Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
Expression of human cathepsin D in Xenopus oocytes: phosphorylation and intracellular targeting.
Faust PL
,
Wall DA
,
Perara E
,
Lingappa VR
,
Kornfeld S
.
Abstract
We have obtained expression of a cDNA clone for human cathepsin D in Xenopus laevis oocytes. Biosynthetic studies with [35S]methionine labeling demonstrated that most of the cathepsin D remained intracellular and underwent proteolytic cleavage, converting a precursor of Mr 47,000 D to a mature form of Mr 39,000 D with processing intermediates of Mr 43,000-41,000 D. greater than 90% of the cathepsin D synthesized by oocytes bound to a mannose 6-phosphate (Man-6-P) receptor affinity column, indicating the presence of phosphomannosyl residues. An analysis of [2-3H]mannose-labeled oligosaccharides directly demonstrated phosphomannosyl residues on cathepsin D. Sucrose-gradient fractionation, performed to define the membranous compartments that cathepsin D traversed during its biosynthesis, demonstrated that cathepsin D is targeted to a subpopulation of yolk platelets, the oocyte equivalent of a lysosome. Xenopus oocytes were able to endocytose lysosomal enzymes from the medium and this uptake was inhibited by Man-6-P, thus demonstrating the presence of Man-6-P receptors in these cells. Therefore, the entire Man-6-P dependent pathway for targeting of lysosomal enzymes is present in the oocytes. Xenopus oocytes should be a useful system for examining signals responsible for the specific targeting of lysosomal enzymes to lysosomes.
Erickson,
Carboxyl-terminal proteolytic processing during biosynthesis of the lysosomal enzymes beta-glucuronidase and cathepsin D.
1983, Pubmed
Erickson,
Carboxyl-terminal proteolytic processing during biosynthesis of the lysosomal enzymes beta-glucuronidase and cathepsin D.
1983,
Pubmed
Faust,
Renin, a secretory glycoprotein, acquires phosphomannosyl residues.
1987,
Pubmed
,
Xenbase
Faust,
Cloning and sequence analysis of cDNA for human cathepsin D.
1985,
Pubmed
Gabel,
Lysosomal enzyme oligosaccharide phosphorylation in mouse lymphoma cells: specificity and kinetics of binding to the mannose 6-phosphate receptor in vivo.
1982,
Pubmed
Gabel,
Identification of methylphosphomannosyl residues as components of the high mannose oligosaccharides of Dictyostelium discoideum glycoproteins.
1984,
Pubmed
Gieselmann,
Processing of human cathepsin D in lysosomes in vitro.
1985,
Pubmed
Hall,
Enzymic diagnosis of the genetic mucopolysaccharide storage disorders.
1978,
Pubmed
Hasilik,
Biosynthesis of lysosomal enzymes in fibroblasts. Phosphorylation of mannose residues.
1980,
Pubmed
Hasilik,
Lysosomal enzyme precursors in human fibroblasts. Activation of cathepsin D precursor in vitro and activity of beta-hexosaminidase A precursor towards ganglioside GM2.
1982,
Pubmed
Hasilik,
Biosynthesis of lysosomal enzymes in fibroblasts. Synthesis as precursors of higher molecular weight.
1980,
Pubmed
Laemmli,
Cleavage of structural proteins during the assembly of the head of bacteriophage T4.
1970,
Pubmed
Lang,
Glycoprotein phosphorylation in simple eucaryotic organisms. Identification of UDP-GlcNAc:glycoprotein N-acetylglucosamine-1-phosphotransferase activity and analysis of substrate specificity.
1986,
Pubmed
Lang,
Lysosomal enzyme phosphorylation. Recognition of a protein-dependent determinant allows specific phosphorylation of oligosaccharides present on lysosomal enzymes.
1984,
Pubmed
Melton,
Efficient in vitro synthesis of biologically active RNA and RNA hybridization probes from plasmids containing a bacteriophage SP6 promoter.
1984,
Pubmed
Reitman,
UDP-N-acetylglucosamine:glycoprotein N-acetylglucosamine-1-phosphotransferase. Proposed enzyme for the phosphorylation of the high mannose oligosaccharide units of lysosomal enzymes.
1981,
Pubmed
Reitman,
Lysosomal enzyme targeting. N-Acetylglucosaminylphosphotransferase selectively phosphorylates native lysosomal enzymes.
1981,
Pubmed
Rome,
Two species of lysosomal organelles in cultured human fibroblasts.
1979,
Pubmed
Rosenfeld,
Biosynthesis of lysosomal hydrolases: their synthesis in bound polysomes and the role of co- and post-translational processing in determining their subcellular distribution.
1982,
Pubmed
Sahagian,
Characterization of a membrane-associated receptor from bovine liver that binds phosphomannosyl residues of bovine testicular beta-galactosidase.
1981,
Pubmed
Sanger,
DNA sequencing with chain-terminating inhibitors.
1977,
Pubmed
Takahashi,
Cathepsin D from porcine and bovine spleen.
1981,
Pubmed
Trowbridge,
Expression of Thy-1 glycoprotein on lectin-resistant lymphoma cell lines.
1978,
Pubmed
Varki,
The spectrum of anionic oligosaccharides released by endo-beta-N-acetylglucosaminidase H from glycoproteins. Structural studies and interactions with the phosphomannosyl receptor.
1983,
Pubmed
Varki,
Structural studies of phosphorylated high mannose-type oligosaccharides.
1980,
Pubmed
Wall,
Multivesicular bodies play a key role in vitellogenin endocytosis by Xenopus oocytes.
1987,
Pubmed
,
Xenbase
Wall,
An unusual lysosome compartment involved in vitellogenin endocytosis by Xenopus oocytes.
1985,
Pubmed
,
Xenbase
Wallace,
Protein incorporation by isolated amphibian oocytes. V. Specificity for vitellogenin incorporation.
1976,
Pubmed
,
Xenbase