Click here to close Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly. We suggest using a current version of Chrome, FireFox, or Safari.
XB-ART-30027
Proc Natl Acad Sci U S A 1983 Nov 01;8021:6436-40.
Show Gene links Show Anatomy links

tRNA transport from the nucleus in a eukaryotic cell: carrier-mediated translocation process.

Zasloff M .


???displayArticle.abstract???
The mechanism by which a tRNA molecule is delivered from the nucleus of a cell to the cytoplasm has been studied in the Xenopus laevis oocyte utilizing nuclear microinjection and manual microdissection techniques. tRNA nuclear transport in this cell resembles a carrier-mediated translocation process rather than diffusion through a simple pore or channel. tRNA transport is saturable by tRNA, with a maximal rate measured to be about 190 X 10(7) molecules per min per nucleus (21 degrees C) in the mature oocyte. Competitive inhibition between two different tRNA species can be demonstrated, suggesting that many tRNA species share a common carrier system. tRNA nuclear transport is sharply dependent on temperature, with an optimal rate observed at 31 degrees C. A single G-to-U substitution at position 57 in the vertebrate tRNAMeti molecule reduces the transport rate of this tRNA by a factor of about 20, implicating this highly conserved region of the tRNA molecule (loop IV) as critical for recognition by the transport mechanism. On morphologic grounds I propose that ribosome-like components surrounding the nuclear pore may function as the tRNA translocation "motor." The tRNA nuclear transport mechanism represents a distinctly eukaryotic process and a site of potential control over cell growth and proliferation.

???displayArticle.pubmedLink??? 6579529
???displayArticle.pmcLink??? PMC390128
???displayArticle.link??? Proc Natl Acad Sci U S A


Genes referenced: mt-tr trna

References [+] :
Bravo, Conditions affecting protein synthesis in amphibian oocytes. 1976, Pubmed, Xenbase