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EMBO J
2002 Jun 03;2111:2746-56. doi: 10.1093/emboj/21.11.2746.
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Cajal body-specific small nuclear RNAs: a novel class of 2'-O-methylation and pseudouridylation guide RNAs.
Darzacq X
,
Jády BE
,
Verheggen C
,
Kiss AM
,
Bertrand E
,
Kiss T
.
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Cajal (coiled) bodies are conserved subnuclear organelles that are present in the nucleoplasm of both animal and plant cells. Although Cajal bodies were first described nearly 100 years ago, their function has remained largely speculative. Here, we describe a novel class of human small nuclear RNAs that localize specifically to Cajal bodies. The small Cajal body-specific RNAs (scaRNAs) are predicted or have already been demonstrated to function as guide RNAs in site-specific synthesis of 2'-O-ribose-methylated nucleotides and pseudouridines in the RNA polymerase II-transcribed U1, U2, U4 and U5 spliceosomal small nuclear RNAs (snRNAs). Our results provide strong support for the idea that the Cajal body, this mysterious nuclear organelle, provides the cellular locale for post-transcriptional modification of spliceosomal snRNAs.
Andrade,
Human autoantibody to a novel protein of the nuclear coiled body: immunological characterization and cDNA cloning of p80-coilin.
1991, Pubmed
Andrade,
Human autoantibody to a novel protein of the nuclear coiled body: immunological characterization and cDNA cloning of p80-coilin.
1991,
Pubmed
Balakin,
The RNA world of the nucleolus: two major families of small RNAs defined by different box elements with related functions.
1996,
Pubmed
Bohmann,
Molecular analysis of the coiled body.
1995,
Pubmed
Carmo-Fonseca,
Transcription-dependent colocalization of the U1, U2, U4/U6, and U5 snRNPs in coiled bodies.
1992,
Pubmed
Carvalho,
The spinal muscular atrophy disease gene product, SMN: A link between snRNP biogenesis and the Cajal (coiled) body.
1999,
Pubmed
Cavaillé,
Targeted ribose methylation of RNA in vivo directed by tailored antisense RNA guides.
1996,
Pubmed
Gall,
Cajal bodies: the first 100 years.
2000,
Pubmed
Ganot,
Nucleolar factors direct the 2'-O-ribose methylation and pseudouridylation of U6 spliceosomal RNA.
1999,
Pubmed
Ganot,
The family of box ACA small nucleolar RNAs is defined by an evolutionarily conserved secondary structure and ubiquitous sequence elements essential for RNA accumulation.
1997,
Pubmed
Ganot,
Site-specific pseudouridine formation in preribosomal RNA is guided by small nucleolar RNAs.
1997,
Pubmed
Goodall,
Analysis of pre-mRNA processing in transfected plant protoplasts.
1990,
Pubmed
Hüttenhofer,
RNomics: an experimental approach that identifies 201 candidates for novel, small, non-messenger RNAs in mouse.
2001,
Pubmed
Jády,
A small nucleolar guide RNA functions both in 2'-O-ribose methylation and pseudouridylation of the U5 spliceosomal RNA.
2001,
Pubmed
Kiss,
Small nucleolar RNA-guided post-transcriptional modification of cellular RNAs.
2001,
Pubmed
Kiss,
Exonucleolytic processing of small nucleolar RNAs from pre-mRNA introns.
1995,
Pubmed
Kiss-László,
Site-specific ribose methylation of preribosomal RNA: a novel function for small nucleolar RNAs.
1996,
Pubmed
Kiss-László,
Sequence and structural elements of methylation guide snoRNAs essential for site-specific ribose methylation of pre-rRNA.
1998,
Pubmed
Lafontaine,
The box H + ACA snoRNAs carry Cbf5p, the putative rRNA pseudouridine synthase.
1998,
Pubmed
Lamond,
Structure and function in the nucleus.
1998,
Pubmed
Lange,
Conserved boxes C and D are essential nucleolar localization elements of U14 and U8 snoRNAs.
1998,
Pubmed
,
Xenbase
Lange,
Transient nucleolar localization Of U6 small nuclear RNA in Xenopus Laevis oocytes.
2000,
Pubmed
,
Xenbase
Lewis,
Like attracts like: getting RNA processing together in the nucleus.
2000,
Pubmed
Lyon,
Inhibition of protein dephosphorylation results in the accumulation of splicing snRNPs and coiled bodies within the nucleolus.
1997,
Pubmed
Matera,
Nuclear bodies: multifaceted subdomains of the interchromatin space.
1999,
Pubmed
Matera,
Nucleoplasmic organization of small nuclear ribonucleoproteins in cultured human cells.
1993,
Pubmed
Matera,
Of coiled bodies, gems, and salmon.
1998,
Pubmed
Narayanan,
Role of the box C/D motif in localization of small nucleolar RNAs to coiled bodies and nucleoli.
1999,
Pubmed
,
Xenbase
Narayanan,
Nucleolar localization signals of box H/ACA small nucleolar RNAs.
1999,
Pubmed
,
Xenbase
Ni,
Small nucleolar RNAs direct site-specific synthesis of pseudouridine in ribosomal RNA.
1997,
Pubmed
Pan,
U2 snRNA sequences that bind U2-specific proteins are dispensable for the function of U2 snRNP in splicing.
1989,
Pubmed
,
Xenbase
Samarsky,
The snoRNA box C/D motif directs nucleolar targeting and also couples snoRNA synthesis and localization.
1998,
Pubmed
Sleeman,
Nuclear organization of pre-mRNA splicing factors.
1999,
Pubmed
Sleeman,
Newly assembled snRNPs associate with coiled bodies before speckles, suggesting a nuclear snRNP maturation pathway.
1999,
Pubmed
Smith,
Interactions of U2 gene loci and their nuclear transcripts with Cajal (coiled) bodies: evidence for PreU2 within Cajal bodies.
2000,
Pubmed
Smith,
Sno storm in the nucleolus: new roles for myriad small RNPs.
1997,
Pubmed
Spector,
Macromolecular domains within the cell nucleus.
1993,
Pubmed
Ségault,
In vitro reconstitution of mammalian U2 and U5 snRNPs active in splicing: Sm proteins are functionally interchangeable and are essential for the formation of functional U2 and U5 snRNPs.
1995,
Pubmed
Tollervey,
Temperature-sensitive mutations demonstrate roles for yeast fibrillarin in pre-rRNA processing, pre-rRNA methylation, and ribosome assembly.
1993,
Pubmed
Tollervey,
Function and synthesis of small nucleolar RNAs.
1997,
Pubmed
Tyc,
U3, U8 and U13 comprise a new class of mammalian snRNPs localized in the cell nucleolus.
1989,
Pubmed
Tycowski,
Modification of U6 spliceosomal RNA is guided by other small RNAs.
1998,
Pubmed
,
Xenbase
Wang,
Crystal structure of a fibrillarin homologue from Methanococcus jannaschii, a hyperthermophile, at 1.6 A resolution.
2000,
Pubmed
Weinstein,
Guided tours: from precursor snoRNA to functional snoRNP.
1999,
Pubmed
Will,
Spliceosomal UsnRNP biogenesis, structure and function.
2001,
Pubmed
Yu,
Modifications of U2 snRNA are required for snRNP assembly and pre-mRNA splicing.
1998,
Pubmed
,
Xenbase
Yu,
Internal modification of U2 small nuclear (sn)RNA occurs in nucleoli of Xenopus oocytes.
2001,
Pubmed
,
Xenbase
Zebarjadian,
Point mutations in yeast CBF5 can abolish in vivo pseudouridylation of rRNA.
1999,
Pubmed
