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XB-ART-28846
Gene 1986 Jan 01;491:1-8.
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High-level, heat-regulated synthesis of proteins in eukaryotic cells.

Dreano M , Brochot J , Myers A , Cheng-Meyer C , Rungger D , Voellmy R , Bromley P .


Abstract
Plasmids have been constructed in which promoters of 70-kDa heat-shock protein genes (hsp70) of human and Drosophila origin were linked to three different eukaryotic genes encoding human growth hormone (hGH), chicken lysozyme (cL) and a human influenza haemagglutinin (HA). Following transfection into widely divergent eukaryotic cells, the hybrid genes direct the transient, heat-regulated synthesis of the three proteins. hGH and cL are secreted into the medium. A human hsp70-hGH construct was used to establish stable mouse fibroblast lines that are capable of producing and secreting hGH at high levels following heat induction: hGH is secreted at a 500-1200-fold higher rate by heat-treated than by untreated cells.

PubMed ID: 3569912
Article link: Gene


Species referenced: Xenopus
Genes referenced: hsp70 hspa1l