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Rodent gamma-crystallin promoters were recognized as lens-specific promoters in micro-injected Xenopus laevis tadpoles and targeted the expression of the chloramphenicol acetyl transferase (CAT) reporter gene to the tadpolelens. The onset of expression coincided with lens cell formation. The level of expression continued to increase up to 9 days of development (stage 47), stayed at that level till at least day 13 and dropped by only 57% at day 21. In contrast, the level of expression of a non-tissue-specific promoter, the SV40 early promoter, decreased rapidly in the eye during development and was only detectable up to stage 44 (day 5). The stability of the CAT activity in the lens was assessed by delivering a pulse of activity from a heat shock promoter-CAT fusion gene. The half-life of the CAT activity in the eye was the same as that in the tail. The increase in CAT activity in the lens thus depends upon continued activity of the injected gamma-crystallin promoters. Our data demonstrate that mammalian promoters can be used to target gene expression to specific tissues during Xenopus laevis development.
Andres,
Persistence, methylation and expression of vitellogenin gene derivatives after injection into fertilized eggs of Xenopus laevis.
1984, Pubmed,
Xenbase
Andres,
Persistence, methylation and expression of vitellogenin gene derivatives after injection into fertilized eggs of Xenopus laevis.
1984,
Pubmed
,
Xenbase
Delaye,
Short-range order of crystallin proteins accounts for eye lens transparency.
,
Pubmed
Etkin,
Replication, integration and expression of exogenous DNA injected into fertilized eggs of Xenopus laevis.
1984,
Pubmed
,
Xenbase
Etkin,
Distribution, expression and germ line transmission of exogenous DNA sequences following microinjection into Xenopus laevis eggs.
1987,
Pubmed
,
Xenbase
Giebelhaus,
Antisense RNA inhibits expression of membrane skeleton protein 4.1 during embryonic development of Xenopus.
1988,
Pubmed
,
Xenbase
Gurdon,
Gene transfer in amphibian eggs and oocytes.
1981,
Pubmed
Hofmann,
Plasmid and bacteriophage lambda-DNA show differential replication characteristics following injection into fertilized eggs of Xenopus laevis: dependence on period and site of injection.
1990,
Pubmed
,
Xenbase
Hopwood,
Activation of muscle genes without myogenesis by ectopic expression of MyoD in frog embryo cells.
1990,
Pubmed
,
Xenbase
Krone,
Expression of microinjected hsp 70/CAT and hsp 30/CAT chimeric genes in developing Xenopus laevis embryos.
1989,
Pubmed
,
Xenbase
Landel,
Reverse genetics using transgenic mice.
1990,
Pubmed
Lok,
Lens-specific promoter activity of a mouse gamma-crystallin gene.
1985,
Pubmed
McMahon,
Ectopic expression of the proto-oncogene int-1 in Xenopus embryos leads to duplication of the embryonic axis.
1989,
Pubmed
,
Xenbase
Meakin,
A rabbit lens epithelial cell line supports expression of an exogenous crystallin gene characteristic of lens fiber cell differentiation.
1989,
Pubmed
Palmiter,
Germ-line transformation of mice.
1986,
Pubmed
Peek,
Tissue- and species-specific promoter elements of rat gamma-crystallin genes.
1990,
Pubmed
Rusconi,
Transformation of frog embryos with a rabbit beta-globin gene.
1981,
Pubmed
,
Xenbase
Török,
Nucleotide sequences of heat shock activated genes in Drosophila melanogaster. I. Sequences in the regions of the 5' and 3' ends of the hsp 70 gene in the hybrid plasmid 56H8.
1980,
Pubmed
Van Leen,
Developmental expression of crystallin genes: in situ hybridization reveals a differential localization of specific mRNAs.
1987,
Pubmed
Wilson,
Tissue-specific expression of actin genes injected into Xenopus embryos.
1986,
Pubmed
,
Xenbase
Wistow,
Lens crystallins: the evolution and expression of proteins for a highly specialized tissue.
1988,
Pubmed
Yang-Yen,
Transcriptional interference between c-Jun and the glucocorticoid receptor: mutual inhibition of DNA binding due to direct protein-protein interaction.
1990,
Pubmed
