Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
In vitro transcription of a cloned mouse ribosomal RNA gene.
Mishima Y
,
Yamamoto O
,
Kominami R
,
Muramatsu M
.
Abstract
An in vitro transcription system which utilizes cloned mouse ribosomal RNA gene (rDNA) fragments and a mouse cell extract has been developed. RNA polymerases I is apparently responsible for this transcription as evidenced by the complete resistance to a high concentration (200 micrograms/ml) of alpha-amanitin. Run-off products obtained with three different truncated rDNA fragments indicated that RNA was transcribed from a unique site of rDNA. The S1 nuclease protection mapping of the in vitro product and of in vivo 45S RNA confirmed this site, indicating that, in this in vitro system, transcription of rDNA started from the same site as in vivo. This site is located at several hundred nucleotides upstream from the putative initiation site reported by us (1) and by others (2). Some sequence homology surrounding this region was noted among mouse, Xenopus laevis and Drosophila melanogaster. The data also suggest that some processing of the primary transcript occurs in this in vitro system.
Bach,
The nucleotide sequence of the initiation region of the ribosomal transcription unit from mouse.
1981, Pubmed
Bach,
The nucleotide sequence of the initiation region of the ribosomal transcription unit from mouse.
1981,
Pubmed
Berk,
Sizing and mapping of early adenovirus mRNAs by gel electrophoresis of S1 endonuclease-digested hybrids.
1977,
Pubmed
Bogenhagen,
A control region in the center of the 5S RNA gene directs specific initiation of transcription: II. The 3' border of the region.
1980,
Pubmed
,
Xenbase
Corden,
Promoter sequences of eukaryotic protein-coding genes.
1980,
Pubmed
Currier,
Isolation of covalently closed circular DNA of high molecular weight from bacteria.
1976,
Pubmed
Grummt,
Specific transcription of mouse ribosomal DNA in a cell-free system that mimics control in vivo.
1981,
Pubmed
Kominami,
Heterogeneity of 5' -termini of nucleolar 45S, 32S and 28S RNA in mouse hepatoma.
1977,
Pubmed
Long,
Nucleotide sequence of the initiation site for ribosomal RNA transcription in Drosophila melanogaster: comparison of genes with and without insertions.
1981,
Pubmed
,
Xenbase
Manley,
DNA-dependent transcription of adenovirus genes in a soluble whole-cell extract.
1980,
Pubmed
Matsui,
Multiple factors required for accurate initiation of transcription by purified RNA polymerase II.
1980,
Pubmed
Maxam,
A new method for sequencing DNA.
1977,
Pubmed
Mishima,
The mechanism of decrease in nucleolar RNA synthesis by protein synthesis inhibition.
1979,
Pubmed
Mishima,
Cloning and determination of a putative promoter region of a mouse ribosomal deoxyribonucleic acid fragment.
1980,
Pubmed
Parker,
Selective and accurate transcription of the Xenopus laevis 5S RNA genes in isolated chromatin by purified RNA polymerase III.
1977,
Pubmed
,
Xenbase
Sakonju,
A control region in the center of the 5S RNA gene directs specific initiation of transcription: I. The 5' border of the region.
1980,
Pubmed
,
Xenbase
Segall,
Multiple factors are required for the accurate transcription of purified genes by RNA polymerase III.
1980,
Pubmed
Sollner-Webb,
The nucleotide sequence of the initiation and termination sites for ribosomal RNA transcription in X. laevis.
1979,
Pubmed
,
Xenbase
Tabak,
A method for the recovery of DNA from agarose gels.
1978,
Pubmed
Talkington,
In vitro transcription of normal, mutant, and truncated mouse alpha-globin genes.
1980,
Pubmed
Urano,
The nucleotide sequence of the putative transcription initiation site of a cloned ribosomal RNA gene of the mouse.
1980,
Pubmed
,
Xenbase
Wasylyk,
A T to A base substitution and small deletions in the conalbumin TATA box drastically decrease specific in vitro transcription.
1981,
Pubmed
Weil,
Faithful transcription of eukaryotic genes by RNA polymerase III in systems reconstituted with purified DNA templates.
1979,
Pubmed
,
Xenbase
Weil,
Selective and accurate initiation of transcription at the Ad2 major late promotor in a soluble system dependent on purified RNA polymerase II and DNA.
1979,
Pubmed
Wu,
Adenovirus DNA-directed transcription of 5.5S RNA in vitro.
1978,
Pubmed
Yamamoto,
Synthesis of ribosomal 5S RNA by isolated nuclei from HeLa cells in vitro.
1977,
Pubmed
Zimmerman,
The ribonuclease activity of crystallized pancreatic deoxyribonuclease.
1966,
Pubmed
