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J Biol Chem
2011 Jan 14;2862:1181-8. doi: 10.1074/jbc.M110.185496.
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Light modulation of cellular cAMP by a small bacterial photoactivated adenylyl cyclase, bPAC, of the soil bacterium Beggiatoa.
Stierl M
,
Stumpf P
,
Udwari D
,
Gueta R
,
Hagedorn R
,
Losi A
,
Gärtner W
,
Petereit L
,
Efetova M
,
Schwarzel M
,
Oertner TG
,
Nagel G
,
Hegemann P
.
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The recent success of channelrhodopsin in optogenetics has also caused increasing interest in enzymes that are directly activated by light. We have identified in the genome of the bacterium Beggiatoa a DNA sequence encoding an adenylyl cyclase directly linked to a BLUF (blue light receptor using FAD) type light sensor domain. In Escherichia coli and Xenopus oocytes, this photoactivated adenylyl cyclase (bPAC) showed cyclase activity that is low in darkness but increased 300-fold in the light. This enzymatic activity decays thermally within 20 s in parallel with the red-shifted BLUF photointermediate. bPAC is well expressed in pyramidal neurons and, in combination with cyclic nucleotide gated channels, causes efficient light-induced depolarization. In the Drosophila central nervous system, bPAC mediates light-dependent cAMP increase and behavioral changes in freely moving animals. bPAC seems a perfect optogenetic tool for light modulation of cAMP in neuronal cells and tissues and for studying cAMP-dependent processes in live animals.
Bailey,
Toward a molecular definition of long-term memory storage.
1996, Pubmed
Bailey,
Toward a molecular definition of long-term memory storage.
1996,
Pubmed
Bonetti,
The role of key amino acids in the photoactivation pathway of the Synechocystis Slr1694 BLUF domain.
2009,
Pubmed
Chen,
The mechanism of cAMP-mediated enhancement at a cerebellar synapse.
1997,
Pubmed
Christie,
Phototropin LOV domains exhibit distinct roles in regulating photoreceptor function.
2002,
Pubmed
Dunn,
Imaging second messenger dynamics in developing neural circuits.
2008,
Pubmed
Gomelsky,
BLUF: a novel FAD-binding domain involved in sensory transduction in microorganisms.
2002,
Pubmed
Gorostiza,
Optical switches for remote and noninvasive control of cell signaling.
2008,
Pubmed
Hinck,
Physiological adaptation of a nitrate-storing Beggiatoa sp. to diel cycling in a phototrophic hypersaline mat.
2007,
Pubmed
Iseki,
A blue-light-activated adenylyl cyclase mediates photoavoidance in Euglena gracilis.
2002,
Pubmed
Karimova,
A bacterial two-hybrid system based on a reconstituted signal transduction pathway.
1998,
Pubmed
Liman,
Subunit stoichiometry of a mammalian K+ channel determined by construction of multimeric cDNAs.
1992,
Pubmed
,
Xenbase
Linder,
The class III adenylyl cyclases: multi-purpose signalling modules.
2003,
Pubmed
Looser,
Mechanistic insights in light-induced cAMP production by photoactivated adenylyl cyclase alpha (PACalpha).
2009,
Pubmed
,
Xenbase
Makhinson,
Adenylyl cyclase activation modulates activity-dependent changes in synaptic strength and Ca2+/calmodulin-dependent kinase II autophosphorylation.
1999,
Pubmed
Masuda,
AppA is a blue light photoreceptor that antirepresses photosynthesis gene expression in Rhodobacter sphaeroides.
2002,
Pubmed
Matsuoka,
Blue light-regulated molecular switch of Ser/Thr kinase in phototropin.
2005,
Pubmed
Miller,
Optogenetics. Shining new light on neural circuits.
2006,
Pubmed
Mills,
Identification of members of the metabolically active microbial populations associated with Beggiatoa species mat communities from Gulf of Mexico cold-seep sediments.
2004,
Pubmed
Möglich,
Design and signaling mechanism of light-regulated histidine kinases.
2009,
Pubmed
Møller,
Oxygen Responses and Mat Formation by Beggiatoa spp.
1985,
Pubmed
Ntefidou,
Photoactivated adenylyl cyclase controls phototaxis in the flagellate Euglena gracilis.
2003,
Pubmed
Otmakhov,
Forskolin-induced LTP in the CA1 hippocampal region is NMDA receptor dependent.
2004,
Pubmed
Rich,
In vivo assessment of local phosphodiesterase activity using tailored cyclic nucleotide-gated channels as cAMP sensors.
2001,
Pubmed
Schoenenberger,
Channelrhodopsin as a tool to investigate synaptic transmission and plasticity.
2011,
Pubmed
Schoenenberger,
Temporal control of immediate early gene induction by light.
2009,
Pubmed
Schröder-Lang,
Fast manipulation of cellular cAMP level by light in vivo.
2007,
Pubmed
,
Xenbase
Schroll,
Light-induced activation of distinct modulatory neurons triggers appetitive or aversive learning in Drosophila larvae.
2006,
Pubmed
Stoppini,
A simple method for organotypic cultures of nervous tissue.
1991,
Pubmed
Strickland,
Light-activated DNA binding in a designed allosteric protein.
2008,
Pubmed
Weinreich,
Direct action of genistein on CFTR.
1997,
Pubmed
,
Xenbase
Wu,
A genetically encoded photoactivatable Rac controls the motility of living cells.
2009,
Pubmed
Zaccolo,
Discrete microdomains with high concentration of cAMP in stimulated rat neonatal cardiac myocytes.
2002,
Pubmed
Zhang,
Red-shifted optogenetic excitation: a tool for fast neural control derived from Volvox carteri.
2008,
Pubmed
,
Xenbase
