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Development 2000 Mar 01;1276:1173-83. doi: 10.1242/dev.127.6.1173.
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Endodermal Nodal-related signals and mesoderm induction in Xenopus.

Agius E , Oelgeschläger M , Wessely O , Kemp C , De Robertis EM .

In Xenopus, mesoderm induction by endoderm at the blastula stage is well documented, but the molecular nature of the endogenous inductive signals remains unknown. The carboxy-terminal fragment of Cerberus, designated Cer-S, provides a specific secreted antagonist of mesoderm-inducing Xenopus Nodal-Related (Xnr) factors. Cer-S does not inhibit signalling by other mesoderm inducers such as Activin, Derrière, Vg1 and BMP4, nor by the neural inducer Xnr3. In the present study we show that Cer-S blocks the induction of both dorsal and ventral mesoderm in animal-vegetal Nieuwkoop-type recombinants. During blastula stages Xnr1, Xnr2 and Xnr4 are expressed in a dorsal to ventral gradient in endodermal cells. Dose-response experiments using cer-S mRNA injections support the existence of an endogenous activity gradient of Xnrs. Xnr expression at blastula can be activated by the vegetal determinants VegT and Vg1 acting in synergy with dorsal (beta)-catenin. The data support a modified model for mesoderm induction in Xenopus, in which mesoderm induction is mediated by a gradient of multiple Nodal-related signals released by endoderm at the blastula stage.

PubMed ID: 10683171
PMC ID: PMC2292107
Article link: Development
Grant support: [+]

Species referenced: Xenopus
Genes referenced: bmp4 cer1 chrd fst gdf1 gsc nodal nodal1 nodal2 nodal3.1 nodal3.2 odc1 sia1 tbx2 tbxt vegt

Article Images: [+] show captions
References [+] :
Belo, Cerberus-like is a secreted factor with neutralizing activity expressed in the anterior primitive endoderm of the mouse gastrula. 1997, Pubmed, Xenbase