Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
A study of the organisation of the ribosomal ribonucleic acid gene cluster of Neurospora crassa by means of restriction endonuclease analysis and cloning in bacteriophage lambda.
Cox RA
,
Peden K
.
Abstract
1. Total Neurospora crassa DNA was restricted with endonucleases and fragments carrying rRNA coding sequences were identified by hybridization with Xenopus laevis ribosomal DNA probes. 2. The repeating unit of the rRNA gene cluster was found to be 8.6 kbp, arranged in a head-to-tail fashion. 3. Digestion with Hind III yielded fragments of 3.4 kbp and 5.2 kbp and both were cloned. 4. Digestion with Eco RI yielded fragments of 2.2 kbp, 3.0 kbp and 3.4 kbp; the 3.0 kbp fragment was cloned. 5. Sequences coding for RNA (S-rRNA)1 of the smaller subribosomal particle were found (at least 90%) in the 2.2 kbp EcoRI subfragment of the 5.2 kbp Hind III fragment. 6. The coding sequences for the major RNA species (L-rRNA) of the larger subribosomal particle were located mainly (at least 95%) in the 3.4 kbp Hind III fragment. 7. For comparison, a Hind III digest of total yeast DNA was cloned and recombinants containing a 6.4 kbp rDNA fragment were isolated.
Bell,
Ribosomal RNA genes of Saccharomyces cerevisiae. I. Physical map of the repeating unit and location of the regions coding for 5 S, 5.8 S, 18 S, and 25 S ribosomal RNAs.
1977, Pubmed
Bell,
Ribosomal RNA genes of Saccharomyces cerevisiae. I. Physical map of the repeating unit and location of the regions coding for 5 S, 5.8 S, 18 S, and 25 S ribosomal RNAs.
1977,
Pubmed
Benton,
Screening lambdagt recombinant clones by hybridization to single plaques in situ.
1977,
Pubmed
Brooks,
Redundant DNA of Neurospora crassa.
1972,
Pubmed
Cameron,
Analysis of chromosomal integration and deletions of yeast plasmids.
1977,
Pubmed
Chattopadhyay,
Ribosomal RNA genes of Neurospora: isolation and characterization.
1972,
Pubmed
Clewell,
Supercoiled circular DNA-protein complex in Escherichia coli: purification and induced conversion to an opern circular DNA form.
1969,
Pubmed
Cox,
Structure and function of prokaryotic and eukaryotic ribosomes.
1977,
Pubmed
Cox,
A study of the influence of magnesium ions on the conformation of ribosomal ribonucleic acid and on the stability of the larger subribosomal particle of rabbit reticulocytes.
1976,
Pubmed
Cox,
A novel recombinant of phage lambda and a conserved 3000-base-pair fragment of Xenopus laevis ribosomal deoxyribonucleic acid produced by restriction with endonucleases Hind III/BamI [proceedings].
1978,
Pubmed
,
Xenbase
Cramer,
Construction and restriction endonuclease mapping of hybrid plasmids containing Saccharomyces cerevisiae ribosomal DNA.
1977,
Pubmed
Denhardt,
A membrane-filter technique for the detection of complementary DNA.
1966,
Pubmed
Engberg,
Free ribosomal DNA molecules from Tetrahymena pyriformis GL are giant palindromes.
1976,
Pubmed
Ferguson,
A new electron microscopic technique for establishing the positions of genes: an analysis of the yeast ribosomal RNA coding region.
1978,
Pubmed
Gerbi,
Fine structure of ribosomal RNA. I. Conservation of homologous regions within ribosomal RNA of eukaryotes.
1976,
Pubmed
Gillespie,
A quantitative assay for DNA-RNA hybrids with DNA immobilized on a membrane.
1965,
Pubmed
Glover,
Characterization of six cloned DNAs from Drosophila melanogaster, including one that contains the genes for rRNA.
1975,
Pubmed
Glover,
A novel arrangement of the 18S and 28S sequences in a repeating unit of Drosophila melanogaster rDNA.
1977,
Pubmed
Hagenbüchle,
Conservation of the primary structure at the 3' end of 18S rRNA from eucaryotic cells.
1978,
Pubmed
Hohn,
Packaging recombinant DNA molecules into bacteriophage particles in vitro.
1977,
Pubmed
Jeffreys,
A physical map of the DNA regions flanking the rabbit beta-globin gene.
1977,
Pubmed
Karrer,
The macronuclear ribosomal DNA of Tetrahymena pyriformis is a palindrome.
1976,
Pubmed
Kramer,
Isolation of bacteriophage lambda containing yeast ribosomal RNA genes: screening by in situ RNA hybridization to plaques.
1976,
Pubmed
Kramer,
Divergent transcription in the yeast ribosomal RNA coding region as shown by hybridization to separated strands and sequence analysis of cloned DNA.
1978,
Pubmed
Laskey,
Enhanced autoradiographic detection of 32P and 125I using intensifying screens and hypersensitized film.
1977,
Pubmed
Maniatis,
Nucleotide sequence of the rightward operator of phage lambda.
1975,
Pubmed
Martin,
Hybrid 80S monomers formed from subunits of ribosomes from protozoa, ungi, plants, and mammals.
1970,
Pubmed
Molgaard,
Organisation of genes for ribosomal RNA in Physarum polycephalum.
1976,
Pubmed
Murray,
Lambdoid phages that simplify the recovery of in vitro recombinants.
1977,
Pubmed
Nath,
Restriction analysis of tandemly repeated yeast ribosomal RNA genes.
1978,
Pubmed
Perkins,
The cytogenetics of Neurospora.
1977,
Pubmed
Philippsen,
Unique arrangement of coding sequences for 5 S, 5.8 S, 18 S and 25 S ribosomal RNA in Saccharomyces cerevisiae as determined by R-loop and hybridization analysis.
1978,
Pubmed
Sinclair,
Retention of common nucleotide sequences in the ribosomal deoxyribonucleic acid of eukaryotes and some of their physical characteristics.
1971,
Pubmed
Southern,
Detection of specific sequences among DNA fragments separated by gel electrophoresis.
1975,
Pubmed
Valenzuela,
Ribosomal RNA genes of Saccharomyces cerevisiae. II. Physical map and nucleotide sequence of the 5 S ribosomal RNA gene and adjacent intergenic regions.
1977,
Pubmed
Vogt,
Structure of ribosomal DNA in Physarum polycephalum.
1976,
Pubmed
Wood,
Hybridization of mitochondrial ribosomal RNA.
1969,
Pubmed
