Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
Biophys J
2006 Mar 01;905:1797-803. doi: 10.1529/biophysj.105.073502.
Show Gene links
Show Anatomy links
Subcellular in vivo 1H MR spectroscopy of Xenopus laevis oocytes.
Lee SC
,
Cho JH
,
Mietchen D
,
Kim YS
,
Hong KS
,
Lee C
,
Kang D
,
Park KD
,
Choi BS
,
Cheong C
.
???displayArticle.abstract???
In vivo magnetic resonance (MR) spectra are typically obtained from voxels whose spatial dimensions far exceed those of the cells they contain. This study was designed to evaluate the potential of localized MR spectroscopy to investigate subcellular phenomena. Using a high magnetic field and a home-built microscopy probe with large gradient field strengths, we achieved voxel sizes of (180 microm)3. In the large oocytes of the frog Xenopus laevis, this was small enough to allow the recording of the first compartment-selective in vivo MR spectra from the animal and vegetal cytoplasm as well as the nucleus. The two cytoplasmic regions differed in their lipid contents and NMR lineshape characteristics-differences that are not detectable with whole-cell NMR techniques. In the nucleus, the signal appeared to be dominated by water, whereas other contributions were negligible. We also used localized spectroscopy to monitor the uptake of diminazene acturate, an antitrypanosomal agent, into compartments of a single living oocyte. The resulting spectra from the nucleus and cytoplasm revealed different uptake kinetics for the two components of the drug and demonstrate that MR technology is on the verge of becoming a tool for cell biology.
Aguayo,
Nuclear magnetic resonance imaging of a single cell.
, Pubmed,
Xenbase
Aguayo,
Nuclear magnetic resonance imaging of a single cell.
,
Pubmed
,
Xenbase
Allen,
Cellular delivery of MRI contrast agents.
2004,
Pubmed
Barker,
Single-voxel proton MRS of the human brain at 1.5T and 3.0T.
2001,
Pubmed
Bashirullah,
RNA localization in development.
1998,
Pubmed
,
Xenbase
Benveniste,
MR microscopy and high resolution small animal MRI: applications in neuroscience research.
2002,
Pubmed
Bottomley,
Spatial localization in NMR spectroscopy in vivo.
1987,
Pubmed
Bowtell,
NMR microscopy of single neurons using spin echo and line narrowed 2DFT imaging.
1995,
Pubmed
Danilchik,
Differentiation of the animal-vegetal axis in Xenopus laevis oocytes. I. Polarized intracellular translocation of platelets establishes the yolk gradient.
1987,
Pubmed
,
Xenbase
Dascal,
The use of Xenopus oocytes for the study of ion channels.
1987,
Pubmed
,
Xenbase
Grant,
NMR spectroscopy of single neurons.
2000,
Pubmed
Gruetter,
Broadband decoupled, 1H-localized 13C MRS of the human brain at 4 Tesla.
1996,
Pubmed
Hakumäki,
1H NMR visible lipids in the life and death of cells.
2000,
Pubmed
Horowitz,
The permeability of the amphibian oocyte nucleus, in situ.
1972,
Pubmed
Horowitz,
Intracellular monosaccharide and amino acid concentrations and activities and the mechanisms of insulin action.
1981,
Pubmed
Hoult,
The signal-to-noise ratio of the nuclear magnetic resonance experiment. 1976.
2011,
Pubmed
Hsu,
A study of diffusion isotropy in single neurons by using NMR microscopy.
1997,
Pubmed
Kusano,
Acetylcholine receptors in the oocyte membrane.
,
Pubmed
,
Xenbase
Lee,
One micrometer resolution NMR microscopy.
2001,
Pubmed
Lee,
Observations on the subcellular distribution of the ammonium ion in maize root tissue using in-vivo (14)N-nuclear magnetic resonance spectroscopy.
1991,
Pubmed
Lee,
Localized measurement of kinase activation in oocytes of Xenopus laevis.
1999,
Pubmed
,
Xenbase
Mamman,
Comparative pharmacokinetics of diminazene in noninfected Boran (Bos indicus) cattle and Boran cattle infected with Trypanosoma congolense.
1993,
Pubmed
McCoy,
The effect of blood flow modification on intra- and extracellular pH measured by 31P magnetic resonance spectroscopy in murine tumours.
1995,
Pubmed
Meylan,
Atom/fragment contribution method for estimating octanol-water partition coefficients.
1995,
Pubmed
Minard,
Picoliter (1)H NMR spectroscopy.
2002,
Pubmed
,
Xenbase
Orive,
Drug delivery in biotechnology: present and future.
2003,
Pubmed
Pfeuffer,
Extracellular-intracellular distribution of glucose and lactate in the rat brain assessed noninvasively by diffusion-weighted 1H nuclear magnetic resonance spectroscopy in vivo.
2000,
Pubmed
Pilch,
Berenil [1,3-bis(4'-amidinophenyl)triazene] binding to DNA duplexes and to a RNA duplex: evidence for both intercalative and minor groove binding properties.
1995,
Pubmed
Päuser,
Study of the membrane permeability of a paramagnetic metal complex on single cells by NMR microscopy.
1993,
Pubmed
,
Xenbase
Päuser,
Estimation of water content and water mobility in the nucleus and cytoplasm of Xenopus laevis oocytes by NMR microscopy.
1995,
Pubmed
,
Xenbase
Schoeniger,
Relaxation-time and diffusion NMR microscopy of single neurons.
1994,
Pubmed
Sehy,
Water and lipid MRI of the Xenopus oocyte.
2001,
Pubmed
,
Xenbase
Sehy,
Apparent diffusion of water, ions, and small molecules in the Xenopus oocyte is consistent with Brownian displacement.
2002,
Pubmed
,
Xenbase
Shen,
Developmentally-regulated packaging of mitochondrial DNA by the HMG-box protein mtTFA during Xenopus oogenesis.
2001,
Pubmed
,
Xenbase
Szczepaniak,
Measurement of intracellular triglyceride stores by H spectroscopy: validation in vivo.
1999,
Pubmed
Territo,
Whole-body composition of Xenopus laevis larvae: implications for lean body mass during development.
1998,
Pubmed
,
Xenbase
Tkác,
Highly resolved in vivo 1H NMR spectroscopy of the mouse brain at 9.4 T.
2004,
Pubmed
Tourte,
Heterogeneous distribution and replication activity of mitochondria in Xenopus laevis oocytes.
1984,
Pubmed
,
Xenbase
Van Zijl,
Complete separation of intracellular and extracellular information in NMR spectra of perfused cells by diffusion-weighted spectroscopy.
1991,
Pubmed
Volodina,
Apparent mitochondrial asymmetry in Xenopus eggs.
2003,
Pubmed
,
Xenbase
Wellard,
Factors affecting 133Cs chemical shifts in erythrocytes from cesium-fed rats.
1994,
Pubmed
Weybright,
Gradient, high-resolution, magic angle spinning 1H nuclear magnetic resonance spectroscopy of intact cells.
1998,
Pubmed
