Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
Calf crystallin synthesis in frog cells: the translation of lens-cell 14S RNA in oocytes.
Berns AJ
,
van Kraaikamp M
,
Bloemendal H
,
Lane CD
.
???displayArticle.abstract???
14S RNA isolated from calf-lens polyribosomes was injected into oocytes of the frog Xenopus laevis. Oocytes injected with 14S RNA and buffer contained a protein resembling the A2 chain of calf alpha-crystallin; oocytes injected with buffer alone contained no crystallin-like material. alphaA2 crystallin polypeptides were identified by various criteria: urea-gel electrophoresis under acidic and basic conditions, gel electrophoresis in sodium dodecyl sulfate, N-terminal analysis, and paper chromatography of methionine-containing tryptic peptides. It is concluded that when it is injected into a living frog oocyte, the 14S RNA from lenstissue is reasonably stable and has the properties of an alphaA2 crystallin messenger. The messenger requires no lens cell-specific components for translation within the oocyte, and the translational machinery of the frog cell will accept messenger RNA from a totally different cell type from another species. The A2 chains of alpha-crystallin extracted from lenstissue possess an acetylated N-terminal methionine residue; the N-terminal methionine of alphaA2 chains derived from frog oocytes injected with 14S RNA was also acetylated.
Berns,
Synthesis of lens protein in vitro V. Isolation of messenger-like RNA from lens by high resolution zonal centrifugation.
1971, Pubmed
Berns,
Synthesis of lens protein in vitro V. Isolation of messenger-like RNA from lens by high resolution zonal centrifugation.
1971,
Pubmed
Berns,
Heterologous in vitro synthesis of lens -crystallin polypeptide.
1972,
Pubmed
Bloemendal,
Polyribosomes from calf-lens epithelium.
1966,
Pubmed
Easley,
Combinations of specific color reactions useful in the peptide mapping technique.
1965,
Pubmed
Heywood,
Specificity of mRNA binding factor in eukaryotes.
1970,
Pubmed
Hoenders,
Release of an N-terminal tetrapeptide from alpha-crystallin.
1968,
Pubmed
Hoenders,
N-terminus of alpha-crystallin.
1968,
Pubmed
Housman,
Initiation of haemoglobin synthesis by methionyl-tRNA.
1970,
Pubmed
Hunter,
The origin and nature of the methionine residue initiating the synthesis of haemoglobin in vivo and in vitro.
1971,
Pubmed
Lane,
Rabbit haemoglobin synthesis in frog cells: the translation of reticulocyte 9 s RNA in frog oocytes.
1971,
Pubmed
Lockard,
Identification of mouse haemoglobin messenger RNA.
1971,
Pubmed
Mathews,
Translation of two messenger RNAs from lens in a cell free system from Krebs II ascites cells.
1972,
Pubmed
Mathews,
Translation of globin messenger RNA in a heterologous cell-free system.
1971,
Pubmed
Prichard,
Translation of exogenous messenger RNA for hemoglobin on reticulocyte and liver ribosomes.
1971,
Pubmed
Raftery,
On the aminoethylation of proteins.
1966,
Pubmed
Schoenmakers,
Isolation of non-identical polypeptide chains of -crystallin.
1969,
Pubmed
Schoenmakers,
The subunit structure of alpha-crystallin. Isolation and characterization of the S-carboxymethylated acidic subunits from adult and embryonic origin.
1969,
Pubmed
Spector,
-Crystallin. The isolation and characterization of distinct macromolecular fractions.
1971,
Pubmed
Stavnezer,
Synthesis of a mouse immunoglobulin light chain in a rabbit reticulocyte cell-free system.
1971,
Pubmed
Weber,
The reliability of molecular weight determinations by dodecyl sulfate-polyacrylamide gel electrophoresis.
1969,
Pubmed
