Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
Functional assay of tRNA molecules transcribed from a purified gene.
Corbo L
,
Ciliberto G
,
Traboni C
,
Santamaria R
,
Cimino F
,
Cortese R
,
Salvatore F
.
Abstract
Purified tRNA genes are expressed when microinjected into the nucleus of X.laevis oocytes. In this paper we describe a method to assay the capacity to be aminoacylated of the tRNA transcribed in the frog oocytes. The method exploits the radiochemical purity of the transcript and relies on the binding of aminoacyl-tRNA but not of uncharged tRNA to purified elongation factor EF-Tu. We also present some preliminary results on several single point mutants of tRNAPro from Caenorhabditis elegans. We show that nucleotide 73 of tRNAPro can be substituted by any other nucleotide without loss of acceptor activity. A double mutant, causing transition from G45G46 to A45A46 has lost acceptor activity. Also inactive is a mutant carrying an insertion of a single base in the anticodon loop.
Benoist,
In vivo sequence requirements of the SV40 early promotor region.
1981, Pubmed
Benoist,
In vivo sequence requirements of the SV40 early promotor region.
1981,
Pubmed
Castagnoli,
Processing of eukaryotic tRNA precursors: secondary structure of the precursor specific sequences affects the rate but not the accuracy of processing reactions.
1982,
Pubmed
Chakraburtty,
Effect of sodium bisulfite modification on the arginine acceptance of E. coli tRNA Arg.
1975,
Pubmed
Chambers,
Inactivation of valine acceptor ativity by a C-U missense change in the anticodon of yeast valine transfer ribonucleic acid.
1973,
Pubmed
Ciampi,
Site-directed mutagenesis of a tRNA gene: base alterations in the coding region affect transcription.
1982,
Pubmed
,
Xenbase
Ciliberto,
Relationship between the two components of the split promoter of eukaryotic tRNA genes.
1982,
Pubmed
,
Xenbase
Ciliberto,
Promoter of a eukaryotic tRNAPro gene is composed of three noncontiguous regions.
1982,
Pubmed
,
Xenbase
Cortese,
Transcription of tRNA genes in vivo: single-stranded compared to double-stranded templates.
1980,
Pubmed
,
Xenbase
Cortese,
Cloning of nematode tRNA genes and their expression in the frog oocyte.
1978,
Pubmed
,
Xenbase
Goddard,
Conversion of exposed cytidine residues to uridine residues in Escherichia coli formylmethionine transfer ribonucleic acid.
1972,
Pubmed
Grummt,
Specific transcription of mouse ribosomal DNA in a cell-free system that mimics control in vivo.
1981,
Pubmed
Mathis,
The SV40 early region TATA box is required for accurate in vitro initiation of transcription.
1981,
Pubmed
McCutchan,
An improved method for the purification of tRNA by chromatography on dihydroxyboryl substituted cellulose.
1975,
Pubmed
Melton,
Order and intracellular location of the events involved in the maturation of a spliced tRNA.
1980,
Pubmed
,
Xenbase
Melton,
Transcription of cloned tRNA genes and the nuclear partitioning of a tRNA precursor.
1979,
Pubmed
,
Xenbase
Mirzabekov,
Investigation of recognition sites in valine tRNA I (Baker's yeast) by dissected molecule method.
1974,
Pubmed
Parlato,
The GTPase activity of elongation factor Tu and the 3'-terminal end of aminoacyl-tRNA.
1981,
Pubmed
Prather,
Nucleotide insertion in the anticodon loop of a glycine transfer RNA causes missense suppression.
1981,
Pubmed
Rich,
Structural organization of complexes of transfer RNAs with aminoacyl transfer RNA synthetases.
1977,
Pubmed
Roe,
Kinetics of homologous and heterologous aminoacylation with yeast phenylalanyl transfer ribonucleic acid synthetase.
1973,
Pubmed
Smith,
Mutant tyrosine transfer RNA that can be charged with glutamine.
1973,
Pubmed
Sprinzl,
Compilation of tRNA sequences.
1982,
Pubmed
Squires,
Normal and mutant glycine transfer RNAs.
1971,
Pubmed
Traboni,
A novel method for site-directed mutagenesis: its application to an eukaryotic tRNAPro gene promoter.
1982,
Pubmed
,
Xenbase
Yaniv,
A single mutational modification of a tryptophan-specific transfer RNA permits aminoacylation by glutamine and translation of the codon UAG.
1974,
Pubmed
