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XB-ART-29496
Proc Natl Acad Sci U S A 1984 Dec 01;8124:7907-11.
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Nucleotide sequence of human monocyte interleukin 1 precursor cDNA.

Auron PE , Webb AC , Rosenwasser LJ , Mucci SF , Rich A , Wolff SM , Dinarello CA .


Abstract
Interleukin 1 (IL-1) is a protein with several biological activities regulating host defense and immune responses. We report here the isolation of human IL-1 cDNA. It encodes a precursor polypeptide of 269 amino acids (30,747 Mr). mRNA isolated by hybridization to this cDNA was translated in a reticulocyte cell-free system, yielding immunoprecipitable IL-1. Furthermore, this hybrid-selected mRNA was injected into Xenopus laevis oocytes, which subsequently secreted biologically active IL-1. The cDNA nucleotide sequence suggests that IL-1 is initially translated as a precursor molecule that is subsequently processed into the 15,000-20,000 Mr protein usually associated with IL-1 activity.

PubMed ID: 6083565
PMC ID: PMC392262
Article link: Proc Natl Acad Sci U S A
Grant support: [+]

Species referenced: Xenopus laevis
Genes referenced: il1b

References [+] :
Biggin, Buffer gradient gels and 35S label as an aid to rapid DNA sequence determination. 1983, Pubmed