Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
Proc Natl Acad Sci U S A
2003 Mar 04;1005:2935-40. doi: 10.1073/pnas.0636603100.
Show Gene links
Show Anatomy links
Structural basis of two-stage voltage-dependent activation in K+ channels.
Silverman WR
,
Roux B
,
Papazian DM
.
???displayArticle.abstract???
The structure of the voltage sensor and the detailed physical basis of voltage-dependent activation in ion channels have not been determined. We now have identified conserved molecular rearrangements underlying two major voltage-dependent conformational changes during activation of divergent K(+) channels, ether-à-go-go (eag) and Shaker. Two conserved arginines of the S4 voltage sensor move sequentially into an extracellular gating pocket, where they interact with an acidic residue in S2. In eag, these transitions are modulated by a divalent ion that binds in the gating pocket. Conservation of key molecular details in the activation mechanism confirms that voltage sensors in divergent K(+) channels share a common structure. Molecular modeling reveals that structural constraints derived from eag and Shaker specify the unique packing arrangement of transmembrane segments S2, S3, and S4 within the voltage sensor.
Aggarwal,
Contribution of the S4 segment to gating charge in the Shaker K+ channel.
1996, Pubmed,
Xenbase
Aggarwal,
Contribution of the S4 segment to gating charge in the Shaker K+ channel.
1996,
Pubmed
,
Xenbase
Bezanilla,
Gating of Shaker K+ channels: II. The components of gating currents and a model of channel activation.
1994,
Pubmed
,
Xenbase
Blaustein,
Tethered blockers as molecular 'tape measures' for a voltage-gated K+ channel.
2000,
Pubmed
,
Xenbase
Cestèle,
Molecular mechanisms of neurotoxin action on voltage-gated sodium channels.
2000,
Pubmed
Cha,
Atomic scale movement of the voltage-sensing region in a potassium channel measured via spectroscopy.
1999,
Pubmed
Elinder,
S4 charges move close to residues in the pore domain during activation in a K channel.
2001,
Pubmed
,
Xenbase
Hong,
The lipid-protein interface of a Shaker K(+) channel.
2000,
Pubmed
,
Xenbase
Li-Smerin,
A localized interaction surface for voltage-sensing domains on the pore domain of a K+ channel.
2000,
Pubmed
,
Xenbase
Li-Smerin,
Localization and molecular determinants of the Hanatoxin receptors on the voltage-sensing domains of a K(+) channel.
2000,
Pubmed
,
Xenbase
Li-Smerin,
Gating modifier toxins reveal a conserved structural motif in voltage-gated Ca2+ and K+ channels.
1998,
Pubmed
,
Xenbase
Li-Smerin,
alpha-helical structural elements within the voltage-sensing domains of a K(+) channel.
2000,
Pubmed
,
Xenbase
Lu,
Ion conduction pore is conserved among potassium channels.
2001,
Pubmed
MacKinnon,
Structural conservation in prokaryotic and eukaryotic potassium channels.
1998,
Pubmed
Monks,
Helical structure and packing orientation of the S2 segment in the Shaker K+ channel.
1999,
Pubmed
,
Xenbase
Montal,
Molecular anatomy and molecular design of channel proteins.
1990,
Pubmed
Papazian,
Electrostatic interactions of S4 voltage sensor in Shaker K+ channel.
1995,
Pubmed
,
Xenbase
Roux,
What can be deduced about the structure of Shaker from available data?
2002,
Pubmed
Seoh,
Voltage-sensing residues in the S2 and S4 segments of the Shaker K+ channel.
1996,
Pubmed
,
Xenbase
Silverman,
Mg(2+) modulates voltage-dependent activation in ether-à-go-go potassium channels by binding between transmembrane segments S2 and S3.
2000,
Pubmed
,
Xenbase
Sokolova,
Three-dimensional structure of a voltage-gated potassium channel at 2.5 nm resolution.
2001,
Pubmed
Starace,
Histidine scanning mutagenesis of basic residues of the S4 segment of the shaker k+ channel.
2001,
Pubmed
,
Xenbase
Starace,
Voltage-dependent proton transport by the voltage sensor of the Shaker K+ channel.
1997,
Pubmed
Takahashi,
Solution structure of hanatoxin1, a gating modifier of voltage-dependent K(+) channels: common surface features of gating modifier toxins.
2000,
Pubmed
,
Xenbase
Tang,
Extracellular Mg(2+) modulates slow gating transitions and the opening of Drosophila ether-à-Go-Go potassium channels.
2000,
Pubmed
,
Xenbase
Terlau,
Extracellular Mg2+ regulates activation of rat eag potassium channel.
1996,
Pubmed
,
Xenbase
Tiwari-Woodruff,
Voltage-dependent structural interactions in the Shaker K(+) channel.
2000,
Pubmed
,
Xenbase
Tiwari-Woodruff,
Electrostatic interactions between transmembrane segments mediate folding of Shaker K+ channel subunits.
1997,
Pubmed
,
Xenbase
Zagotta,
Shaker potassium channel gating. III: Evaluation of kinetic models for activation.
1994,
Pubmed
,
Xenbase
Zhou,
Chemistry of ion coordination and hydration revealed by a K+ channel-Fab complex at 2.0 A resolution.
2001,
Pubmed
