Cuykendall TN , Houston DW .

5R01 GM083999-02 NIGMS NIH HHS , R01 GM083999-02 NIGMS NIH HHS , R01 GM083999 NIGMS NIH HHS

	Fig. 1. Expression of trim36 in Xenopus. (A) RT-PCR for trim36 at different Nieuwkoop and Faber (NF) stages; `-RT' was processed in the absence of reverse transcriptase. ornithine decarboxylase (odc) was included as a loading control. (B-E) Whole-mount in situs using antisense trim36 probe. (B) Stage I oocytes, (C) stage VI (left) and stage IV (right) oocytes, (D) 4-cell embryos (vegetal view) and (E) neurula embryos (dorsal/anterior view). (F-H) In situs for trim36 on sections; insets in F and G are low-power views, inset in H is trim36 sense probe. (F) Stage 7 sagittal section, (G) stage 11 sagittal section and (H) adult testis.
	Fig. 3. Dorsal marker expression in trim36-depleted embryos. Dorsal genes and β-catenin protein in control (A,C,E,G) and trim36-depleted (B,D,F,H) embryos. (A,B) In situs for eomes in stage 12 embryos. Dorsal view, anterior is to the top. Arrow in A indicates eomes expression in the anterior notochord. (C,D) In situs for myod in stage 12 embryos. Dorsal view, anterior is to the top. (E,F) In situs for nr3 in stage 9.5 embryos. Vegetal view, dorsal is to the top. (G,H) Immunostaining for β-catenin in stage 8 embryos. Animal pole view of cleared embryos, dorsal is to the right. Arrow in G indicates nuclearβ -catenin. (I) Quantitative real-time PCR of dorsal (sia and nr3) and ventral (szl) markers in control (Un) and trim36-depleted (trim36-) embryos at stage 10.5.
	Fig. 5. trim36-depleted embryos are rescued by wnt11 mRNA.trim36 was depleted by the injection of antisense oligos into oocytes and 50 pg wnt11 mRNA was injected prior to fertilization by the host-transfer method. gsc (A,C,E) and nr3 (B,D,F) expression in stage 10.5 embryos; (A,B) uninjected; (C,D) trim36-depleted (trim36-); and (E,F) trim36-depleted + 50 pg wnt11 mRNA (trim36-; + wnt11). (G) Histogram showing the distribution of phenotypes (see key) in uninjected embryos, trim-36-depleted embryos and trim36-depleted embryos rescued by wnt11 injection (from two experiments). (H,I) Quantitative real-time PCR analysis of nr3 expression in control, depleted and rescued embyros injected with 50 pg wnt11 (H) or 1.0 ng dngsk3b (I) (green bars).

Belenkaya, pygopus Encodes a nuclear protein essential for wingless/Wnt signaling. 2002, Pubmed, Xenbase

Belenkaya, pygopus Encodes a nuclear protein essential for wingless/Wnt signaling. 2002, Pubmed , Xenbase
Brown, Provisional bilateral symmetry in Xenopus eggs is established during maturation. 1994, Pubmed , Xenbase
Chan, The maternally localized RNA fatvg is required for cortical rotation and germ cell formation. 2007, Pubmed , Xenbase
Collavin, The secreted Frizzled-related protein Sizzled functions as a negative feedback regulator of extreme ventral mesoderm. 2003, Pubmed , Xenbase
Darras, Animal and vegetal pole cells of early Xenopus embryos respond differently to maternal dorsal determinants: implications for the patterning of the organiser. 1997, Pubmed , Xenbase
Elinson, A transient array of parallel microtubules in frog eggs: potential tracks for a cytoplasmic rotation that specifies the dorso-ventral axis. 1988, Pubmed , Xenbase
Elinson, Isolated vegetal cortex from Xenopus oocytes selectively retains localized mRNAs. 1993, Pubmed , Xenbase
Eyal-Giladi, Establishment of the axis in chordates: facts and speculations. 1997, Pubmed
Gerhart, Cortical rotation of the Xenopus egg: consequences for the anteroposterior pattern of embryonic dorsal development. 1989, Pubmed , Xenbase
Godsave, Oocytes and early embryos of Xenopus laevis contain intermediate filaments which react with anti-mammalian vimentin antibodies. 1984, Pubmed , Xenbase
Heasman, Vegetal localization of maternal mRNAs is disrupted by VegT depletion. 2001, Pubmed , Xenbase
Heasman, The mitochondrial cloud of Xenopus oocytes: the source of germinal granule material. 1984, Pubmed , Xenbase
Heasman, Fertilization of cultured Xenopus oocytes and use in studies of maternally inherited molecules. 1991, Pubmed , Xenbase
Heasman, Overexpression of cadherins and underexpression of beta-catenin inhibit dorsal mesoderm induction in early Xenopus embryos. 1994, Pubmed , Xenbase
Houliston, Evidence for the involvement of microtubules, ER, and kinesin in the cortical rotation of fertilized frog eggs. 1991, Pubmed , Xenbase
Houston, Repression of organizer genes in dorsal and ventral Xenopus cells mediated by maternal XTcf3. 2002, Pubmed , Xenbase
Houston, The Xenopus LIM-homeodomain protein Xlim5 regulates the differential adhesion properties of early ectoderm cells. 2003, Pubmed , Xenbase
Houston, Maternal Xenopus Zic2 negatively regulates Nodal-related gene expression during anteroposterior patterning. 2005, Pubmed , Xenbase
Houston, A critical role for Xdazl, a germ plasm-localized RNA, in the differentiation of primordial germ cells in Xenopus. 2000, Pubmed , Xenbase
Houston, Germ plasm and molecular determinants of germ cell fate. 2000, Pubmed , Xenbase
Javanbakht, The contribution of RING and B-box 2 domains to retroviral restriction mediated by monkey TRIM5alpha. 2005, Pubmed
Kageura, Activation of dorsal development by contact between the cortical dorsal determinant and the equatorial core cytoplasm in eggs of Xenopus laevis. 1997, Pubmed , Xenbase
Kerr, Maternal Tgif1 regulates nodal gene expression in Xenopus. 2008, Pubmed , Xenbase
Kitamura, Haprin, a novel haploid germ cell-specific RING finger protein involved in the acrosome reaction. 2003, Pubmed
Kitamura, The RING-finger protein haprin: domains and function in the acrosome reaction. 2005, Pubmed
Kloc, Two distinct pathways for the localization of RNAs at the vegetal cortex in Xenopus oocytes. 1995, Pubmed , Xenbase
Kloc, Potential structural role of non-coding and coding RNAs in the organization of the cytoskeleton at the vegetal cortex of Xenopus oocytes. 2005, Pubmed , Xenbase
Klymkowsky, Polar asymmetry in the organization of the cortical cytokeratin system of Xenopus laevis oocytes and embryos. 1987, Pubmed , Xenbase
Kofron, The role of maternal axin in patterning the Xenopus embryo. 2001, Pubmed , Xenbase
Ku, Xwnt-11: a maternally expressed Xenopus wnt gene. 1993, Pubmed , Xenbase
Lee, Embryonic dorsal-ventral signaling: secreted frizzled-related proteins as inhibitors of tolloid proteinases. 2006, Pubmed , Xenbase
Marikawa, Dorsal determinants in the Xenopus egg are firmly associated with the vegetal cortex and behave like activators of the Wnt pathway. 1997, Pubmed , Xenbase
McMahon, Ectopic expression of the proto-oncogene int-1 in Xenopus embryos leads to duplication of the embryonic axis. 1989, Pubmed , Xenbase
Medina, Cortical rotation is required for the correct spatial expression of nr3, sia and gsc in Xenopus embryos. 1997, Pubmed , Xenbase
Meroni, TRIM/RBCC, a novel class of 'single protein RING finger' E3 ubiquitin ligases. 2005, Pubmed
Miller, Establishment of the dorsal-ventral axis in Xenopus embryos coincides with the dorsal enrichment of dishevelled that is dependent on cortical rotation. 1999, Pubmed , Xenbase
Miyajima, TRIM36 interacts with the kinetochore protein CENP-H and delays cell cycle progression. 2009, Pubmed
Quaas, Surface contraction waves (SCWs) in the Xenopus egg are required for the localization of the germ plasm and are dependent upon maternal stores of the kinesin-like protein Xklp1. 2002, Pubmed , Xenbase
Salic, Sizzled: a secreted Xwnt8 antagonist expressed in the ventral marginal zone of Xenopus embryos. 1997, Pubmed , Xenbase
Savage, Dynamics of germ plasm localization and its inhibition by ultraviolet irradiation in early cleavage Xenopus embryos. 1993, Pubmed , Xenbase
Scharf, Determination of the dorsal-ventral axis in eggs of Xenopus laevis: complete rescue of uv-impaired eggs by oblique orientation before first cleavage. 1980, Pubmed , Xenbase
Scharf, Axis determination in eggs of Xenopus laevis: a critical period before first cleavage, identified by the common effects of cold, pressure and ultraviolet irradiation. 1983, Pubmed , Xenbase
Schneider, Beta-catenin translocation into nuclei demarcates the dorsalizing centers in frog and fish embryos. 1996, Pubmed , Xenbase
Short, Subclassification of the RBCC/TRIM superfamily reveals a novel motif necessary for microtubule binding. 2006, Pubmed
Smith, A nodal-related gene defines a physical and functional domain within the Spemann organizer. 1995, Pubmed , Xenbase
Sokol, Dorsalizing and neuralizing properties of Xdsh, a maternally expressed Xenopus homolog of dishevelled. 1995, Pubmed , Xenbase
Sumanas, The putative wnt receptor Xenopus frizzled-7 functions upstream of beta-catenin in vertebrate dorsoventral mesoderm patterning. 2000, Pubmed , Xenbase
Tao, Maternal wnt11 activates the canonical wnt signaling pathway required for axis formation in Xenopus embryos. 2005, Pubmed , Xenbase
Trockenbacher, MID1, mutated in Opitz syndrome, encodes an ubiquitin ligase that targets phosphatase 2A for degradation. 2001, Pubmed
Vincent, Kinematics of gray crescent formation in Xenopus eggs: the displacement of subcortical cytoplasm relative to the egg surface. 1986, Pubmed , Xenbase
Weaver, GBP binds kinesin light chain and translocates during cortical rotation in Xenopus eggs. 2003, Pubmed , Xenbase
Weaver, Move it or lose it: axis specification in Xenopus. 2004, Pubmed , Xenbase
Yokota, A novel role for a nodal-related protein; Xnr3 regulates convergent extension movements via the FGF receptor. 2003, Pubmed , Xenbase
Yoshigai, Trim36/Haprin plays a critical role in the arrangement of somites during Xenopus embryogenesis. 2009, Pubmed , Xenbase
Yost, The axis-inducing activity, stability, and subcellular distribution of beta-catenin is regulated in Xenopus embryos by glycogen synthase kinase 3. 1996, Pubmed , Xenbase
Yost, GBP, an inhibitor of GSK-3, is implicated in Xenopus development and oncogenesis. 1998, Pubmed , Xenbase