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Chromosoma
2007 Aug 01;1164:349-72. doi: 10.1007/s00412-007-0101-0.
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The three-dimensional structure of in vitro reconstituted Xenopus laevis chromosomes by EM tomography.
König P
,
Braunfeld MB
,
Sedat JW
,
Agard DA
.
Abstract
We have studied the in vitro reconstitution of sperm nuclei and small DNA templates to mitotic chromatin in Xenopus laevis egg extracts by three-dimensional (3D) electron microscopy (EM) tomography. Using specifically developed software, the reconstituted chromatin was interpreted in terms of nucleosomal patterns and the overall chromatin connectivity. The condensed chromatin formed from small DNA templates was characterized by aligned arrays of packed nucleosomal clusters having a typical 10-nm spacing between nucleosomes within the same cluster and a 30-nm spacing between nucleosomes in different clusters. A similar short-range nucleosomal clustering was also observed in condensed chromosomes; however, the clusters were smaller, and they were organized in 30- to 40-nm large domains. An analysis of the overall chromatin connectivity in condensed chromosomes showed that the 30-40-nm domains are themselves organized into a regularly spaced and interconnected 3D chromatin network that extends uniformly throughout the chromosomal volume, providing little indication of a systematic large-scale organization. Based on their topology and high degree of interconnectedness, it is unlikely that 30-40-nm domains arise from the folding of local stretches of nucleosomal fibers. Instead, they appear to be formed by the close apposition of more distant chromatin segments. By combining 3D immunolabeling and EM tomography, we found topoisomerase II to be randomly distributed within this network, while the stable maintenance of chromosomes head domain of condensin was preferentially associated with the 30-40-nm chromatin domains. These observations suggest that 30-40-nm domains are essential for establishing long-range chromatin associations that are central for chromosome condensation.
Adolph,
Assembly of chromatin fibers into metaphase chromosomes analyzed by transmission electron microscopy and scanning electron microscopy.
1986, Pubmed
Adolph,
Assembly of chromatin fibers into metaphase chromosomes analyzed by transmission electron microscopy and scanning electron microscopy.
1986,
Pubmed
Akhmedov,
Mammalian SMC3 C-terminal and coiled-coil protein domains specifically bind palindromic DNA, do not block DNA ends, and prevent DNA bending.
1999,
Pubmed
Akhmedov,
Structural maintenance of chromosomes protein C-terminal domains bind preferentially to DNA with secondary structure.
1998,
Pubmed
Almagro,
The mitotic chromosome is an assembly of rigid elastic axes organized by structural maintenance of chromosomes (SMC) proteins and surrounded by a soft chromatin envelope.
2004,
Pubmed
,
Xenbase
Anderson,
Condensin and cohesin display different arm conformations with characteristic hinge angles.
2002,
Pubmed
,
Xenbase
Ball,
The structural maintenance of chromosomes (SMC) family of proteins in mammals.
2001,
Pubmed
Bazett-Jones,
Efficient supercoiling of DNA by a single condensin complex as revealed by electron spectroscopic imaging.
2002,
Pubmed
,
Xenbase
Bednar,
Nucleosomes, linker DNA, and linker histone form a unique structural motif that directs the higher-order folding and compaction of chromatin.
1998,
Pubmed
Belmont,
A three-dimensional approach to mitotic chromosome structure: evidence for a complex hierarchical organization.
1987,
Pubmed
Belmont,
Large-scale chromatin structural domains within mitotic and interphase chromosomes in vivo and in vitro.
1989,
Pubmed
Bode,
From DNA structure to gene expression: mediators of nuclear compartmentalization and dynamics.
2003,
Pubmed
Butler,
Dinucleosomes show compaction by ionic strength, consistent with bending of linker DNA.
1998,
Pubmed
Chen,
IVE (Image Visualization Environment): a software platform for all three-dimensional microscopy applications.
1996,
Pubmed
Cuvier,
A role of topoisomerase II in linking DNA replication to chromosome condensation.
2003,
Pubmed
,
Xenbase
Dong,
Nucleosome positioning is determined by the (H3-H4)2 tetramer.
1991,
Pubmed
Dorigo,
Nucleosome arrays reveal the two-start organization of the chromatin fiber.
2004,
Pubmed
,
Xenbase
Dubochet,
Nucleosome arcs and helices.
1978,
Pubmed
Earnshaw,
Localization of topoisomerase II in mitotic chromosomes.
1985,
Pubmed
Finch,
Solenoidal model for superstructure in chromatin.
1976,
Pubmed
Finch,
Structure of nucleosome core particles of chromatin.
1977,
Pubmed
Fung,
Toward fully automated high-resolution electron tomography.
1996,
Pubmed
Gasser,
Metaphase chromosome structure. Involvement of topoisomerase II.
1986,
Pubmed
Giannasca,
Transitions between in situ and isolated chromatin.
1993,
Pubmed
Harauz,
Three-dimensional reconstruction of a human metaphase chromosome from electron micrographs.
1987,
Pubmed
Hirano,
Condensins, chromosome condensation protein complexes containing XCAP-C, XCAP-E and a Xenopus homolog of the Drosophila Barren protein.
1997,
Pubmed
,
Xenbase
Hirano,
Topoisomerase II does not play a scaffolding role in the organization of mitotic chromosomes assembled in Xenopus egg extracts.
1993,
Pubmed
,
Xenbase
Hirano,
A heterodimeric coiled-coil protein required for mitotic chromosome condensation in vitro.
1994,
Pubmed
,
Xenbase
Horowitz,
The three-dimensional architecture of chromatin in situ: electron tomography reveals fibers composed of a continuously variable zig-zag nucleosomal ribbon.
1994,
Pubmed
Horowitz,
Automated electron microscope tomography of frozen-hydrated chromatin: the irregular three-dimensional zigzag architecture persists in compact, isolated fibers.
1997,
Pubmed
Houchmandzadeh,
Elasticity measurements show the existence of thin rigid cores inside mitotic chromosomes.
1999,
Pubmed
,
Xenbase
Hudson,
Condensin is required for nonhistone protein assembly and structural integrity of vertebrate mitotic chromosomes.
2003,
Pubmed
Iwano,
Globular and fibrous structure in barley chromosomes revealed by high-resolution scanning electron microscopy.
1997,
Pubmed
Jones,
Improved methods for building protein models in electron density maps and the location of errors in these models.
1991,
Pubmed
Kimura,
Chromosome condensation by a human condensin complex in Xenopus egg extracts.
2001,
Pubmed
,
Xenbase
Kimura,
Phosphorylation and activation of 13S condensin by Cdc2 in vitro.
1998,
Pubmed
,
Xenbase
Kimura,
13S condensin actively reconfigures DNA by introducing global positive writhe: implications for chromosome condensation.
1999,
Pubmed
,
Xenbase
Kimura,
Dual roles of the 11S regulatory subcomplex in condensin functions.
2000,
Pubmed
,
Xenbase
Kimura,
ATP-dependent positive supercoiling of DNA by 13S condensin: a biochemical implication for chromosome condensation.
1997,
Pubmed
,
Xenbase
Kireeva,
Visualization of early chromosome condensation: a hierarchical folding, axial glue model of chromosome structure.
2004,
Pubmed
Koshland,
Mitotic chromosome condensation.
1996,
Pubmed
Krajewski,
Modulation of the higher-order folding of chromatin by deletion of histone H3 and H4 terminal domains.
1996,
Pubmed
König,
Use of surface affinity enrichment and cryo-embedding to prepare in vitro reconstituted mitotic chromosomes for EM tomography.
2005,
Pubmed
,
Xenbase
Legagneux,
Multiple roles of Condensins: a complex story.
2004,
Pubmed
Luger,
Crystal structure of the nucleosome core particle at 2.8 A resolution.
1997,
Pubmed
Löwe,
Crystal structure of the SMC head domain: an ABC ATPase with 900 residues antiparallel coiled-coil inserted.
2001,
Pubmed
Maeshima,
A two-step scaffolding model for mitotic chromosome assembly.
2003,
Pubmed
Maeshima,
Chromosome structure: improved immunolabeling for electron microscopy.
2005,
Pubmed
Maresca,
Histone H1 is essential for mitotic chromosome architecture and segregation in Xenopus laevis egg extracts.
2005,
Pubmed
,
Xenbase
Maruyama,
Stereoscopic scanning electron microscopy of the chromosomes in Vicia faba (broad beans).
1983,
Pubmed
McDowall,
Cryo-electron microscopy of vitrified chromosomes in situ.
1986,
Pubmed
Micheli,
Chromosome length and DNA loop size during early embryonic development of Xenopus laevis.
1993,
Pubmed
,
Xenbase
Mullinger,
Disassembly of the mammalian metaphase chromosome into its subunits: studies with ultraviolet light and repair synthesis inhibitors.
1987,
Pubmed
Murray,
Cell cycle extracts.
1991,
Pubmed
Ohsumi,
Chromosome condensation in Xenopus mitotic extracts without histone H1.
1993,
Pubmed
,
Xenbase
Olins,
Visualization of nucleosomes in thin sections by stereo electron microscopy.
1980,
Pubmed
Ono,
Differential contributions of condensin I and condensin II to mitotic chromosome architecture in vertebrate cells.
2003,
Pubmed
,
Xenbase
Ono,
Spatial and temporal regulation of Condensins I and II in mitotic chromosome assembly in human cells.
2004,
Pubmed
Pettersen,
UCSF Chimera--a visualization system for exploratory research and analysis.
2004,
Pubmed
Philpott,
Nucleoplasmin remodels sperm chromatin in Xenopus egg extracts.
1992,
Pubmed
,
Xenbase
Poirier,
Mitotic chromosomes are chromatin networks without a mechanically contiguous protein scaffold.
2002,
Pubmed
Poljak,
Resolving the role of topoisomerase II in chromatin structure and function.
1995,
Pubmed
Pope,
Proteolysis of mitotic chromosomes induces gradual and anisotropic decondensation correlated with a reduction of elastic modulus and structural sensitivity to rarely cutting restriction enzymes.
2006,
Pubmed
Prigent,
Phosphorylation of serine 10 in histone H3, what for?
2003,
Pubmed
Pyne,
Reorganization of chromatin in Xenopus egg extracts: electron microscopic studies.
2001,
Pubmed
,
Xenbase
Ramakrishnan,
Histone H1 and chromatin higher-order structure.
1997,
Pubmed
Rattner,
Radial loops and helical coils coexist in metaphase chromosomes.
1985,
Pubmed
Robinson,
EM measurements define the dimensions of the "30-nm" chromatin fiber: evidence for a compact, interdigitated structure.
2006,
Pubmed
Sakai,
Condensin but not cohesin SMC heterodimer induces DNA reannealing through protein-protein assembly.
2003,
Pubmed
Schalch,
X-ray structure of a tetranucleosome and its implications for the chromatin fibre.
2005,
Pubmed
,
Xenbase
Stack,
A model for chromosome structure during the mitotic and meiotic cell cycles.
2001,
Pubmed
Sumner,
The distribution of topoisomerase II on mammalian chromosomes.
1996,
Pubmed
Sutani,
DNA renaturation activity of the SMC complex implicated in chromosome condensation.
1997,
Pubmed
Swedlow,
The making of the mitotic chromosome: modern insights into classical questions.
2003,
Pubmed
Swedlow,
Multiple chromosomal populations of topoisomerase II detected in vivo by time-lapse, three-dimensional wide-field microscopy.
1993,
Pubmed
Taniguchi,
High-order structure of metaphase chromosomes: evidence for a multiple coiling model.
1986,
Pubmed
Thoma,
Involvement of histone H1 in the organization of the nucleosome and of the salt-dependent superstructures of chromatin.
1979,
Pubmed
Uhlmann,
A silent revolution in chromosome biology.
2014,
Pubmed
Vassetzky,
Topoisomerase II forms multimers in vitro: effects of metals, beta-glycerophosphate, and phosphorylation of its C-terminal domain.
1994,
Pubmed
Wang,
Condensin binding at distinct and specific chromosomal sites in the Saccharomyces cerevisiae genome.
2005,
Pubmed
Ward,
Deoxyribonucleic acid loop domain tertiary structure in mammalian spermatozoa.
1993,
Pubmed
Wolffe,
Chromatin assembly.
1991,
Pubmed
,
Xenbase
Woodcock,
Role of linker histone in chromatin structure and function: H1 stoichiometry and nucleosome repeat length.
2006,
Pubmed
Yao,
Direct detection of linker DNA bending in defined-length oligomers of chromatin.
1990,
Pubmed
ZOBEL,
Electron stains. I. Chemical studies on the interaction of DNA with uranyl salts.
1961,
Pubmed
Zheng,
Structures and interactions of the core histone tail domains.
2003,
Pubmed
Zhimulev,
Intercalary heterochromatin and genetic silencing.
2003,
Pubmed
el-Alfy,
Subdivision of the mitotic cycle into eleven stages, on the basis of the chromosomal changes observed in mouse duodenal crypt cells stained by the DNA-specific Feulgen reaction.
1994,
Pubmed
el-Alfy,
DNA changes involved in the formation of metaphase chromosomes, as observed in mouse duodenal crypt cells stained by osmium-ammine. I. New structures arise during the S phase and condense at prophase into "chromomeres," which fuse at prometaphase into mitotic chromosomes.
1995,
Pubmed
