Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
Mol Cell Biol
1993 Jun 01;136:3744-55. doi: 10.1128/mcb.13.6.3744-3755.1993.
Show Gene links
Show Anatomy links
Full activation of p34CDC28 histone H1 kinase activity is unable to promote entry into mitosis in checkpoint-arrested cells of the yeast Saccharomyces cerevisiae.
Stueland CS
,
Lew DJ
,
Cismowski MJ
,
Reed SI
.
???displayArticle.abstract???
In most cells, mitosis is dependent upon completion of DNA replication. The feedback mechanisms that prevent entry into mitosis by cells with damaged or incompletely replicated DNA have been termed checkpoint controls. Studies with the fission yeast Schizosaccharomyces pombe and Xenopus egg extracts have shown that checkpoint controls prevent activation of the master regulatory protein kinase, p34cdc2, that normally triggers entry into mitosis. This is achieved through inhibitory phosphorylation of the Tyr-15 residue of p34cdc2. However, studies with the budding yeast Saccharomyces cerevisiae have shown that phosphorylation of this residue is not essential for checkpoint controls to prevent mitosis. We have investigated the basis for checkpoint controls in this organism and show that these controls can prevent entry into mitosis even in cells which have fully activated the cyclin B (Clb)-associated forms of the budding yeast homolog of p34cdc2, p34CDC28, as assayed by histone H1 kinase activity. However, the active complexes in checkpoint-arrested cells are smaller than those in cycling cells, suggesting that assembly of mitosis-inducing complexes requires additional steps following histone H1 kinase activation.
Amon,
Regulation of p34CDC28 tyrosine phosphorylation is not required for entry into mitosis in S. cerevisiae.
1992, Pubmed,
Xenbase
Amon,
Regulation of p34CDC28 tyrosine phosphorylation is not required for entry into mitosis in S. cerevisiae.
1992,
Pubmed
,
Xenbase
Dasso,
Completion of DNA replication is monitored by a feedback system that controls the initiation of mitosis in vitro: studies in Xenopus.
1990,
Pubmed
,
Xenbase
Dorée,
Control of M-phase by maturation-promoting factor.
1990,
Pubmed
Enoch,
Mutation of fission yeast cell cycle control genes abolishes dependence of mitosis on DNA replication.
1990,
Pubmed
Fitch,
Characterization of four B-type cyclin genes of the budding yeast Saccharomyces cerevisiae.
1992,
Pubmed
Ghiara,
A cyclin B homolog in S. cerevisiae: chronic activation of the Cdc28 protein kinase by cyclin prevents exit from mitosis.
1991,
Pubmed
Gould,
Tyrosine phosphorylation of the fission yeast cdc2+ protein kinase regulates entry into mitosis.
1989,
Pubmed
Grandin,
Differential function and expression of Saccharomyces cerevisiae B-type cyclins in mitosis and meiosis.
1993,
Pubmed
Hadwiger,
The Saccharomyces cerevisiae CKS1 gene, a homolog of the Schizosaccharomyces pombe suc1+ gene, encodes a subunit of the Cdc28 protein kinase complex.
1989,
Pubmed
Hartwell,
Checkpoints: controls that ensure the order of cell cycle events.
1989,
Pubmed
Hunt,
Maturation promoting factor, cyclin and the control of M-phase.
1989,
Pubmed
Kumagai,
The cdc25 protein controls tyrosine dephosphorylation of the cdc2 protein in a cell-free system.
1991,
Pubmed
,
Xenbase
Labbé,
MPF from starfish oocytes at first meiotic metaphase is a heterodimer containing one molecule of cdc2 and one molecule of cyclin B.
1989,
Pubmed
Lew,
Different G1 cyclins control the timing of cell cycle commitment in mother and daughter cells of the budding yeast S. cerevisiae.
1992,
Pubmed
Lew,
Morphogenesis in the yeast cell cycle: regulation by Cdc28 and cyclins.
1993,
Pubmed
Lundgren,
mik1 and wee1 cooperate in the inhibitory tyrosine phosphorylation of cdc2.
1991,
Pubmed
Maller,
Mitotic control.
1991,
Pubmed
Murray,
Dominoes and clocks: the union of two views of the cell cycle.
1989,
Pubmed
Nigg,
The substrates of the cdc2 kinase.
1991,
Pubmed
Nurse,
Universal control mechanism regulating onset of M-phase.
1990,
Pubmed
Pringle,
Immunofluorescence methods for yeast.
1991,
Pubmed
Richardson,
Cyclin-B homologs in Saccharomyces cerevisiae function in S phase and in G2.
1992,
Pubmed
Richardson,
An essential G1 function for cyclin-like proteins in yeast.
1989,
Pubmed
Russell,
Conservation of mitotic controls in fission and budding yeasts.
1989,
Pubmed
Sclafani,
Yeast gene CDC8 encodes thymidylate kinase and is complemented by herpes thymidine kinase gene TK.
1984,
Pubmed
Smythe,
Coupling of mitosis to the completion of S phase in Xenopus occurs via modulation of the tyrosine kinase that phosphorylates p34cdc2.
1992,
Pubmed
,
Xenbase
Sorger,
S-phase feedback control in budding yeast independent of tyrosine phosphorylation of p34cdc28.
1992,
Pubmed
,
Xenbase
Surana,
The role of CDC28 and cyclins during mitosis in the budding yeast S. cerevisiae.
1991,
Pubmed
Weinert,
The RAD9 gene controls the cell cycle response to DNA damage in Saccharomyces cerevisiae.
1988,
Pubmed
Wittenberg,
Subcellular localization of a protein kinase required for cell cycle initiation in Saccharomyces cerevisiae: evidence for an association between the CDC28 gene product and the insoluble cytoplasmic matrix.
1987,
Pubmed
Wittenberg,
Control of the yeast cell cycle is associated with assembly/disassembly of the Cdc28 protein kinase complex.
1988,
Pubmed
