XB-ART-28077
EMBO J
1987 Jul 01;67:2145-55. doi: 10.1002/j.1460-2075.1987.tb02482.x.
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The yeast homologue of U3 snRNA.
Hughes JM
,
Konings DA
,
Cesareni G
.
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snR17, one of the most abundant capped small nuclear RNAs of Saccharomyces cerevisiae, is equivalent to U3 snRNA of other eukaryotes. It is 328 nucleotides in length, 1.5 times as long as other U3 RNAs, but shares significant homology both in nucleotide sequence and in predicted secondary structure. Human scleroderma antiserum specific to nucleolar U3 RNP can enrich snR17 from sonicated yeast nuclear extracts. Unlike other yeast snRNAs which are encoded by single copy genes, snR17 is encoded by two genetically unlinked genes: SNR17A and SNR17B. The RNA snR17A is more abundant than snR17B. Deleting one or other of the genes has no obvious phenotypic effect, except that the steady-state level of snR17B is increased in snr17a- strains. Haploid strains with both genes deleted are inviable, therefore yeast U3 is essential.
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References [+] :
Ares,
U2 RNA from yeast is unexpectedly large and contains homology to vertebrate U4, U5, and U6 small nuclear RNAs.
1986, Pubmed
Ares, U2 RNA from yeast is unexpectedly large and contains homology to vertebrate U4, U5, and U6 small nuclear RNAs. 1986, Pubmed
Bachellerie, Recognition signals for mouse pre-rRNA processing. A potential role for U3 nucleolar RNA. 1983, Pubmed
Baldari, Plasmids pEMBLY: new single-stranded shuttle vectors for the recovery and analysis of yeast DNA sequences. 1985, Pubmed
Bernstein, Pseudogenes for human small nuclear RNA U3 appear to arise by integration of self-primed reverse transcripts of the RNA into new chromosomal sites. 1983, Pubmed
Black, Pre-mRNA splicing in vitro requires intact U4/U6 small nuclear ribonucleoprotein. 1986, Pubmed
Black, U2 as well as U1 small nuclear ribonucleoproteins are involved in premessenger RNA splicing. 1985, Pubmed
Branlant, U2 RNA shares a structural domain with U1, U4, and U5 RNAs. 1982, Pubmed
Bringmann, Purification of snRNPs U1, U2, U4, U5 and U6 with 2,2,7-trimethylguanosine-specific antibody and definition of their constituent proteins reacting with anti-Sm and anti-(U1)RNP antisera. 1983, Pubmed
Broach, Transformation in yeast: development of a hybrid cloning vector and isolation of the CAN1 gene. 1979, Pubmed
Brody, The "spliceosome": yeast pre-messenger RNA associates with a 40S complex in a splicing-dependent reaction. 1985, Pubmed
Busch, SnRNAs, SnRNPs, and RNA processing. 1982, Pubmed
Calvet, Base-pairing interactions between small nuclear RNAs and nuclear RNA precursors as revealed by psoralen cross-linking in vivo. 1981, Pubmed
Cech, Secondary structure of the Tetrahymena ribosomal RNA intervening sequence: structural homology with fungal mitochondrial intervening sequences. 1983, Pubmed
Chabot, The 3' splice site of pre-messenger RNA is recognized by a small nuclear ribonucleoprotein. 1985, Pubmed
Church, Genomic sequencing. 1984, Pubmed
Ciliberto, Formation of the 3' end on U snRNAs requires at least three sequence elements. 1986, Pubmed , Xenbase
Dente, pEMBL: a new family of single stranded plasmids. 1983, Pubmed
England, 3'-terminal labelling of RNA with T4 RNA ligase. 1978, Pubmed
Epstein, Multiple states of U3 RNA in Novikoff hepatoma nucleoli. 1984, Pubmed
Feinberg, A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity. 1983, Pubmed
Freier, Improved free-energy parameters for predictions of RNA duplex stability. 1986, Pubmed
Frischauf, Lambda replacement vectors carrying polylinker sequences. 1983, Pubmed
Frischauf, A subcloning strategy for DNA sequence analysis. 1980, Pubmed
Galli, Biochemical complementation with RNA in the Xenopus oocyte: a small RNA is required for the generation of 3' histone mRNA termini. 1983, Pubmed , Xenbase
Goodman, Cloning of hormone genes from a mixture of cDNA molecules. 1979, Pubmed
Guarente, Heme regulates transcription of the CYC1 gene of S. cerevisiae via an upstream activation site. 1983, Pubmed
Haber, Methods in sporulation and germination of yeasts. 1975, Pubmed
Hashimoto, A small nuclear ribonucleoprotein associates with the AAUAAA polyadenylation signal in vitro. 1986, Pubmed
Hernandez, Formation of the 3' end of U1 snRNA is directed by a conserved sequence located downstream of the coding region. 1985, Pubmed
Hogeweg, The alignment of sets of sequences and the construction of phyletic trees: an integrated method. 1984, Pubmed
Jacob, A family of small nucleoplasmic RNAs with common structural features. 1984, Pubmed , Xenbase
Kiss, Plant small nuclear RNAs. Nucleolar U3 snRNA is present in plants: partial characterization. 1985, Pubmed
Krainer, Multiple factors including the small nuclear ribonucleoproteins U1 and U2 are necessary for pre-mRNA splicing in vitro. 1985, Pubmed
Krämer, The 5' terminus of the RNA moiety of U1 small nuclear ribonucleoprotein particles is required for the splicing of messenger RNA precursors. 1984, Pubmed
Lerner, Are snRNPs involved in splicing? 1980, Pubmed
Lerner, Antibodies to small nuclear RNAs complexed with proteins are produced by patients with systemic lupus erythematosus. 1979, Pubmed
Lin, Yeast mRNA splicing in vitro. 1985, Pubmed
Lischwe, Purification and partial characterization of a nucleolar scleroderma antigen (Mr = 34,000; pI, 8.5) rich in NG,NG-dimethylarginine. 1985, Pubmed
Luhrmann, Isolation and characterization of rabbit anti-m3 2,2,7G antibodies. 1982, Pubmed
Maniatis, The role of small nuclear ribonucleoprotein particles in pre-mRNA splicing. , Pubmed
Mattaj, Monospecific antibodies reveal details of U2 snRNP structure and interaction between U1 and U2 snRNPs. 1986, Pubmed , Xenbase
Mattaj, Cap trimethylation of U snRNA is cytoplasmic and dependent on U snRNP protein binding. 1986, Pubmed , Xenbase
Mattaj, Nuclear segregation of U2 snRNA requires binding of specific snRNP proteins. 1985, Pubmed , Xenbase
Matter, Molecular characterization of ribonucleoprotein antigens bound by antinuclear antibodies. A diagnostic evaluation. 1982, Pubmed
Mount, The U1 small nuclear RNA-protein complex selectively binds a 5' splice site in vitro. 1983, Pubmed
Padgett, Splicing of messenger RNA precursors is inhibited by antisera to small nuclear ribonucleoprotein. 1983, Pubmed
Parker, A point mutation in the conserved hexanucleotide at a yeast 5' splice junction uncouples recognition, cleavage, and ligation. 1985, Pubmed
Pikielny, Specific small nuclear RNAs are associated with yeast spliceosomes. 1986, Pubmed
Pikielny, mRNA splicing efficiency in yeast and the contribution of nonconserved sequences. 1985, Pubmed
Prestayko, Low molecular weight RNA associated with 28 s nucleolar RNA. 1970, Pubmed
Reddy, Small nuclear RNAs and RNA processing. 1983, Pubmed
Reddy, Primary and secondary structure of U8 small nuclear RNA. 1985, Pubmed
Riedel, Small nuclear RNAs from Saccharomyces cerevisiae: unexpected diversity in abundance, size, and molecular complexity. 1986, Pubmed
Riedel, A subset of yeast snRNA's contains functional binding sites for the highly conserved Sm antigen. 1987, Pubmed , Xenbase
Rothstein, One-step gene disruption in yeast. 1983, Pubmed
Rubin, Preparation of RNA and ribosomes from yeast. 1975, Pubmed
Sanger, DNA sequencing with chain-terminating inhibitors. 1977, Pubmed
Steitz, Structure and function of small ribonucleoproteins from eukaryotic cells. 1982, Pubmed
Stroke, Genes and pseudogenes for rat U3A and U3B small nuclear RNA. 1985, Pubmed , Xenbase
Strub, The cDNA sequences of the sea urchin U7 small nuclear RNA suggest specific contacts between histone mRNA precursor and U7 RNA during RNA processing. 1984, Pubmed , Xenbase
Suh, Isolation and characterization of a human U3 small nucleolar RNA gene. 1986, Pubmed , Xenbase
Takeishi, Isolation and characterization of small nuclear RNAs from Dictyostelium discoideum. 1981, Pubmed
Tollervey, Deletion of a yeast small nuclear RNA gene impairs growth. 1985, Pubmed
Tollervey, Fungal small nuclear ribonucleoproteins share properties with plant and vertebrate U-snRNPs. 1987, Pubmed , Xenbase
Tollervey, A U4-like small nuclear RNA is dispensable in yeast. 1983, Pubmed
Weinberg, Small molecular weight monodisperse nuclear RNA. 1968, Pubmed
Williams, A dynamic programming algorithm for finding alternative RNA secondary structures. 1986, Pubmed
Wise, Yeast contains small nuclear RNAs encoded by single copy genes. 1983, Pubmed
Wise, Dictyostelium small nuclear RNA D2 is homologous to rat nucleolar RNA U3 and is encoded by a dispersed multigene family. 1980, Pubmed
Yang, A small nuclear ribonucleoprotein is required for splicing of adenoviral early RNA sequences. 1981, Pubmed
Yanisch-Perron, Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mp18 and pUC19 vectors. 1985, Pubmed
Zhuang, A compensatory base change in U1 snRNA suppresses a 5' splice site mutation. 1986, Pubmed
Zieve, Small RNA species of the HeLa cell: metabolism and subcellular localization. 1976, Pubmed
Zoller, Oligonucleotide-directed mutagenesis: a simple method using two oligonucleotide primers and a single-stranded DNA template. 1984, Pubmed