages of oogenesis in Xenopus laevis. This technique is used in the determination of the content of microtubule protein in oocytes during the course of oogenesis. These experiments show that microtubule protein is present in oocytes of all sizes assayed and that the amount is simply related to the volume of the oocyte. In the largest oocytes microtubule protein constitutes 1% of the soluble protein and this amount does not change on maturation and fertilization. These results show that the changes occurring in the oocyte on maturation which allow the cytoplasm to support microtubule polymerization occur as a result of a modification of the pre-existing microtubule protein, not from protein synthesis de novo. These experiments also indicate that the synthesis of microtubule protein either form 'masked' mRNA or from newly synthesized mRNA plays an insignificant role in microtubule protein synthesis at maturation, ovulation and immediately post-fertilization.
BARTH,
Differentiation of cells of the Rana pipiens gastrula in unconditioned medium.
1959, Pubmed
BARTH,
Differentiation of cells of the Rana pipiens gastrula in unconditioned medium.
1959,
Pubmed
BROWN,
ABSENCE OF RIBOSOMAL RNA SYNTHESIS IN THE ANUCLEOLATE MUTANT OF XENOPUS LAEVIS.
1964,
Pubmed
,
Xenbase
Borisy,
The mechanism of action of colchicine. Colchicine binding to sea urchin eggs and the mitotic apparatus.
1967,
Pubmed
Borisy,
The mechanism of action of colchicine. Binding of colchincine-3H to cellular protein.
1967,
Pubmed
Gurdon,
Changes in somatic cell nuclei inserted into growing and maturing amphibian oocytes.
1968,
Pubmed
,
Xenbase
Gurdon,
Use of frog eggs and oocytes for the study of messenger RNA and its translation in living cells.
1971,
Pubmed
,
Xenbase
KANE,
THE MITOTIC APPARATUS. PHYSICAL-CHEMICAL FACTORS CONTROLLING STABILITY.
1965,
Pubmed
LOWRY,
Protein measurement with the Folin phenol reagent.
1951,
Pubmed
Lane,
Rabbit haemoglobin synthesis in frog cells: the translation of reticulocyte 9 s RNA in frog oocytes.
1971,
Pubmed
Mairy,
[Biochemical studies on oogenesis. I. RNA synthesis and accumulation during oogenesis of the South African toad Xenopus laevis].
1971,
Pubmed
,
Xenbase
Moar,
Translational capacity of living frog eggs and oocytes, as judged by messenger RNA injection.
1971,
Pubmed
Raff,
Oogenetic origin of messenger RNA for embryonic synthesis of microtubule proteins.
1972,
Pubmed
Raff,
Synthesis and storage of microtubule proteins by sea urchin embryos.
1971,
Pubmed
Shelanski,
Isolation of a protein subunit from microtubules.
1967,
Pubmed
Stadler,
Colchicine-binding proteins in chromatin and membranes.
1972,
Pubmed
Wallace,
Studies on amphibian yolk. 8. The estrogen-induced hepatic synthesis of a serum lipophosphoprotein and its selective uptake by the ovary and trasformation into yolk platelet proteins in Xenopus laevis.
1969,
Pubmed
,
Xenbase
Wallace,
Studies on amphibian yolk. VII. Serum phosphoprotein synthesis by vitellogenic females and estrogen-treated males of Xenopus laevis.
1968,
Pubmed
,
Xenbase
Wilson,
Properties of colchicine binding protein from chick embryo brain. Interactions with vinca alkaloids and podophyllotoxin.
1970,
Pubmed
Wilson,
The biochemical events of mitosis. II. The in vivo and in vitro binding of colchicine in grasshopper embryos and its possible relation to inhibition of mitosis.
1967,
Pubmed
Wolf,
A molecular approach to fertilization. II. Viability and artificial fertilization of Xenopus laevis gemetes.
1971,
Pubmed
,
Xenbase
Woodland,
Determination of the nucleoside triphosphate contents of eggs and oocytes of Xenopus laevis.
1972,
Pubmed
,
Xenbase
