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J Mol Evol
1984 Jan 01;203-4:202-14. doi: 10.1007/bf02104727.
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Evolution of the human sarcomeric-actin genes: evidence for units of selection within the 3' untranslated regions of the mRNAs.
Gunning P
,
Mohun T
,
Ng SY
,
Ponte P
,
Kedes L
.
Abstract
The complete 3' untranslated region (3'UTR) sequence of the human skeletal-actin gene has been compared with the corresponding regions of the rat and chicken skeletal-actin genes. This comparison reveals that the skeletal-actin 3'UTR is composed of conserved and nonconserved segments. By using genomic Southern transfer blots and thermal stability (Tm) measurements, we found that the cardiac-actin gene 3'UTR also consists of conserved and nonconserved segments. Comparison of human and Xenopus laevis cardiac-actin mRNA sequences confirms the presence of a region of high similarity in the 3'UTR. We conclude that subsegments of the 3'UTRs of both skeletal- and cardiac-actin genes of birds and mammals are under considerable selective pressure. This suggests that these conserved sequences may have functional roles in actin-gene expression or regulation, and that these roles might be different for each actin isoform.
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