Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
Plant Physiol
2010 Mar 01;1523:1431-41. doi: 10.1104/pp.109.152009.
Show Gene links
Show Anatomy links
Channel-like characteristics of the low-affinity barley phosphate transporter PHT1;6 when expressed in Xenopus oocytes.
Preuss CP
,
Huang CY
,
Gilliham M
,
Tyerman SD
.
???displayArticle.abstract???
Remobilization of inorganic phosphate (P(i)) within a plant is critical for sustaining growth and seed production under external P(i) fluctuation. The barley (Hordeum vulgare) transporter HvPHT1;6 has been implicated in P(i) remobilization. In this report, we expressed HvPHT1;6 in Xenopus laevis oocytes, allowing detailed characterization of voltage-dependent fluxes and currents induced by HvPHT1;6. HvPHT1;6 increased efflux of P(i) near oocyte resting membrane potentials, dependent on external P(i) concentration. Time-dependent inward currents were observed when membrane potentials were more negative than -160 mV, which was consistent with nH(+):HPO(4)(2-) (n > 2) cotransport, based on simultaneous radiotracer and oocyte voltage clamping, dependent upon P(i) concentration gradient and pH. Time- and voltage-dependent inward currents through HvPHT1;6 were also observed for SO(4)(2-)and to a lesser degree for NO(3)(-)Cl(-)but not for malate. Inward and outward currents showed linear dependence on the concentration of external HPO(4)(2-)similar to low-affinity P(i) transport in plant studies. The electrophysiological properties of HvPHT1;6, which locates to the plasma membrane when expressed in onion (Allium cepa) epidermal cells, are consistent with its suggested role in the remobilization of P(i) in barley plants.
Ai,
Two rice phosphate transporters, OsPht1;2 and OsPht1;6, have different functions and kinetic properties in uptake and translocation.
2009, Pubmed,
Xenbase
Ai,
Two rice phosphate transporters, OsPht1;2 and OsPht1;6, have different functions and kinetic properties in uptake and translocation.
2009,
Pubmed
,
Xenbase
Bacconi,
Renouncing electroneutrality is not free of charge: switching on electrogenicity in a Na+-coupled phosphate cotransporter.
2005,
Pubmed
,
Xenbase
Cameron,
The subcellular concentration of ions and elements in thin cryosections of onion root meristem cells. An electron-probe EDS study.
1984,
Pubmed
Curtis,
A gateway cloning vector set for high-throughput functional analysis of genes in planta.
2003,
Pubmed
Daram,
Functional analysis and cell-specific expression of a phosphate transporter from tomato.
1998,
Pubmed
Drew,
Uptake and long-distance transport of phosphate, potassium and chloride in relation to internal ion concentrations in barley: evidence of non-allosteric regulation.
1984,
Pubmed
Goldstein,
Phosphate Starvation Inducible Metabolism in Lycopersicon esculentum: I. Excretion of Acid Phosphatase by Tomato Plants and Suspension-Cultured Cells.
1988,
Pubmed
Guo,
Functional analysis of the Arabidopsis PHT4 family of intracellular phosphate transporters.
2008,
Pubmed
Hafke,
Thermodynamic battle for photosynthate acquisition between sieve tubes and adjoining parenchyma in transport phloem.
2005,
Pubmed
Huang,
Metabolite profiling reveals distinct changes in carbon and nitrogen metabolism in phosphate-deficient barley plants (Hordeum vulgare L.).
2008,
Pubmed
Lambers,
Root structure and functioning for efficient acquisition of phosphorus: Matching morphological and physiological traits.
2006,
Pubmed
Leggewie,
Two cDNAs from potato are able to complement a phosphate uptake-deficient yeast mutant: identification of phosphate transporters from higher plants.
1997,
Pubmed
Liu,
Closely related members of the Medicago truncatula PHT1 phosphate transporter gene family encode phosphate transporters with distinct biochemical activities.
2008,
Pubmed
Marschner,
Effect of mineral nutritional status on shoot-root partitioning of photoassimilates and cycling of mineral nutrients.
1996,
Pubmed
Marten,
AKT3, a phloem-localized K+ channel, is blocked by protons.
1999,
Pubmed
,
Xenbase
Miller,
Xenopus oocytes as an expression system for plant transporters.
2000,
Pubmed
,
Xenbase
Mitsukawa,
Overexpression of an Arabidopsis thaliana high-affinity phosphate transporter gene in tobacco cultured cells enhances cell growth under phosphate-limited conditions.
1997,
Pubmed
Muchhal,
Phosphate transporters from the higher plant Arabidopsis thaliana.
1996,
Pubmed
Mudge,
Expression analysis suggests novel roles for members of the Pht1 family of phosphate transporters in Arabidopsis.
2002,
Pubmed
Nelson,
A multicolored set of in vivo organelle markers for co-localization studies in Arabidopsis and other plants.
2007,
Pubmed
Piñeros,
Novel properties of the wheat aluminum tolerance organic acid transporter (TaALMT1) revealed by electrophysiological characterization in Xenopus Oocytes: functional and structural implications.
2008,
Pubmed
,
Xenbase
Rae,
Characterization of two phosphate transporters from barley; evidence for diverse function and kinetic properties among members of the Pht1 family.
2003,
Pubmed
Schachtman,
Phosphorus Uptake by Plants: From Soil to Cell.
1998,
Pubmed
Shenoy,
Enhancing plant phosphorus use efficiency for sustainable cropping.
2005,
Pubmed
Shin,
Loss of At4 function impacts phosphate distribution between the roots and the shoots during phosphate starvation.
2006,
Pubmed
Stegen,
Swelling-induced taurine release without chloride channel activity in Xenopus laevis oocytes expressing anion channels and transporters.
2000,
Pubmed
,
Xenbase
Vincill,
GmN70 and LjN70. Anion transporters of the symbiosome membrane of nodules with a transport preference for nitrate.
2005,
Pubmed
,
Xenbase
Virkki,
Substrate interactions in the human type IIa sodium-phosphate cotransporter (NaPi-IIa).
2005,
Pubmed
,
Xenbase
Weber,
Ion currents of Xenopus laevis oocytes: state of the art.
1999,
Pubmed
,
Xenbase
Weber,
Influence of extracellular Ca2+ on endogenous Cl- channels in Xenopus oocytes.
1995,
Pubmed
,
Xenbase