Click here to close Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly. We suggest using a current version of Chrome, FireFox, or Safari.
XB-ART-37961
Neuron 2008 May 08;583:362-73. doi: 10.1016/j.neuron.2008.04.012.
Show Gene links Show Anatomy links

A yeast genetic screen reveals a critical role for the pore helix domain in TRP channel gating.

Myers BR , Bohlen CJ , Julius D .


Abstract
TRP cation channels function as cellular sensors in uni- and multicellular eukaryotes. Despite intensive study, the mechanisms of TRP channel activation by chemical or physical stimuli remain poorly understood. To identify amino acid residues crucial for TRP channel gating, we developed an unbiased, high-throughput genetic screen in yeast that uncovered rare, constitutively active mutants of the capsaicin receptor, TRPV1. We show that mutations within the pore helix domain dramatically increase basal channel activity and responsiveness to chemical and thermal stimuli. Mutation of corresponding residues within two related TRPV channels leads to comparable effects on their activation properties. Our data suggest that conformational changes in the outer pore region are critical for determining the balance between open and closed states, providing evidence for a general role for this domain in TRP channel activation.

PubMed ID: 18466747
PMC ID: PMC2422846
Article link: Neuron
Grant support: [+]

Species referenced: Xenopus laevis
Genes referenced: trpv1

References [+] :
Ahern, A cation-pi interaction between extracellular TEA and an aromatic residue in potassium channels. 2006, Pubmed