Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
Anal Chem
2016 Jan 05;881:877-82. doi: 10.1021/acs.analchem.5b03496.
Show Gene links
Show Anatomy links
Nearly 1000 Protein Identifications from 50 ng of Xenopus laevis Zygote Homogenate Using Online Sample Preparation on a Strong Cation Exchange Monolith Based Microreactor Coupled with Capillary Zone Electrophoresis.
Zhang Z
,
Sun L
,
Zhu G
,
Cox OF
,
Huber PW
,
Dovichi NJ
.
???displayArticle.abstract???
A sulfonate-silica hybrid strong cation exchange monolith microreactor was synthesized and coupled to a linear polyacrylamide coated capillary for online sample preparation and capillary zone electrophoresis-tandem mass spectrometry (CZE-MS/MS) bottom-up proteomic analysis. The protein sample was loaded onto the microreactor in an acidic buffer. After online reduction, alkylation, and digestion with trypsin, the digests were eluted with 200 mM ammonium bicarbonate at pH 8.2 for CZE-MS/MS analysis using 1 M acetic acid as the background electrolyte. This combination of basic elution and acidic background electrolytes results in both sample stacking and formation of a dynamic pH junction. 369 protein groups and 1274 peptides were identified from 50 ng of Xenopus laevis zygote homogenate, which is comparable with an offline sample preparation method, but the time required for sample preparation was decreased from over 24 h to less than 40 min. Dramatically improved performance was produced by coupling the reactor to a longer separation capillary (∼100 cm) and a Q Exactive HF mass spectrometer. 975 protein groups and 3749 peptides were identified from 50 ng of Xenopus protein using the online sample preparation method.
Kelstrup,
Rapid and deep proteomes by faster sequencing on a benchtop quadrupole ultra-high-field Orbitrap mass spectrometer.
2014, Pubmed
Kelstrup,
Rapid and deep proteomes by faster sequencing on a benchtop quadrupole ultra-high-field Orbitrap mass spectrometer.
2014,
Pubmed
Krylov,
Instrumentation for chemical cytometry.
2000,
Pubmed
Li,
Capillary zone electrophoresis-electrospray ionization-tandem mass spectrometry as an alternative proteomics platform to ultraperformance liquid chromatography-electrospray ionization-tandem mass spectrometry for samples of intermediate complexity.
2012,
Pubmed
Liu,
Monolithic capillary column based glycoproteomic reactor for high-sensitive analysis of N-glycoproteome.
2013,
Pubmed
Ma,
Coupling formic acid assisted solubilization and online immobilized pepsin digestion with strong cation exchange and microflow reversed-phase liquid chromatography with electrospray ionization tandem mass spectrometry for integral membrane proteome analysis.
2010,
Pubmed
Ma,
Online integration of multiple sample pretreatment steps involving denaturation, reduction, and digestion with microflow reversed-phase liquid chromatography-electrospray ionization tandem mass spectrometry for high-throughput proteome profiling.
2009,
Pubmed
Scheltema,
The Q Exactive HF, a Benchtop mass spectrometer with a pre-filter, high-performance quadrupole and an ultra-high-field Orbitrap analyzer.
2014,
Pubmed
Smith,
Measurement of protein using bicinchoninic acid.
1985,
Pubmed
Sun,
Over 10,000 peptide identifications from the HeLa proteome by using single-shot capillary zone electrophoresis combined with tandem mass spectrometry.
2014,
Pubmed
Sun,
Quantitative proteomics of Xenopus laevis embryos: expression kinetics of nearly 4000 proteins during early development.
2014,
Pubmed
,
Xenbase
Sun,
Integrated capillary zone electrophoresis-electrospray ionization tandem mass spectrometry system with an immobilized trypsin microreactor for online digestion and analysis of picogram amounts of RAW 264.7 cell lysate.
2013,
Pubmed
Tian,
Rare cell proteomic reactor applied to stable isotope labeling by amino acids in cell culture (SILAC)-based quantitative proteomics study of human embryonic stem cell differentiation.
2011,
Pubmed
Wang,
Improving the comprehensiveness and sensitivity of sheathless capillary electrophoresis-tandem mass spectrometry for proteomic analysis.
2012,
Pubmed
Wojcik,
Simplified capillary electrophoresis nanospray sheath-flow interface for high efficiency and sensitive peptide analysis.
2010,
Pubmed
Zhang,
Integrated strong cation-exchange hybrid monolith coupled with capillary zone electrophoresis and simultaneous dynamic pH junction for large-volume proteomic analysis by mass spectrometry.
2015,
Pubmed
Zhang,
Preparation of capillary hybrid monolithic column with sulfonate strong cation exchanger for proteome analysis.
2012,
Pubmed
Zhang,
Detachable strong cation exchange monolith, integrated with capillary zone electrophoresis and coupled with pH gradient elution, produces improved sensitivity and numbers of peptide identifications during bottom-up analysis of complex proteomes.
2015,
Pubmed
Zhao,
Capillary electrophoresis-mass spectrometry for analysis of complex samples.
2012,
Pubmed
Zhou,
Analysis of the subcellular phosphoproteome using a novel phosphoproteomic reactor.
2010,
Pubmed
Zhu,
Single-shot proteomics using capillary zone electrophoresis-electrospray ionization-tandem mass spectrometry with production of more than 1250 Escherichia coli peptide identifications in a 50 min separation.
2013,
Pubmed
