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XB-ART-54439
Cold Spring Harb Protoc 2018 Oct 01;201810:. doi: 10.1101/pdb.prot096982.
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Oocyte Host-Transfer and Maternal mRNA Depletion Experiments in Xenopus.



Abstract
This protocol details the oocyte host-transfer method in Xenopus, using transplantation by intraperitoneal injection. This approach is suitable for the overexpression of mRNAs and for the use of antisense oligonucleotides to deplete maternal mRNAs, which are not replaced until zygotic genome activation in the mid-blastula transition. Xenopus oocyte host-transfer can also be used for highly efficient mutagenesis in the F0 generation by prefertilization injection of genome editing reagents.

PubMed ID: 29321286
PMC ID: PMC6538067
Article link: Cold Spring Harb Protoc
Grant support: [+]


References [+] :
ARNOLD, Interfemale transfer of eggs and ovaries in the frog. 1962, Pubmed