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Faithful expression of a tagged Fugu WT1 protein from a genomic transgene in zebrafish: efficient splicing of pufferfish genes in zebrafish but not mice.
Miles CG
,
Rankin L
,
Smith SI
,
Niksic M
,
Elgar G
,
Hastie ND
.
Abstract
The teleost fish are widely used as model organisms in vertebrate biology. The compact genome of the pufferfish, Fugu rubripes, has proven a valuable tool in comparative genome analyses, aiding the annotation of mammalian genomes and the identification of conserved regulatory elements, whilst the zebrafish is particularly suited to genetic and developmental studies. We demonstrate that a pufferfish WT1 transgene can be expressed and spliced appropriately in transgenic zebrafish, contrasting with the situation in transgenic mice. By creating both transgenic mice and transgenic zebrafish with the same construct, we show that Fugu RNA is processed correctly in zebrafish but not in mice. Furthermore, we show for the first time that a Fugu genomic construct can produce protein in transgenic zebrafish: a full-length Fugu WT1 transgene with a C-terminal beta-galactosidase fusion is spliced and translated correctly in zebrafish, mimicking the expression of the endogenous WT1 gene. These data demonstrate that the zebrafish:Fugu system is a powerful and convenient tool for dissecting both vertebrate gene regulation and gene function in vivo.
Amsterdam,
The Aequorea victoria green fluorescent protein can be used as a reporter in live zebrafish embryos.
1995, Pubmed,
Xenbase
Amsterdam,
The Aequorea victoria green fluorescent protein can be used as a reporter in live zebrafish embryos.
1995,
Pubmed
,
Xenbase
Aparicio,
Detecting conserved regulatory elements with the model genome of the Japanese puffer fish, Fugu rubripes.
1995,
Pubmed
Armstrong,
The expression of the Wilms' tumour gene, WT1, in the developing mammalian embryo.
1993,
Pubmed
Ars,
Cold shock induces the insertion of a cryptic exon in the neurofibromatosis type 1 (NF1) mRNA.
2000,
Pubmed
Barbaux,
Donor splice-site mutations in WT1 are responsible for Frasier syndrome.
1997,
Pubmed
Barton,
Regulation of the stem cell leukemia (SCL) gene: a tale of two fishes.
2001,
Pubmed
Baxendale,
Comparative sequence analysis of the human and pufferfish Huntington's disease genes.
1995,
Pubmed
Berget,
Exon recognition in vertebrate splicing.
1995,
Pubmed
Brenner,
Characterization of the pufferfish (Fugu) genome as a compact model vertebrate genome.
1993,
Pubmed
Broadbent,
Wholemount in situ hybridization of Xenopus and zebrafish embryos.
1999,
Pubmed
,
Xenbase
Call,
Isolation and characterization of a zinc finger polypeptide gene at the human chromosome 11 Wilms' tumor locus.
1990,
Pubmed
Davies,
Did nucleotides or amino acids drive evolutionary conservation of the WT1 +/-KTS alternative splice?
2000,
Pubmed
Drummond,
Early development of the zebrafish pronephros and analysis of mutations affecting pronephric function.
1998,
Pubmed
Elgar,
Small is beautiful: comparative genomics with the pufferfish (Fugu rubripes).
1996,
Pubmed
Elgar,
Generation and analysis of 25 Mb of genomic DNA from the pufferfish Fugu rubripes by sequence scanning.
1999,
Pubmed
Englert,
WT1--more than a transcription factor?
1998,
Pubmed
Gessler,
Homozygous deletion in Wilms tumours of a zinc-finger gene identified by chromosome jumping.
1990,
Pubmed
Glaser,
A mouse model of the aniridia-Wilms tumor deletion syndrome.
1990,
Pubmed
Hastie,
Life, sex, and WT1 isoforms--three amino acids can make all the difference.
2001,
Pubmed
Hastie,
The genetics of Wilms' tumor--a case of disrupted development.
1994,
Pubmed
Kent,
The reticulocalbin gene maps to the WAGR region in human and to the Small eye Harwell deletion in mouse.
1997,
Pubmed
Kent,
The evolution of WT1 sequence and expression pattern in the vertebrates.
1995,
Pubmed
,
Xenbase
Kreidberg,
WT-1 is required for early kidney development.
1993,
Pubmed
Lander,
Initial sequencing and analysis of the human genome.
2001,
Pubmed
Lee,
Wilms tumor and the WT1 gene.
2001,
Pubmed
Menke,
The Wilms' tumor suppressor WT1: approaches to gene function.
1998,
Pubmed
Miles,
Complete sequencing of the Fugu WAGR region from WT1 to PAX6: dramatic compaction and conservation of synteny with human chromosome 11p13.
1998,
Pubmed
,
Xenbase
Modrek,
A genomic view of alternative splicing.
2002,
Pubmed
Moore,
YAC complementation shows a requirement for Wt1 in the development of epicardium, adrenal gland and throughout nephrogenesis.
1999,
Pubmed
Moore,
YAC transgenic analysis reveals Wilms' tumour 1 gene activity in the proliferating coelomic epithelium, developing diaphragm and limb.
1998,
Pubmed
Nakai,
Construction of a novel database containing aberrant splicing mutations of mammalian genes.
1994,
Pubmed
Nonchev,
The conserved role of Krox-20 in directing Hox gene expression during vertebrate hindbrain segmentation.
1996,
Pubmed
Pelletier,
Germline mutations in the Wilms' tumor suppressor gene are associated with abnormal urogenital development in Denys-Drash syndrome.
1991,
Pubmed
Pritchard-Jones,
The candidate Wilms' tumour gene is involved in genitourinary development.
1990,
Pubmed
Reed,
A role for exon sequences and splice-site proximity in splice-site selection.
1986,
Pubmed
Sathasivam,
Aberrant processing of the Fugu HD (FrHD) mRNA in mouse cells and in transgenic mice.
1997,
Pubmed
Schmitt,
Alternative splicing of E1A transcripts of adenovirus requires appropriate ionic conditions in vitro.
1987,
Pubmed
Semba,
cDNA cloning and its pronephros-specific expression of the Wilms' tumor suppressor gene, WT1, from Xenopus laevis.
1996,
Pubmed
,
Xenbase
Sterner,
Architectural limits on split genes.
1996,
Pubmed
Venter,
The sequence of the human genome.
2001,
Pubmed
Weil,
Temperature-dependent expression of a collagen splicing defect in the fibroblasts of a patient with Ehlers-Danlos syndrome type VII.
1989,
Pubmed
Zhou,
Wilms' tumor (WT1) gene expression in rat decidual differentiation.
1993,
Pubmed
