Click here to close Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly. We suggest using a current version of Chrome, FireFox, or Safari.
XB-ART-13820
J Cell Biol 1998 Dec 14;1436:1437-46.
Show Gene links Show Anatomy links

pEg7, a new Xenopus protein required for mitotic chromosome condensation in egg extracts.

Cubizolles F , Legagneux V , Le Guellec R , Chartrain I , Uzbekov R , Ford C , Le Guellec K .


???displayArticle.abstract???
We have isolated a cDNA, Eg7, corresponding to a Xenopus maternal mRNA, which is polyadenylated in mature oocytes and deadenylated in early embryos. This maternal mRNA encodes a protein, pEg7, whose expression is strongly increased during oocyte maturation. The tissue and cell expression pattern of pEg7 indicates that this protein is only readily detected in cultured cells and germ cells. Immunolocalization in Xenopus cultured cells indicates that pEg7 concentrates onto chromosomes during mitosis. A similar localization of pEg7 is observed when sperm chromatin is allowed to form mitotic chromosomes in cytostatic factor-arrested egg extracts. Incubating these extracts with antibodies directed against two distinct parts of pEg7 provokes a strong inhibition of the condensation and resolution of mitotic chromosomes. Biochemical experiments show that pEg7 associates with Xenopus chromosome-associated polypeptides C and E, two components of the 13S condensin.

???displayArticle.pubmedLink??? 9852142
???displayArticle.pmcLink??? PMC2132990



Species referenced: Xenopus
Genes referenced: ncapd2


???attribute.lit??? ???displayArticles.show???
References [+] :
Adachi, Chromosome assembly in vitro: topoisomerase II is required for condensation. 1991, Pubmed, Xenbase