Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
EMBO J
1990 Jun 01;96:1749-56. doi: 10.1002/j.1460-2075.1990.tb08299.x.
Show Gene links
Show Anatomy links
Cloning and expression of a human voltage-gated potassium channel. A novel member of the RCK potassium channel family.
Grupe A
,
Schröter KH
,
Ruppersberg JP
,
Stocker M
,
Drewes T
,
Beckh S
,
Pongs O
.
Abstract
We have isolated and characterized a human cDNA (HBK2) that is homologous to novel member (RCK2) of the K+ channel RCK gene family expressed in rat brain. RCK2 mRNA was detected predominantly in midbrain areas and brainstem. The primary sequences of the HBK2/RCK2 K+ channel proteins exhibit major differences to other members of the RCK gene family. The bend region between segments S1 and S2 is unusually long and does not contain the N-glycosylation site commonly found in this region. They might be O-glycosylated instead. Functional characterization of the HBK2/RCK2 K+ channels in Xenopus laevis oocytes following micro-injection in in vitro transcribed HBK2 or RCK2 cRNA showed that the HBK2/RCK2 proteins form voltage-gated K+ channels with novel functional and pharmacological properties. These channels are different to RCK1, RCK3, RCK4 and RCK5 K+ channels.
Baumann,
Structure of the voltage-dependent potassium channel is highly conserved from Drosophila to vertebrate central nervous systems.
1988, Pubmed
Baumann,
Structure of the voltage-dependent potassium channel is highly conserved from Drosophila to vertebrate central nervous systems.
1988,
Pubmed
Beckh,
Differential regulation of three sodium channel messenger RNAs in the rat central nervous system during development.
1989,
Pubmed
Beckh,
Members of the RCK potassium channel family are differentially expressed in the rat nervous system.
1990,
Pubmed
Benton,
Screening lambdagt recombinant clones by hybridization to single plaques in situ.
1977,
Pubmed
Black,
Two acceptor sub-types for dendrotoxin in chick synaptic membranes distinguishable by beta-bungarotoxin.
1986,
Pubmed
Butler,
A family of putative potassium channel genes in Drosophila.
1989,
Pubmed
Chirgwin,
Isolation of biologically active ribonucleic acid from sources enriched in ribonuclease.
1979,
Pubmed
Frech,
A novel potassium channel with delayed rectifier properties isolated from rat brain by expression cloning.
1989,
Pubmed
,
Xenbase
Iverson,
A-type potassium channels expressed from Shaker locus cDNA.
1988,
Pubmed
,
Xenbase
Kamb,
Multiple products of the Drosophila Shaker gene may contribute to potassium channel diversity.
1988,
Pubmed
Kamb,
Identification of genes from pattern formation, tyrosine kinase, and potassium channel families by DNA amplification.
1989,
Pubmed
Kyte,
A simple method for displaying the hydropathic character of a protein.
1982,
Pubmed
Lin,
An improved DNA sequencing strategy.
1985,
Pubmed
MacKinnon,
Mutant potassium channels with altered binding of charybdotoxin, a pore-blocking peptide inhibitor.
1989,
Pubmed
,
Xenbase
McGinnis,
A conserved DNA sequence in homoeotic genes of the Drosophila Antennapedia and bithorax complexes.
,
Pubmed
McKinnon,
Isolation of a cDNA clone coding for a putative second potassium channel indicates the existence of a gene family.
1989,
Pubmed
,
Xenbase
Pongs,
Shaker encodes a family of putative potassium channel proteins in the nervous system of Drosophila.
1988,
Pubmed
Rehm,
Dendrotoxin-binding brain membrane protein displays a K+ channel activity that is stimulated by both cAMP-dependent and endogenous phosphorylations.
1989,
Pubmed
Rehm,
Purification and subunit structure of a putative K+-channel protein identified by its binding properties for dendrotoxin I.
1988,
Pubmed
Rudy,
Diversity and ubiquity of K channels.
1988,
Pubmed
Sanger,
DNA sequencing with chain-terminating inhibitors.
1977,
Pubmed
Schwarz,
Multiple potassium-channel components are produced by alternative splicing at the Shaker locus in Drosophila.
1988,
Pubmed
Shull,
Molecular cloning of three distinct forms of the Na+,K+-ATPase alpha-subunit from rat brain.
1986,
Pubmed
Stühmer,
Molecular basis of functional diversity of voltage-gated potassium channels in mammalian brain.
1989,
Pubmed
,
Xenbase
Stühmer,
Patch clamp characterization of sodium channels expressed from rat brain cDNA.
1987,
Pubmed
,
Xenbase
Stühmer,
Potassium channels expressed from rat brain cDNA have delayed rectifier properties.
1988,
Pubmed
,
Xenbase
Stühmer,
Structural parts involved in activation and inactivation of the sodium channel.
1989,
Pubmed
,
Xenbase
Tempel,
Cloning of a probable potassium channel gene from mouse brain.
1988,
Pubmed
Thomas,
Hybridization of denatured RNA and small DNA fragments transferred to nitrocellulose.
1980,
Pubmed
Timpe,
Four cDNA clones from the Shaker locus of Drosophila induce kinetically distinct A-type potassium currents in Xenopus oocytes.
1988,
Pubmed
,
Xenbase
Timpe,
Expression of functional potassium channels from Shaker cDNA in Xenopus oocytes.
1988,
Pubmed
,
Xenbase
Vogelstein,
Preparative and analytical purification of DNA from agarose.
1979,
Pubmed
