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Usage of the three termination codons in a single eukaryotic cell, the Xenopus laevis oocyte.
Bienz M
,
Kubli E
,
Kohli J
,
deHenau S
,
Huez G
,
Marbaix G
,
Grosjean H
.
Abstract
Oocytes from Xenopus laevis were injected with purified amber (UAG), ochre (UAA), and opal (UGA) suppressor tRNAs from yeasts. The radioactively labeled proteins translated from the endogenous mRNAs were then separated on two-dimensional gels. All three termination codons are used in a single cell, the Xenopus laevis oocyte. But a surprisingly low number of readthrough polypeptides were observed from the 600 mRNAs studied in comparison to uninjected oocytes. The experimental data are compared with the conclusions obtained from the compilation of all available termination sequences on eukaryotic and prokaryotic mRNAs. This comparison indicates that the apparent resistance of natural termination codons against readthrough, as observed by the microinjection experiments, cannot be explained by tandem or very close second stop codons. Instead it suggests that specific context sequences around the termination codons may play a role in the efficiency of translation termination.
Atkins,
Normal tRNAs promote ribosomal frameshifting.
1979, Pubmed
Atkins,
Normal tRNAs promote ribosomal frameshifting.
1979,
Pubmed
Atkins,
Low activity of -galactosidase in frameshift mutants of Escherichia coli.
1972,
Pubmed
Barrell,
Different pattern of codon recognition by mammalian mitochondrial tRNAs.
1980,
Pubmed
Bienz,
Nonsense suppression in eukaryotes: the use of the Xenopus oocyte as an in vivo assay system.
1980,
Pubmed
,
Xenbase
Bonner,
A film detection method for tritium-labelled proteins and nucleic acids in polyacrylamide gels.
1974,
Pubmed
Bossi,
The influence of codon context on genetic code translation.
1980,
Pubmed
Brenner,
Genetic code: the 'nonsense' triplets for chain termination and their suppression.
1965,
Pubmed
Clarkson,
Reiterated transfer RNA genes of Xenopus laevis.
1973,
Pubmed
,
Xenbase
Cremer,
In vitro suppression of UAG and UGA mutants in the thymidine kinase gene of herpes simplex virus.
1979,
Pubmed
De Ronde,
In vitro suppression of UGA codons in a mitochondrial mRNA.
1980,
Pubmed
Engelberg-Kulka,
UGA suppression by normal tRNA Trp in Escherichia coli: codon context effects.
1981,
Pubmed
Engelberg-Kulka,
The requirement of nonsense suppression for the development of several phages.
1979,
Pubmed
Fox,
Five TGA "stop" codons occur within the translated sequence of the yeast mitochondrial gene for cytochrome c oxidase subunit II.
1979,
Pubmed
Fox,
Leaky +1 and -1 frameshift mutations at the same site in a yeast mitochondrial gene.
1980,
Pubmed
Fradin,
Mapping of yeast tRNAs by two-dimensional electrophoresis on polyacrylamide gels.
1975,
Pubmed
Geller,
A UGA termination suppression tRNATrp active in rabbit reticulocytes.
1980,
Pubmed
Gurdon,
Changes in somatic cell nuclei inserted into growing and maturing amphibian oocytes.
1968,
Pubmed
,
Xenbase
Gurdon,
Injected nuclei in frog oocytes provide a living cell system for the study of transcriptional control.
1976,
Pubmed
,
Xenbase
Hawthorne,
Suppressors in yeast.
1974,
Pubmed
Iserentant,
Studies on the bacteriophage MS2. XLI. Nature of the azure mutation.
1980,
Pubmed
Kaplan,
In vivo translation of amber and ochre codons in Escherichia coli.
1973,
Pubmed
Kohli,
Characterization of a UGA-suppressing serine tRNA from Schizosaccharomyces pombe with the help of a new in vitro assay system for eukaryotic suppressor tRNAs.
1979,
Pubmed
Lu,
The nature of the polypeptide chain termination signal.
1971,
Pubmed
Maxam,
A new method for sequencing DNA.
1977,
Pubmed
O'Farrell,
High resolution two-dimensional electrophoresis of proteins.
1975,
Pubmed
Pelham,
Leaky UAG termination codon in tobacco mosaic virus RNA.
1978,
Pubmed
Philipson,
Translation of MuLV and MSV RNAs in nuclease-treated reticulocyte extracts: enhancement of the gag-pol polypeptide with yeast suppressor tRNA.
1978,
Pubmed
Piper,
Nonsense suppressors of Saccharomyces cerevisiae can be generated by mutation of the tyrosine tRNA anticodon.
1976,
Pubmed
Rafalski,
The nucleotide sequence of a UGA suppressor serine tRNA from Schizosaccharomyces pombe.
1979,
Pubmed
Salser,
The influence of the reading context upon the suppression of nonsense codons. 3.
1969,
Pubmed
Weiner,
A single UGA codon functions as a natural termination signal in the coliphage q beta coat protein cistron.
1973,
Pubmed
Williams,
The nucleotide sequence of the major beta-globin mRNA from Xenopus laevis.
1980,
Pubmed
,
Xenbase
Yahata,
Adjacent effect on suppression efficiency. II. Study on ochre and amber mutants of T4 phage lysozyme.
1970,
Pubmed
Yates,
Effects of ribosomal mutations on the read-through of a chain termination signal: studies on the synthesis of bacteriophage lambda O gene protein in vitro.
1977,
Pubmed