Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
Xenopus laevis ribosomal protein genes: isolation of recombinant cDNA clones and study of the genomic organization.
Bozzoni I
,
Beccari E
,
Luo ZX
,
Amaldi F
.
???displayArticle.abstract???
Poly-A+ mRNA from Xenopus laevis oocytes, partially enriched for r-protein coding capacity has been used as starting material for preparing a cDNA bank in plasmid pBR322. The clones containing sequences specific for r-proteins have been selected by translation of the complementary mRNAs. Clones for six different r-proteins have been identified and utilized as probes for studying their genomic organization. Two gene copies per haploid genome were found for r-proteins L1, L14, S19, and four-five for protein S1, S8 and L32. Moreover a population polymorphism has been observed for the genomic regions containing sequences for r-protein S1, S8 and L14.
Cleveland,
Peptide mapping by limited proteolysis in sodium dodecyl sulfate and analysis by gel electrophoresis.
1977, Pubmed
Cleveland,
Peptide mapping by limited proteolysis in sodium dodecyl sulfate and analysis by gel electrophoresis.
1977,
Pubmed
Efstratiadis,
Full length and discrete partial reverse transcripts of globin and chorion mRNAs.
1975,
Pubmed
Fantoni,
Construction of a recombinant bacterial plasmid containing DNA sequences for a mouse embryonic globin chain.
1979,
Pubmed
Gorenstein,
Coordinate regulation of the synthesis of eukaryotic ribosomal proteins.
1976,
Pubmed
Grunstein,
Colony hybridization: a method for the isolation of cloned DNAs that contain a specific gene.
1975,
Pubmed
Laskey,
Quantitative film detection of 3H and 14C in polyacrylamide gels by fluorography.
1975,
Pubmed
Loening,
The fractionation of high-molecular-weight ribonucleic acid by polyacrylamide-gel electrophoresis.
1967,
Pubmed
Maniatis,
Amplification and characterization of a beta-globin gene synthesized in vitro.
1976,
Pubmed
Meyuhas,
Construction and identification of cDNA clones for mouse ribosomal proteins: application for the study of r-protein gene expression.
1980,
Pubmed
Pierandrei-Amaldi,
Messenger RNA for ribosomal proteins in Xenopus laevis oocytes.
1980,
Pubmed
,
Xenbase
Southern,
Detection of specific sequences among DNA fragments separated by gel electrophoresis.
1975,
Pubmed
Vaslet,
Isolation and mapping of a cloned ribosomal protein gene of Drosophila melanogaster.
1980,
Pubmed
Woolford,
Isolation of cloned DNA sequences containing ribosomal protein genes from Saccharomyces cerevisiae.
1979,
Pubmed
Zasloff,
A new method for the purification and identification of covalently closed circular DNA molcules.
1978,
Pubmed
