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Methods Mol Biol
2016 Jan 01;1377:281-91. doi: 10.1007/978-1-4939-3179-8_25.
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Voltage Clamp Fluorometry of P-Type ATPases.
Dempski RE
.
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Voltage clamp fluorometry has become a powerful tool to compare partial reactions of P-type ATPases such as the Na(+),K(+)-ATPase and H(+),K(+)-ATPase with conformational dynamics of these ion pumps. Here, we describe the methodology to heterologously express membrane proteins in X. laevis oocytes and site-specifically label these proteins with one or more fluorophores. Fluorescence changes are measured simultaneously with current measurements under two-electrode voltage clamp conditions.
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